Diversity analysis of MSP1 identifies conserved epitope organization in block 2 amidst high sequence variability in Indian Plasmodium falciparum isolates
Abstract Background Despite its immunogenicity, the polymorphic nature of merozoite surface protein 1, an important vaccine candidate for Plasmodium falciparum malaria, remains a concern. This study analyses the impact of genetic variability and parasite population structure on epitope organization...
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ftdoajarticles:oai:doaj.org/article:be801f5733fe4a75a4a9ce54d29a0fa1 2023-05-15T15:16:23+02:00 Diversity analysis of MSP1 identifies conserved epitope organization in block 2 amidst high sequence variability in Indian Plasmodium falciparum isolates Sharmistha Ghoshal Pragya Gajendra Sumana Datta Kanjilal Mitashree Mitra Sanghamitra Sengupta 2018-12-01T00:00:00Z https://doi.org/10.1186/s12936-018-2592-y https://doaj.org/article/be801f5733fe4a75a4a9ce54d29a0fa1 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2592-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2592-y 1475-2875 https://doaj.org/article/be801f5733fe4a75a4a9ce54d29a0fa1 Malaria Journal, Vol 17, Iss 1, Pp 1-14 (2018) Plasmodium falciparum MSP1 Genetic diversity Multiclonal infection Epitope organization India Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2592-y 2022-12-31T09:04:47Z Abstract Background Despite its immunogenicity, the polymorphic nature of merozoite surface protein 1, an important vaccine candidate for Plasmodium falciparum malaria, remains a concern. This study analyses the impact of genetic variability and parasite population structure on epitope organization of different MSP1 segments. Methods Altogether 98 blood samples collected from P. falciparum infected mild and severe malaria patients of Chhattisgarh and West Bengal were used to sequence regions encoding block 2 and MSP1-19 of msp1. Sequences were analysed using MEGA7, DnaSPv5, Arlequin3.5 and BepiPred. Results All three major MSP1 block 2 allele families namely K1, MAD20 and RO33 were detected in the samples and they together resulted in 41 indel variants. Chhattisgarh samples displayed an average MOI of 2.07 ± 1.59 which was higher in mild malaria and in age group < 18 years. Ultra-structure of block 2 alleles revealed that mutation and repeat expansion were two major mechanisms responsible for allelic variability of K1 and MAD20. Regions flanking block 2 were highly variable in Chhattisgarh with average mismatch differences (k) ranging from 1.198 to 5.156 for three families. In contrast, region encompassing MSP1-19 exhibited limited heterogeneity (kChhattisgarh = 1.45, kWest Bengal = 1.363). Of the 50 possible B cell linear epitopes predicted from block 2 variants, 94.9% (131 of 138) of the parasites could be represented by three conserved antigens. Conclusions Present data indicates that natural selection and transmission intensity jointly play a role in controlling allelic diversity of MSP1 in Indian parasite isolates. Despite remarkable genetic variability, a limited number of predominant and conserved epitopes are present in Indian parasite isolates reinstating the importance of MSP1 as a promising malaria vaccine candidate. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indel’ ENVELOPE(35.282,35.282,66.963,66.963) Indian Malaria Journal 17 1 |
institution |
Open Polar |
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op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum MSP1 Genetic diversity Multiclonal infection Epitope organization India Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum MSP1 Genetic diversity Multiclonal infection Epitope organization India Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sharmistha Ghoshal Pragya Gajendra Sumana Datta Kanjilal Mitashree Mitra Sanghamitra Sengupta Diversity analysis of MSP1 identifies conserved epitope organization in block 2 amidst high sequence variability in Indian Plasmodium falciparum isolates |
topic_facet |
Plasmodium falciparum MSP1 Genetic diversity Multiclonal infection Epitope organization India Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Despite its immunogenicity, the polymorphic nature of merozoite surface protein 1, an important vaccine candidate for Plasmodium falciparum malaria, remains a concern. This study analyses the impact of genetic variability and parasite population structure on epitope organization of different MSP1 segments. Methods Altogether 98 blood samples collected from P. falciparum infected mild and severe malaria patients of Chhattisgarh and West Bengal were used to sequence regions encoding block 2 and MSP1-19 of msp1. Sequences were analysed using MEGA7, DnaSPv5, Arlequin3.5 and BepiPred. Results All three major MSP1 block 2 allele families namely K1, MAD20 and RO33 were detected in the samples and they together resulted in 41 indel variants. Chhattisgarh samples displayed an average MOI of 2.07 ± 1.59 which was higher in mild malaria and in age group < 18 years. Ultra-structure of block 2 alleles revealed that mutation and repeat expansion were two major mechanisms responsible for allelic variability of K1 and MAD20. Regions flanking block 2 were highly variable in Chhattisgarh with average mismatch differences (k) ranging from 1.198 to 5.156 for three families. In contrast, region encompassing MSP1-19 exhibited limited heterogeneity (kChhattisgarh = 1.45, kWest Bengal = 1.363). Of the 50 possible B cell linear epitopes predicted from block 2 variants, 94.9% (131 of 138) of the parasites could be represented by three conserved antigens. Conclusions Present data indicates that natural selection and transmission intensity jointly play a role in controlling allelic diversity of MSP1 in Indian parasite isolates. Despite remarkable genetic variability, a limited number of predominant and conserved epitopes are present in Indian parasite isolates reinstating the importance of MSP1 as a promising malaria vaccine candidate. |
format |
Article in Journal/Newspaper |
author |
Sharmistha Ghoshal Pragya Gajendra Sumana Datta Kanjilal Mitashree Mitra Sanghamitra Sengupta |
author_facet |
Sharmistha Ghoshal Pragya Gajendra Sumana Datta Kanjilal Mitashree Mitra Sanghamitra Sengupta |
author_sort |
Sharmistha Ghoshal |
title |
Diversity analysis of MSP1 identifies conserved epitope organization in block 2 amidst high sequence variability in Indian Plasmodium falciparum isolates |
title_short |
Diversity analysis of MSP1 identifies conserved epitope organization in block 2 amidst high sequence variability in Indian Plasmodium falciparum isolates |
title_full |
Diversity analysis of MSP1 identifies conserved epitope organization in block 2 amidst high sequence variability in Indian Plasmodium falciparum isolates |
title_fullStr |
Diversity analysis of MSP1 identifies conserved epitope organization in block 2 amidst high sequence variability in Indian Plasmodium falciparum isolates |
title_full_unstemmed |
Diversity analysis of MSP1 identifies conserved epitope organization in block 2 amidst high sequence variability in Indian Plasmodium falciparum isolates |
title_sort |
diversity analysis of msp1 identifies conserved epitope organization in block 2 amidst high sequence variability in indian plasmodium falciparum isolates |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2592-y https://doaj.org/article/be801f5733fe4a75a4a9ce54d29a0fa1 |
long_lat |
ENVELOPE(35.282,35.282,66.963,66.963) |
geographic |
Arctic Indel’ Indian |
geographic_facet |
Arctic Indel’ Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-14 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2592-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2592-y 1475-2875 https://doaj.org/article/be801f5733fe4a75a4a9ce54d29a0fa1 |
op_doi |
https://doi.org/10.1186/s12936-018-2592-y |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
_version_ |
1766346671213510656 |