Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation
The bacterial community structures in High-Arctic snow over sea ice and an ice-covered freshwater lake were examined by pyrosequencing of 16S rRNA genes and 16S rRNA gene sequencing of cultivated isolates. Both the pyrosequence and cultivation data indicated that the phylogenetic composition of the...
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Norwegian Polar Institute
2013
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ftdoajarticles:oai:doaj.org/article:be74effb95004bdb8f062e343e5731c7 2023-05-15T14:53:31+02:00 Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation Annette K. Møller Ditte A. Søborg Waleed Abu Al-Soud Søren J. Sørensen Niels Kroer 2013-04-01T00:00:00Z https://doi.org/10.3402/polar.v32i0.17390 https://doaj.org/article/be74effb95004bdb8f062e343e5731c7 EN eng Norwegian Polar Institute http://www.polarresearch.net/index.php/polar/article/download/17390/pdf_1 https://doaj.org/toc/0800-0395 https://doaj.org/toc/1751-8369 doi:10.3402/polar.v32i0.17390 0800-0395 1751-8369 https://doaj.org/article/be74effb95004bdb8f062e343e5731c7 Polar Research, Vol 32, Iss 0, Pp 1-11 (2013) Taxonomic diversity microbial assemblages bacterial density DOC Environmental sciences GE1-350 Oceanography GC1-1581 article 2013 ftdoajarticles https://doi.org/10.3402/polar.v32i0.17390 2022-12-31T09:10:11Z The bacterial community structures in High-Arctic snow over sea ice and an ice-covered freshwater lake were examined by pyrosequencing of 16S rRNA genes and 16S rRNA gene sequencing of cultivated isolates. Both the pyrosequence and cultivation data indicated that the phylogenetic composition of the microbial assemblages was different within the snow layers and between snow and freshwater. The highest diversity was seen in snow. In the middle and top snow layers, Proteobacteria, Bacteroidetes and Cyanobacteria dominated, although Actinobacteria and Firmicutes were relatively abundant also. High numbers of chloroplasts were also observed. In the deepest snow layer, large percentages of Firmicutes and Fusobacteria were seen. In freshwater, Bacteroidetes, Actinobacteria and Verrucomicrobia were the most abundant phyla while relatively few Proteobacteria and Cyanobacteria were present. Possibly, light intensity controlled the distribution of the Cyanobacteria and algae in the snow while carbon and nitrogen fixed by these autotrophs in turn fed the heterotrophic bacteria. In the lake, a probable lower light input relative to snow resulted in low numbers of Cyanobacteria and chloroplasts and, hence, limited input of organic carbon and nitrogen to the heterotrophic bacteria. Thus, differences in the physicochemical conditions may play an important role in the processes leading to distinctive bacterial community structures in High-Arctic snow and freshwater. Article in Journal/Newspaper Arctic Polar Research Sea ice Directory of Open Access Journals: DOAJ Articles Arctic Polar Research 32 1 17390 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Taxonomic diversity microbial assemblages bacterial density DOC Environmental sciences GE1-350 Oceanography GC1-1581 |
spellingShingle |
Taxonomic diversity microbial assemblages bacterial density DOC Environmental sciences GE1-350 Oceanography GC1-1581 Annette K. Møller Ditte A. Søborg Waleed Abu Al-Soud Søren J. Sørensen Niels Kroer Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
topic_facet |
Taxonomic diversity microbial assemblages bacterial density DOC Environmental sciences GE1-350 Oceanography GC1-1581 |
description |
The bacterial community structures in High-Arctic snow over sea ice and an ice-covered freshwater lake were examined by pyrosequencing of 16S rRNA genes and 16S rRNA gene sequencing of cultivated isolates. Both the pyrosequence and cultivation data indicated that the phylogenetic composition of the microbial assemblages was different within the snow layers and between snow and freshwater. The highest diversity was seen in snow. In the middle and top snow layers, Proteobacteria, Bacteroidetes and Cyanobacteria dominated, although Actinobacteria and Firmicutes were relatively abundant also. High numbers of chloroplasts were also observed. In the deepest snow layer, large percentages of Firmicutes and Fusobacteria were seen. In freshwater, Bacteroidetes, Actinobacteria and Verrucomicrobia were the most abundant phyla while relatively few Proteobacteria and Cyanobacteria were present. Possibly, light intensity controlled the distribution of the Cyanobacteria and algae in the snow while carbon and nitrogen fixed by these autotrophs in turn fed the heterotrophic bacteria. In the lake, a probable lower light input relative to snow resulted in low numbers of Cyanobacteria and chloroplasts and, hence, limited input of organic carbon and nitrogen to the heterotrophic bacteria. Thus, differences in the physicochemical conditions may play an important role in the processes leading to distinctive bacterial community structures in High-Arctic snow and freshwater. |
format |
Article in Journal/Newspaper |
author |
Annette K. Møller Ditte A. Søborg Waleed Abu Al-Soud Søren J. Sørensen Niels Kroer |
author_facet |
Annette K. Møller Ditte A. Søborg Waleed Abu Al-Soud Søren J. Sørensen Niels Kroer |
author_sort |
Annette K. Møller |
title |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_short |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_full |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_fullStr |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_full_unstemmed |
Bacterial community structure in High-Arctic snow and freshwater as revealed by pyrosequencing of 16S rRNA genes and cultivation |
title_sort |
bacterial community structure in high-arctic snow and freshwater as revealed by pyrosequencing of 16s rrna genes and cultivation |
publisher |
Norwegian Polar Institute |
publishDate |
2013 |
url |
https://doi.org/10.3402/polar.v32i0.17390 https://doaj.org/article/be74effb95004bdb8f062e343e5731c7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Polar Research Sea ice |
genre_facet |
Arctic Polar Research Sea ice |
op_source |
Polar Research, Vol 32, Iss 0, Pp 1-11 (2013) |
op_relation |
http://www.polarresearch.net/index.php/polar/article/download/17390/pdf_1 https://doaj.org/toc/0800-0395 https://doaj.org/toc/1751-8369 doi:10.3402/polar.v32i0.17390 0800-0395 1751-8369 https://doaj.org/article/be74effb95004bdb8f062e343e5731c7 |
op_doi |
https://doi.org/10.3402/polar.v32i0.17390 |
container_title |
Polar Research |
container_volume |
32 |
container_issue |
1 |
container_start_page |
17390 |
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1766325108032405504 |