Biotransformation of vanillin into vanillyl alcohol by a novel strain of Cystobasidium laryngis isolated from decaying wood
Abstract Vanillin is an aromatic aldehyde found as a component of lignocellulosic material, and in the cured pods of orchidaceae plants. Like other phenolic substances, vanillin has antimicrobial activity and can be extracted from lignin either by a thermo-chemical process or through microbial degra...
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Online Access: | https://doi.org/10.1186/s13568-018-0666-4 https://doaj.org/article/beb7e1b11f1e40a1b928770e83989c8c |
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ftdoajarticles:oai:doaj.org/article:beb7e1b11f1e40a1b928770e83989c8c 2023-05-15T16:10:53+02:00 Biotransformation of vanillin into vanillyl alcohol by a novel strain of Cystobasidium laryngis isolated from decaying wood Jonas Rönnander Joel Ljunggren Erik Hedenström Sandra Ann Ingela Wright 2018-08-01T00:00:00Z https://doi.org/10.1186/s13568-018-0666-4 https://doaj.org/article/beb7e1b11f1e40a1b928770e83989c8c EN eng SpringerOpen http://link.springer.com/article/10.1186/s13568-018-0666-4 https://doaj.org/toc/2191-0855 doi:10.1186/s13568-018-0666-4 2191-0855 https://doaj.org/article/beb7e1b11f1e40a1b928770e83989c8c AMB Express, Vol 8, Iss 1, Pp 1-8 (2018) Vanillin Cystobasidium Bioconversion Biodegradation Cystobasidiomycetes Rhodotorula Biotechnology TP248.13-248.65 Microbiology QR1-502 article 2018 ftdoajarticles https://doi.org/10.1186/s13568-018-0666-4 2022-12-30T22:14:06Z Abstract Vanillin is an aromatic aldehyde found as a component of lignocellulosic material, and in the cured pods of orchidaceae plants. Like other phenolic substances, vanillin has antimicrobial activity and can be extracted from lignin either by a thermo-chemical process or through microbial degradation. Vanillin, can serve as a model monomer in biodegradation studies of lignin. In the present study, a yeast isolated from decaying wood on the Faroe Islands, was identified as Cystobasidium laryngis strain FMYD002, based on internal transcribed spacer sequence analysis. It demonstrated the ability to convert vanillin to vanillyl alcohol, as detected by ultra-high performance liquid chromatography–quadrupole-time-of-flight. Structural analysis of vanillyl alcohol was carried out by using gas chromatography–mass spectrometry and 1H NMR spectroscopy, and further verified by synthesis. The reduction of vanillin to vanillyl alcohol has been documented for only a few species of fungi. However, to our knowledge, this biotransformation has not yet been reported for basidiomycetous yeast species, nor for any representative of the subphylum Pucciniomycotina. The biotransformation capability of the present strain might prove useful in the industrial utilisation of lignocellulosic residues. Article in Journal/Newspaper Faroe Islands Directory of Open Access Journals: DOAJ Articles Faroe Islands AMB Express 8 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Vanillin Cystobasidium Bioconversion Biodegradation Cystobasidiomycetes Rhodotorula Biotechnology TP248.13-248.65 Microbiology QR1-502 |
spellingShingle |
Vanillin Cystobasidium Bioconversion Biodegradation Cystobasidiomycetes Rhodotorula Biotechnology TP248.13-248.65 Microbiology QR1-502 Jonas Rönnander Joel Ljunggren Erik Hedenström Sandra Ann Ingela Wright Biotransformation of vanillin into vanillyl alcohol by a novel strain of Cystobasidium laryngis isolated from decaying wood |
topic_facet |
Vanillin Cystobasidium Bioconversion Biodegradation Cystobasidiomycetes Rhodotorula Biotechnology TP248.13-248.65 Microbiology QR1-502 |
description |
Abstract Vanillin is an aromatic aldehyde found as a component of lignocellulosic material, and in the cured pods of orchidaceae plants. Like other phenolic substances, vanillin has antimicrobial activity and can be extracted from lignin either by a thermo-chemical process or through microbial degradation. Vanillin, can serve as a model monomer in biodegradation studies of lignin. In the present study, a yeast isolated from decaying wood on the Faroe Islands, was identified as Cystobasidium laryngis strain FMYD002, based on internal transcribed spacer sequence analysis. It demonstrated the ability to convert vanillin to vanillyl alcohol, as detected by ultra-high performance liquid chromatography–quadrupole-time-of-flight. Structural analysis of vanillyl alcohol was carried out by using gas chromatography–mass spectrometry and 1H NMR spectroscopy, and further verified by synthesis. The reduction of vanillin to vanillyl alcohol has been documented for only a few species of fungi. However, to our knowledge, this biotransformation has not yet been reported for basidiomycetous yeast species, nor for any representative of the subphylum Pucciniomycotina. The biotransformation capability of the present strain might prove useful in the industrial utilisation of lignocellulosic residues. |
format |
Article in Journal/Newspaper |
author |
Jonas Rönnander Joel Ljunggren Erik Hedenström Sandra Ann Ingela Wright |
author_facet |
Jonas Rönnander Joel Ljunggren Erik Hedenström Sandra Ann Ingela Wright |
author_sort |
Jonas Rönnander |
title |
Biotransformation of vanillin into vanillyl alcohol by a novel strain of Cystobasidium laryngis isolated from decaying wood |
title_short |
Biotransformation of vanillin into vanillyl alcohol by a novel strain of Cystobasidium laryngis isolated from decaying wood |
title_full |
Biotransformation of vanillin into vanillyl alcohol by a novel strain of Cystobasidium laryngis isolated from decaying wood |
title_fullStr |
Biotransformation of vanillin into vanillyl alcohol by a novel strain of Cystobasidium laryngis isolated from decaying wood |
title_full_unstemmed |
Biotransformation of vanillin into vanillyl alcohol by a novel strain of Cystobasidium laryngis isolated from decaying wood |
title_sort |
biotransformation of vanillin into vanillyl alcohol by a novel strain of cystobasidium laryngis isolated from decaying wood |
publisher |
SpringerOpen |
publishDate |
2018 |
url |
https://doi.org/10.1186/s13568-018-0666-4 https://doaj.org/article/beb7e1b11f1e40a1b928770e83989c8c |
geographic |
Faroe Islands |
geographic_facet |
Faroe Islands |
genre |
Faroe Islands |
genre_facet |
Faroe Islands |
op_source |
AMB Express, Vol 8, Iss 1, Pp 1-8 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s13568-018-0666-4 https://doaj.org/toc/2191-0855 doi:10.1186/s13568-018-0666-4 2191-0855 https://doaj.org/article/beb7e1b11f1e40a1b928770e83989c8c |
op_doi |
https://doi.org/10.1186/s13568-018-0666-4 |
container_title |
AMB Express |
container_volume |
8 |
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1 |
_version_ |
1765996017024499712 |