The development of multiplex STR panels for the identification of bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos)
The ability to reliably genotype individual bald eagle (Haliaeetus leucocephalus) and golden eagle (Aquila chrysaetos) samples has proven to be a valuable forensic tool for investigations of illegal trafficking of eagle feathers and body parts. Twenty STR loci were assembled into three optimized mul...
Published in: | Forensic Science International: Animals and Environments |
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Online Access: | https://doi.org/10.1016/j.fsiae.2022.100062 https://doaj.org/article/bed2da049c4e474985e971835d4fb8db |
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ftdoajarticles:oai:doaj.org/article:bed2da049c4e474985e971835d4fb8db 2023-07-16T04:01:18+02:00 The development of multiplex STR panels for the identification of bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) Darren J. Wostenberg Mary K. Burnham-Curtis 2023-12-01T00:00:00Z https://doi.org/10.1016/j.fsiae.2022.100062 https://doaj.org/article/bed2da049c4e474985e971835d4fb8db EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2666937422000221 https://doaj.org/toc/2666-9374 2666-9374 doi:10.1016/j.fsiae.2022.100062 https://doaj.org/article/bed2da049c4e474985e971835d4fb8db Forensic Science International: Animals and Environments, Vol 3, Iss , Pp 100062- (2023) Bald eagle Golden eagle Haliaeetus leucocephalus Aquila chrysaetos STR multiplex Species identification Ecology QH540-549.5 Veterinary medicine SF600-1100 article 2023 ftdoajarticles https://doi.org/10.1016/j.fsiae.2022.100062 2023-06-25T00:35:39Z The ability to reliably genotype individual bald eagle (Haliaeetus leucocephalus) and golden eagle (Aquila chrysaetos) samples has proven to be a valuable forensic tool for investigations of illegal trafficking of eagle feathers and body parts. Twenty STR loci were assembled into three optimized multiplex STR panels. The three STR multiplex PCRs include loci ranging from dinucleotide to pentanucleotide motifs, and both monomorphic and polymorphic amplification. The number of alleles for bald eagles ranged 1–5 (14 polymorphic loci), and the number of alleles for golden eagles ranged from 1 to 11 (16 polymorphic loci). Bald and golden eagles shared alleles at 5 loci, the other 15 loci yielding alleles characteristic of each species. These multiplex panels will be useful in wildlife law enforcement investigations in which eagle feathers and body parts can be identified to species and individuals matched to link a crime scene and eagle victim to a suspect. Article in Journal/Newspaper Aquila chrysaetos golden eagle Directory of Open Access Journals: DOAJ Articles Forensic Science International: Animals and Environments 3 100062 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bald eagle Golden eagle Haliaeetus leucocephalus Aquila chrysaetos STR multiplex Species identification Ecology QH540-549.5 Veterinary medicine SF600-1100 |
spellingShingle |
Bald eagle Golden eagle Haliaeetus leucocephalus Aquila chrysaetos STR multiplex Species identification Ecology QH540-549.5 Veterinary medicine SF600-1100 Darren J. Wostenberg Mary K. Burnham-Curtis The development of multiplex STR panels for the identification of bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) |
topic_facet |
Bald eagle Golden eagle Haliaeetus leucocephalus Aquila chrysaetos STR multiplex Species identification Ecology QH540-549.5 Veterinary medicine SF600-1100 |
description |
The ability to reliably genotype individual bald eagle (Haliaeetus leucocephalus) and golden eagle (Aquila chrysaetos) samples has proven to be a valuable forensic tool for investigations of illegal trafficking of eagle feathers and body parts. Twenty STR loci were assembled into three optimized multiplex STR panels. The three STR multiplex PCRs include loci ranging from dinucleotide to pentanucleotide motifs, and both monomorphic and polymorphic amplification. The number of alleles for bald eagles ranged 1–5 (14 polymorphic loci), and the number of alleles for golden eagles ranged from 1 to 11 (16 polymorphic loci). Bald and golden eagles shared alleles at 5 loci, the other 15 loci yielding alleles characteristic of each species. These multiplex panels will be useful in wildlife law enforcement investigations in which eagle feathers and body parts can be identified to species and individuals matched to link a crime scene and eagle victim to a suspect. |
format |
Article in Journal/Newspaper |
author |
Darren J. Wostenberg Mary K. Burnham-Curtis |
author_facet |
Darren J. Wostenberg Mary K. Burnham-Curtis |
author_sort |
Darren J. Wostenberg |
title |
The development of multiplex STR panels for the identification of bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) |
title_short |
The development of multiplex STR panels for the identification of bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) |
title_full |
The development of multiplex STR panels for the identification of bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) |
title_fullStr |
The development of multiplex STR panels for the identification of bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) |
title_full_unstemmed |
The development of multiplex STR panels for the identification of bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) |
title_sort |
development of multiplex str panels for the identification of bald eagles (haliaeetus leucocephalus) and golden eagles (aquila chrysaetos) |
publisher |
Elsevier |
publishDate |
2023 |
url |
https://doi.org/10.1016/j.fsiae.2022.100062 https://doaj.org/article/bed2da049c4e474985e971835d4fb8db |
genre |
Aquila chrysaetos golden eagle |
genre_facet |
Aquila chrysaetos golden eagle |
op_source |
Forensic Science International: Animals and Environments, Vol 3, Iss , Pp 100062- (2023) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2666937422000221 https://doaj.org/toc/2666-9374 2666-9374 doi:10.1016/j.fsiae.2022.100062 https://doaj.org/article/bed2da049c4e474985e971835d4fb8db |
op_doi |
https://doi.org/10.1016/j.fsiae.2022.100062 |
container_title |
Forensic Science International: Animals and Environments |
container_volume |
3 |
container_start_page |
100062 |
_version_ |
1771550977374027776 |