A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths.
The Kato-Katz thick smear (Kato-Katz) is the diagnostic method recommended for monitoring large-scale treatment programs implemented for the control of soil-transmitted helminths (STH) in public health, yet it is difficult to standardize. A promising alternative is the McMaster egg counting method (...
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ftdoajarticles:oai:doaj.org/article:be70e2b61c854fd99858fd92f8939c64 2023-05-15T15:18:00+02:00 A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths. Bruno Levecke Jerzy M Behnke Sitara S R Ajjampur Marco Albonico Shaali M Ame Johannes Charlier Stefan M Geiger Nguyen T V Hoa Romuald I Kamwa Ngassam Andrew C Kotze James S McCarthy Antonio Montresor Maria V Periago Sheela Roy Louis-Albert Tchuem Tchuenté D T C Thach Jozef Vercruysse 2011-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001201 https://doaj.org/article/be70e2b61c854fd99858fd92f8939c64 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3114752?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001201 https://doaj.org/article/be70e2b61c854fd99858fd92f8939c64 PLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1201 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001201 2022-12-31T00:09:04Z The Kato-Katz thick smear (Kato-Katz) is the diagnostic method recommended for monitoring large-scale treatment programs implemented for the control of soil-transmitted helminths (STH) in public health, yet it is difficult to standardize. A promising alternative is the McMaster egg counting method (McMaster), commonly used in veterinary parasitology, but rarely so for the detection of STH in human stool.The Kato-Katz and McMaster methods were compared for the detection of STH in 1,543 subjects resident in five countries across Africa, Asia and South America. The consistency of the performance of both methods in different trials, the validity of the fixed multiplication factor employed in the Kato-Katz method and the accuracy of these methods for estimating 'true' drug efficacies were assessed. The Kato-Katz method detected significantly more Ascaris lumbricoides infections (88.1% vs. 75.6%, p<0.001), whereas the difference in sensitivity between the two methods was non-significant for hookworm (78.3% vs. 72.4%) and Trichuris trichiura (82.6% vs. 80.3%). The sensitivity of the methods varied significantly across trials and magnitude of fecal egg counts (FEC). Quantitative comparison revealed a significant correlation (Rs >0.32) in FEC between both methods, and indicated no significant difference in FEC, except for A. lumbricoides, where the Kato-Katz resulted in significantly higher FEC (14,197 eggs per gram of stool (EPG) vs. 5,982 EPG). For the Kato-Katz, the fixed multiplication factor resulted in significantly higher FEC than the multiplication factor adjusted for mass of feces examined for A. lumbricoides (16,538 EPG vs. 15,396 EPG) and T. trichiura (1,490 EPG vs. 1,363 EPG), but not for hookworm. The McMaster provided more accurate efficacy results (absolute difference to 'true' drug efficacy: 1.7% vs. 4.5%).The McMaster is an alternative method for monitoring large-scale treatment programs. It is a robust (accurate multiplication factor) and accurate (reliable efficacy results) method, which can be ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 6 e1201 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Bruno Levecke Jerzy M Behnke Sitara S R Ajjampur Marco Albonico Shaali M Ame Johannes Charlier Stefan M Geiger Nguyen T V Hoa Romuald I Kamwa Ngassam Andrew C Kotze James S McCarthy Antonio Montresor Maria V Periago Sheela Roy Louis-Albert Tchuem Tchuenté D T C Thach Jozef Vercruysse A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The Kato-Katz thick smear (Kato-Katz) is the diagnostic method recommended for monitoring large-scale treatment programs implemented for the control of soil-transmitted helminths (STH) in public health, yet it is difficult to standardize. A promising alternative is the McMaster egg counting method (McMaster), commonly used in veterinary parasitology, but rarely so for the detection of STH in human stool.The Kato-Katz and McMaster methods were compared for the detection of STH in 1,543 subjects resident in five countries across Africa, Asia and South America. The consistency of the performance of both methods in different trials, the validity of the fixed multiplication factor employed in the Kato-Katz method and the accuracy of these methods for estimating 'true' drug efficacies were assessed. The Kato-Katz method detected significantly more Ascaris lumbricoides infections (88.1% vs. 75.6%, p<0.001), whereas the difference in sensitivity between the two methods was non-significant for hookworm (78.3% vs. 72.4%) and Trichuris trichiura (82.6% vs. 80.3%). The sensitivity of the methods varied significantly across trials and magnitude of fecal egg counts (FEC). Quantitative comparison revealed a significant correlation (Rs >0.32) in FEC between both methods, and indicated no significant difference in FEC, except for A. lumbricoides, where the Kato-Katz resulted in significantly higher FEC (14,197 eggs per gram of stool (EPG) vs. 5,982 EPG). For the Kato-Katz, the fixed multiplication factor resulted in significantly higher FEC than the multiplication factor adjusted for mass of feces examined for A. lumbricoides (16,538 EPG vs. 15,396 EPG) and T. trichiura (1,490 EPG vs. 1,363 EPG), but not for hookworm. The McMaster provided more accurate efficacy results (absolute difference to 'true' drug efficacy: 1.7% vs. 4.5%).The McMaster is an alternative method for monitoring large-scale treatment programs. It is a robust (accurate multiplication factor) and accurate (reliable efficacy results) method, which can be ... |
format |
Article in Journal/Newspaper |
author |
Bruno Levecke Jerzy M Behnke Sitara S R Ajjampur Marco Albonico Shaali M Ame Johannes Charlier Stefan M Geiger Nguyen T V Hoa Romuald I Kamwa Ngassam Andrew C Kotze James S McCarthy Antonio Montresor Maria V Periago Sheela Roy Louis-Albert Tchuem Tchuenté D T C Thach Jozef Vercruysse |
author_facet |
Bruno Levecke Jerzy M Behnke Sitara S R Ajjampur Marco Albonico Shaali M Ame Johannes Charlier Stefan M Geiger Nguyen T V Hoa Romuald I Kamwa Ngassam Andrew C Kotze James S McCarthy Antonio Montresor Maria V Periago Sheela Roy Louis-Albert Tchuem Tchuenté D T C Thach Jozef Vercruysse |
author_sort |
Bruno Levecke |
title |
A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths. |
title_short |
A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths. |
title_full |
A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths. |
title_fullStr |
A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths. |
title_full_unstemmed |
A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths. |
title_sort |
comparison of the sensitivity and fecal egg counts of the mcmaster egg counting and kato-katz thick smear methods for soil-transmitted helminths. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001201 https://doaj.org/article/be70e2b61c854fd99858fd92f8939c64 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1201 (2011) |
op_relation |
http://europepmc.org/articles/PMC3114752?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001201 https://doaj.org/article/be70e2b61c854fd99858fd92f8939c64 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001201 |
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PLoS Neglected Tropical Diseases |
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6 |
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e1201 |
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