Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney
Objective: To investigate the hepatorenoprotective effects of Origanum vulgare L. against finasteride-induced oxidative injury in the liver and kidney of mice. Methods: Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI/MS) analysis was utilized to yield a fingerprint of...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2021
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Online Access: | https://doi.org/10.4103/2221-1691.311755 https://doaj.org/article/bf3aff55426e4d01a59b75a64733e68a |
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ftdoajarticles:oai:doaj.org/article:bf3aff55426e4d01a59b75a64733e68a 2023-05-15T15:13:09+02:00 Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney Qi-Feng Sun Shi-Xiang Chen Zhang-Feng Tang Xiao-Yi Song Fa Jing Hao-Tian Wu Zhong-Yang Ding Attalla El-kott Diaa Massoud Heba Khalifa 2021-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.311755 https://doaj.org/article/bf3aff55426e4d01a59b75a64733e68a EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=5;spage=194;epage=204;aulast=Sun https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.311755 https://doaj.org/article/bf3aff55426e4d01a59b75a64733e68a Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 5, Pp 194-204 (2021) kidney liver origanum vulgare l. inflammation antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.4103/2221-1691.311755 2022-12-31T00:28:41Z Objective: To investigate the hepatorenoprotective effects of Origanum vulgare L. against finasteride-induced oxidative injury in the liver and kidney of mice. Methods: Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI/MS) analysis was utilized to yield a fingerprint of Origanum vulgare polyphenolic constituents. Thirty BALB/c mice received 0.5 mL/day distilled water, finasteride (25 mg/kg/day for 10 d), and 100, 200, or 400 mg/kg/day finasteride + Origanum vulgare extract with 6 mice per group for five weeks. On day 36, liver and kidney function as well as pro- and antiinflammatory (IFN-γ, IL-12, IL-6, TNF-α, IL-1β, and IL-10) cytokines were measured. The total antioxidant status, nitric oxide (NO), and malondialdehyde levels as well as the activities of NO synthase and catalase were also evaluated. Histopathological study was conducted to assess the effect of Origanum vulgare extract on finasteride-induced renal and hepatic toxicities. Results: Twenty-five major polyphenolic compounds were identified in the Origanum vulgare extract by LC-ESI/MS. Origanum vulgare extract, especially at 200 and 400 mg/kg/day doses, significantly improved liver and kidney biochemical indices, decreased inflammatory cytokines, increased total antioxidant status and NO synthase and catalase activities, as well as decreased plasma NO and malondialdehyde levels in a dose-dependent manner as compared to the finasteride group. Histopathological results further confirmed the protective effect of Origanum vulgare extract. Conclusions: Origanum vulgare extract ameliorates finasteride-induced hepatic and renal biochemical and histopathological alterations, and restores antioxidant/oxidant balance. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 11 5 194 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
kidney liver origanum vulgare l. inflammation antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
kidney liver origanum vulgare l. inflammation antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Qi-Feng Sun Shi-Xiang Chen Zhang-Feng Tang Xiao-Yi Song Fa Jing Hao-Tian Wu Zhong-Yang Ding Attalla El-kott Diaa Massoud Heba Khalifa Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney |
topic_facet |
kidney liver origanum vulgare l. inflammation antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the hepatorenoprotective effects of Origanum vulgare L. against finasteride-induced oxidative injury in the liver and kidney of mice. Methods: Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI/MS) analysis was utilized to yield a fingerprint of Origanum vulgare polyphenolic constituents. Thirty BALB/c mice received 0.5 mL/day distilled water, finasteride (25 mg/kg/day for 10 d), and 100, 200, or 400 mg/kg/day finasteride + Origanum vulgare extract with 6 mice per group for five weeks. On day 36, liver and kidney function as well as pro- and antiinflammatory (IFN-γ, IL-12, IL-6, TNF-α, IL-1β, and IL-10) cytokines were measured. The total antioxidant status, nitric oxide (NO), and malondialdehyde levels as well as the activities of NO synthase and catalase were also evaluated. Histopathological study was conducted to assess the effect of Origanum vulgare extract on finasteride-induced renal and hepatic toxicities. Results: Twenty-five major polyphenolic compounds were identified in the Origanum vulgare extract by LC-ESI/MS. Origanum vulgare extract, especially at 200 and 400 mg/kg/day doses, significantly improved liver and kidney biochemical indices, decreased inflammatory cytokines, increased total antioxidant status and NO synthase and catalase activities, as well as decreased plasma NO and malondialdehyde levels in a dose-dependent manner as compared to the finasteride group. Histopathological results further confirmed the protective effect of Origanum vulgare extract. Conclusions: Origanum vulgare extract ameliorates finasteride-induced hepatic and renal biochemical and histopathological alterations, and restores antioxidant/oxidant balance. |
format |
Article in Journal/Newspaper |
author |
Qi-Feng Sun Shi-Xiang Chen Zhang-Feng Tang Xiao-Yi Song Fa Jing Hao-Tian Wu Zhong-Yang Ding Attalla El-kott Diaa Massoud Heba Khalifa |
author_facet |
Qi-Feng Sun Shi-Xiang Chen Zhang-Feng Tang Xiao-Yi Song Fa Jing Hao-Tian Wu Zhong-Yang Ding Attalla El-kott Diaa Massoud Heba Khalifa |
author_sort |
Qi-Feng Sun |
title |
Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney |
title_short |
Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney |
title_full |
Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney |
title_fullStr |
Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney |
title_full_unstemmed |
Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney |
title_sort |
origanum vulgare l. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/2221-1691.311755 https://doaj.org/article/bf3aff55426e4d01a59b75a64733e68a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 5, Pp 194-204 (2021) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=5;spage=194;epage=204;aulast=Sun https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.311755 https://doaj.org/article/bf3aff55426e4d01a59b75a64733e68a |
op_doi |
https://doi.org/10.4103/2221-1691.311755 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
11 |
container_issue |
5 |
container_start_page |
194 |
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1766343742323687424 |