Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms
Abstract Background SINEs (Short INterspersed Elements) are homoplasy-free and co-dominant genetic markers which are considered to represent useful tools for population genetic studies, and could help clarifying the speciation processes ongoing within the major malaria vector in Africa, Anopheles ga...
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ftdoajarticles:oai:doaj.org/article:bef3d3e38f014df3a8412a55b275c8ba 2023-05-15T15:17:31+02:00 Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms Tu Zhijian Qi Yumin Simard Frédéric Mancini Emiliano Santolamazza Federica della Torre Alessandra 2008-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-163 https://doaj.org/article/bef3d3e38f014df3a8412a55b275c8ba EN eng BMC http://www.malariajournal.com/content/7/1/163 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-163 1475-2875 https://doaj.org/article/bef3d3e38f014df3a8412a55b275c8ba Malaria Journal, Vol 7, Iss 1, p 163 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-163 2022-12-31T08:43:35Z Abstract Background SINEs (Short INterspersed Elements) are homoplasy-free and co-dominant genetic markers which are considered to represent useful tools for population genetic studies, and could help clarifying the speciation processes ongoing within the major malaria vector in Africa, Anopheles gambiae s.s. Here, we report the results of the analysis of the insertion polymorphism of a nearly 200 bp-long SINE ( SINE200 ) within genome areas of high differentiation (i.e. "speciation islands") of M and S A. gambiae molecular forms. Methods A SINE -PCR approach was carried out on thirteen SINE200 insertions in M and S females collected along the whole range of distribution of A. gambiae s.s. in sub-Saharan Africa. Ten specimens each for Anopheles arabiensis , Anopheles melas, Anopheles quadriannulatus A and 15 M/S hybrids from laboratory crosses were also analysed. Results Eight loci were successfully amplified and were found to be specific for A. gambiae s.s.: 5 on 2L chromosome and one on X chromosome resulted monomorphic, while two loci positioned respectively on 2R (i.e. S200 2R12D) and X (i.e. S200 X6.1) chromosomes were found to be polymorphic. S200 2R12D was homozygote for the insertion in most S-form samples, while intermediate levels of polymorphism were shown in M-form, resulting in an overall high degree of genetic differentiation between molecular forms (Fst = 0.46 p < 0.001) and within M-form (Fst = 0.46 p < 0.001). The insertion of S200 X6.1 was found to be fixed in all M- and absent in all S-specimens. This led to develop a novel easy-to-use PCR approach to straightforwardly identify A. gambiae molecular forms. This novel approach allows to overcome the constraints associated with markers on the rDNA region commonly used for M and S identification. In fact, it is based on a single copy and irreversible SINE200 insertion and, thus, is not subjected to peculiar evolutionary patterns affecting rDNA markers, e.g. incomplete homogenization of the arrays through concerted evolution and/or mixtures ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 7 1 163 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Tu Zhijian Qi Yumin Simard Frédéric Mancini Emiliano Santolamazza Federica della Torre Alessandra Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background SINEs (Short INterspersed Elements) are homoplasy-free and co-dominant genetic markers which are considered to represent useful tools for population genetic studies, and could help clarifying the speciation processes ongoing within the major malaria vector in Africa, Anopheles gambiae s.s. Here, we report the results of the analysis of the insertion polymorphism of a nearly 200 bp-long SINE ( SINE200 ) within genome areas of high differentiation (i.e. "speciation islands") of M and S A. gambiae molecular forms. Methods A SINE -PCR approach was carried out on thirteen SINE200 insertions in M and S females collected along the whole range of distribution of A. gambiae s.s. in sub-Saharan Africa. Ten specimens each for Anopheles arabiensis , Anopheles melas, Anopheles quadriannulatus A and 15 M/S hybrids from laboratory crosses were also analysed. Results Eight loci were successfully amplified and were found to be specific for A. gambiae s.s.: 5 on 2L chromosome and one on X chromosome resulted monomorphic, while two loci positioned respectively on 2R (i.e. S200 2R12D) and X (i.e. S200 X6.1) chromosomes were found to be polymorphic. S200 2R12D was homozygote for the insertion in most S-form samples, while intermediate levels of polymorphism were shown in M-form, resulting in an overall high degree of genetic differentiation between molecular forms (Fst = 0.46 p < 0.001) and within M-form (Fst = 0.46 p < 0.001). The insertion of S200 X6.1 was found to be fixed in all M- and absent in all S-specimens. This led to develop a novel easy-to-use PCR approach to straightforwardly identify A. gambiae molecular forms. This novel approach allows to overcome the constraints associated with markers on the rDNA region commonly used for M and S identification. In fact, it is based on a single copy and irreversible SINE200 insertion and, thus, is not subjected to peculiar evolutionary patterns affecting rDNA markers, e.g. incomplete homogenization of the arrays through concerted evolution and/or mixtures ... |
format |
Article in Journal/Newspaper |
author |
Tu Zhijian Qi Yumin Simard Frédéric Mancini Emiliano Santolamazza Federica della Torre Alessandra |
author_facet |
Tu Zhijian Qi Yumin Simard Frédéric Mancini Emiliano Santolamazza Federica della Torre Alessandra |
author_sort |
Tu Zhijian |
title |
Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms |
title_short |
Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms |
title_full |
Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms |
title_fullStr |
Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms |
title_full_unstemmed |
Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms |
title_sort |
insertion polymorphisms of sine200 retrotransposons within speciation islands of anopheles gambiae molecular forms |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-163 https://doaj.org/article/bef3d3e38f014df3a8412a55b275c8ba |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 163 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/163 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-163 1475-2875 https://doaj.org/article/bef3d3e38f014df3a8412a55b275c8ba |
op_doi |
https://doi.org/10.1186/1475-2875-7-163 |
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Malaria Journal |
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7 |
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163 |
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