Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves
Objective: To determine the anti-cancer effect of aronia leaf extract on SK-Hep1 cells using migration, metallo metrix proteinase-2/-9 (MMP-2/-9) and MT-1 MMP expression and to evaluate the anti-inflammatory activities of the leaf extract. Methods: The effect of aronia leaf extract on cancer prevent...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:bf1bea50d867472f834585cc05b2c8a8 2023-05-15T15:17:46+02:00 Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves Nhuan Do Thi Eun-Sun Hwang 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.248095 https://doaj.org/article/bf1bea50d867472f834585cc05b2c8a8 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=12;spage=586;epage=592;aulast=Thi https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.248095 https://doaj.org/article/bf1bea50d867472f834585cc05b2c8a8 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 12, Pp 586-592 (2018) aronia leaf cancer metatasis lipopolysaccharides-induced inflammation Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.248095 2022-12-30T23:55:10Z Objective: To determine the anti-cancer effect of aronia leaf extract on SK-Hep1 cells using migration, metallo metrix proteinase-2/-9 (MMP-2/-9) and MT-1 MMP expression and to evaluate the anti-inflammatory activities of the leaf extract. Methods: The effect of aronia leaf extract on cancer prevention was investigated. SK-Hep1 human liver cancer cell line was treated with aronia leaf extract at various concentractions. MTT assay was used to measure cancer cell growth inhibition, and wound migration assay was used for metastasis determination. The expression of MMP-2/-9 was measured at the protein level using zymography and the expression of MMP-2/-9 and MT-1 MMP was examined at the gene level by RT-PCR. Raw 264.7 macrophage cells were stimulated with lipopolysaccharides to induce inflammation, and then the inhibition of inflammation was evaluated by treatment of aronia leaf extract. Expressions of interleukin-6, tumor factor-α, and nitric oxide (NO) were also determined. Results: SK-Hep1 cell growth was inhibited in proportion to the concentration of aronia leaf extract. In migration assay, aronia leaf extract showed 61.3%-96.3% wound size inhibtion after treating 50-200 μg/mL of aronia leaf extract for 24 h. At the protein level, the expression of MMP-2 and MMP-9 decreased as the concentration of aronia leaf extract treated with SK-Hep1 cells increased. In addition, the same pattern as in the protein was also observed in the mRNA levels. The expressions of MMP-2 and MMP-9 protein were inhibited by 92.2% and 53.8%, respectively after treatment with 200 μg/mL aronia leaf extract. In addition, Raw 264.7 cells treated with aronia leaf extract did not affect cell survival. There was dose dependent inhibition of interleukine-6, tumor necrosis factor-α and nitric oxide after treating aronia leaf extract in lipopolysaccharides-treated Raw 264.7 cell. Conclusions: The results show that aronia leaf has anticancer and and antimetastatic properties in SK-Hep1 and Raw 264.7 cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 12 586 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
aronia leaf cancer metatasis lipopolysaccharides-induced inflammation Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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aronia leaf cancer metatasis lipopolysaccharides-induced inflammation Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nhuan Do Thi Eun-Sun Hwang Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves |
topic_facet |
aronia leaf cancer metatasis lipopolysaccharides-induced inflammation Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the anti-cancer effect of aronia leaf extract on SK-Hep1 cells using migration, metallo metrix proteinase-2/-9 (MMP-2/-9) and MT-1 MMP expression and to evaluate the anti-inflammatory activities of the leaf extract. Methods: The effect of aronia leaf extract on cancer prevention was investigated. SK-Hep1 human liver cancer cell line was treated with aronia leaf extract at various concentractions. MTT assay was used to measure cancer cell growth inhibition, and wound migration assay was used for metastasis determination. The expression of MMP-2/-9 was measured at the protein level using zymography and the expression of MMP-2/-9 and MT-1 MMP was examined at the gene level by RT-PCR. Raw 264.7 macrophage cells were stimulated with lipopolysaccharides to induce inflammation, and then the inhibition of inflammation was evaluated by treatment of aronia leaf extract. Expressions of interleukin-6, tumor factor-α, and nitric oxide (NO) were also determined. Results: SK-Hep1 cell growth was inhibited in proportion to the concentration of aronia leaf extract. In migration assay, aronia leaf extract showed 61.3%-96.3% wound size inhibtion after treating 50-200 μg/mL of aronia leaf extract for 24 h. At the protein level, the expression of MMP-2 and MMP-9 decreased as the concentration of aronia leaf extract treated with SK-Hep1 cells increased. In addition, the same pattern as in the protein was also observed in the mRNA levels. The expressions of MMP-2 and MMP-9 protein were inhibited by 92.2% and 53.8%, respectively after treatment with 200 μg/mL aronia leaf extract. In addition, Raw 264.7 cells treated with aronia leaf extract did not affect cell survival. There was dose dependent inhibition of interleukine-6, tumor necrosis factor-α and nitric oxide after treating aronia leaf extract in lipopolysaccharides-treated Raw 264.7 cell. Conclusions: The results show that aronia leaf has anticancer and and antimetastatic properties in SK-Hep1 and Raw 264.7 cells. |
format |
Article in Journal/Newspaper |
author |
Nhuan Do Thi Eun-Sun Hwang |
author_facet |
Nhuan Do Thi Eun-Sun Hwang |
author_sort |
Nhuan Do Thi |
title |
Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves |
title_short |
Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves |
title_full |
Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves |
title_fullStr |
Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves |
title_full_unstemmed |
Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves |
title_sort |
anti-cancer and anti-inflammatory activities of aronia (aronia melanocarpa) leaves |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.248095 https://doaj.org/article/bf1bea50d867472f834585cc05b2c8a8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 12, Pp 586-592 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=12;spage=586;epage=592;aulast=Thi https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.248095 https://doaj.org/article/bf1bea50d867472f834585cc05b2c8a8 |
op_doi |
https://doi.org/10.4103/2221-1691.248095 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
12 |
container_start_page |
586 |
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1766348004100407296 |