Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems
Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stabl...
Published in: | Hydrology and Earth System Sciences |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Copernicus Publications
2022
|
Subjects: | |
Online Access: | https://doi.org/10.5194/hess-26-3573-2022 https://doaj.org/article/bee9d298bb8a4596a425695e7765be2d |
id |
ftdoajarticles:oai:doaj.org/article:bee9d298bb8a4596a425695e7765be2d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:bee9d298bb8a4596a425695e7765be2d 2023-05-15T17:45:11+02:00 Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems R.-K. Magh B. Gralher B. Herbstritt A. Kübert H. Lim T. Lundmark J. Marshall 2022-07-01T00:00:00Z https://doi.org/10.5194/hess-26-3573-2022 https://doaj.org/article/bee9d298bb8a4596a425695e7765be2d EN eng Copernicus Publications https://hess.copernicus.org/articles/26/3573/2022/hess-26-3573-2022.pdf https://doaj.org/toc/1027-5606 https://doaj.org/toc/1607-7938 doi:10.5194/hess-26-3573-2022 1027-5606 1607-7938 https://doaj.org/article/bee9d298bb8a4596a425695e7765be2d Hydrology and Earth System Sciences, Vol 26, Pp 3573-3587 (2022) Technology T Environmental technology. Sanitary engineering TD1-1066 Geography. Anthropology. Recreation G Environmental sciences GE1-350 article 2022 ftdoajarticles https://doi.org/10.5194/hess-26-3573-2022 2022-12-31T01:53:30Z Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stable isotope composition of plant or soil water, on-site and in real-time. However, current in situ systems require the presence of an instrument in the field. Here we tested, first in the lab and then in the field, a method for equilibrating, collecting, storing, and finally analysing water vapour for its isotopic composition that does not require an instrument in the field. We developed a vapour storage vial system (VSVS) that relies on in situ sampling into crimp neck vials with a double-coated cap using a pump and a flow metre powered through a small battery and measuring the samples in a laboratory. All components are inexpensive and commercially available. We tested the system's ability to store the isotopic composition of its contents by sampling a range of water vapour of known isotopic compositions (from −95 ‰ to +1700 ‰ for δ 2 H ) and measuring the isotopic composition after different storage periods. Samples for the field trial were taken in a boreal forest in northern Sweden. The isotopic composition was maintained to within 0.6 ‰ to 4.4 ‰ for δ 2 H and 0.6 ‰ to 0.8 ‰ for δ 18 O for natural-abundance samples. Although 2 H -enriched samples showed greater uncertainty, they were sufficient to quantify label amounts. We detected a small change in the isotopic composition of the sample after a long storage period, but it was correctable by linear regression models. We observed the same trend for the samples obtained in the field trial for δ 18 O but observed higher variation in δ 2 H than in the lab trial. Our method combines the best of two worlds, sampling many trees in situ while measuring at high precision in the laboratory. This provides the ecohydrology community with a tool that is not only cost efficient ... Article in Journal/Newspaper Northern Sweden Directory of Open Access Journals: DOAJ Articles Hydrology and Earth System Sciences 26 13 3573 3587 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Technology T Environmental technology. Sanitary engineering TD1-1066 Geography. Anthropology. Recreation G Environmental sciences GE1-350 |
spellingShingle |
Technology T Environmental technology. Sanitary engineering TD1-1066 Geography. Anthropology. Recreation G Environmental sciences GE1-350 R.-K. Magh B. Gralher B. Herbstritt A. Kübert H. Lim T. Lundmark J. Marshall Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems |
topic_facet |
Technology T Environmental technology. Sanitary engineering TD1-1066 Geography. Anthropology. Recreation G Environmental sciences GE1-350 |
description |
Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stable isotope composition of plant or soil water, on-site and in real-time. However, current in situ systems require the presence of an instrument in the field. Here we tested, first in the lab and then in the field, a method for equilibrating, collecting, storing, and finally analysing water vapour for its isotopic composition that does not require an instrument in the field. We developed a vapour storage vial system (VSVS) that relies on in situ sampling into crimp neck vials with a double-coated cap using a pump and a flow metre powered through a small battery and measuring the samples in a laboratory. All components are inexpensive and commercially available. We tested the system's ability to store the isotopic composition of its contents by sampling a range of water vapour of known isotopic compositions (from −95 ‰ to +1700 ‰ for δ 2 H ) and measuring the isotopic composition after different storage periods. Samples for the field trial were taken in a boreal forest in northern Sweden. The isotopic composition was maintained to within 0.6 ‰ to 4.4 ‰ for δ 2 H and 0.6 ‰ to 0.8 ‰ for δ 18 O for natural-abundance samples. Although 2 H -enriched samples showed greater uncertainty, they were sufficient to quantify label amounts. We detected a small change in the isotopic composition of the sample after a long storage period, but it was correctable by linear regression models. We observed the same trend for the samples obtained in the field trial for δ 18 O but observed higher variation in δ 2 H than in the lab trial. Our method combines the best of two worlds, sampling many trees in situ while measuring at high precision in the laboratory. This provides the ecohydrology community with a tool that is not only cost efficient ... |
format |
Article in Journal/Newspaper |
author |
R.-K. Magh B. Gralher B. Herbstritt A. Kübert H. Lim T. Lundmark J. Marshall |
author_facet |
R.-K. Magh B. Gralher B. Herbstritt A. Kübert H. Lim T. Lundmark J. Marshall |
author_sort |
R.-K. Magh |
title |
Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems |
title_short |
Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems |
title_full |
Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems |
title_fullStr |
Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems |
title_full_unstemmed |
Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems |
title_sort |
technical note: conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems |
publisher |
Copernicus Publications |
publishDate |
2022 |
url |
https://doi.org/10.5194/hess-26-3573-2022 https://doaj.org/article/bee9d298bb8a4596a425695e7765be2d |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Hydrology and Earth System Sciences, Vol 26, Pp 3573-3587 (2022) |
op_relation |
https://hess.copernicus.org/articles/26/3573/2022/hess-26-3573-2022.pdf https://doaj.org/toc/1027-5606 https://doaj.org/toc/1607-7938 doi:10.5194/hess-26-3573-2022 1027-5606 1607-7938 https://doaj.org/article/bee9d298bb8a4596a425695e7765be2d |
op_doi |
https://doi.org/10.5194/hess-26-3573-2022 |
container_title |
Hydrology and Earth System Sciences |
container_volume |
26 |
container_issue |
13 |
container_start_page |
3573 |
op_container_end_page |
3587 |
_version_ |
1766147997066854400 |