Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection.
Tsetse flies (Glossina spp.) transmit parasitic African trypanosomes (Trypanosoma spp.), including Trypanosoma congolense, which causes animal African trypanosomiasis (AAT). AAT detrimentally affects agricultural activities in sub-Saharan Africa and has negative impacts on the livelihood and nutrien...
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ftdoajarticles:oai:doaj.org/article:be54daa9c0dc408480d7b27ada6cb0a5 2023-05-15T15:13:34+02:00 Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection. Erick O Awuoche Brian L Weiss Aurélien Vigneron Paul O Mireji Emre Aksoy Benson Nyambega Geoffrey M Attardo Yineng Wu Michelle O'Neill Grace Murilla Serap Aksoy 2017-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006057 https://doaj.org/article/be54daa9c0dc408480d7b27ada6cb0a5 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5695773?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006057 https://doaj.org/article/be54daa9c0dc408480d7b27ada6cb0a5 PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0006057 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006057 2022-12-31T02:44:12Z Tsetse flies (Glossina spp.) transmit parasitic African trypanosomes (Trypanosoma spp.), including Trypanosoma congolense, which causes animal African trypanosomiasis (AAT). AAT detrimentally affects agricultural activities in sub-Saharan Africa and has negative impacts on the livelihood and nutrient availability for the affected communities. After tsetse ingests an infectious blood meal, T. congolense sequentially colonizes the fly's gut and proboscis (PB) organs before being transmitted to new mammalian hosts during subsequent feedings. Despite the importance of PB in blood feeding and disease transmission, little is known about its molecular composition, function and response to trypanosome infection. To bridge this gap, we used RNA-seq analysis to determine its molecular characteristics and responses to trypanosome infection. By comparing the PB transcriptome to whole head and midgut transcriptomes, we identified 668 PB-enriched transcripts that encoded proteins associated with muscle tissue, organ development, chemosensation and chitin-cuticle structure development. Moreover, transcripts encoding putative mechanoreceptors that monitor blood flow during tsetse feeding and interact with trypanosomes were also expressed in the PB. Microscopic analysis of the PB revealed cellular structures associated with muscles and cells. Infection with T. congolense resulted in increased and decreased expression of 38 and 88 transcripts, respectively. Twelve of these differentially expressed transcripts were PB-enriched. Among the transcripts induced upon infection were those encoding putative proteins associated with cell division function(s), suggesting enhanced tissue renewal, while those suppressed were associated with metabolic processes, extracellular matrix and ATP-binding as well as immunity. These results suggest that PB is a muscular organ with chemosensory and mechanosensory capabilities. The mechanoreceptors may be point of PB-trypanosomes interactions. T. congolense infection resulted in reduced metabolic and ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 11 e0006057 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Erick O Awuoche Brian L Weiss Aurélien Vigneron Paul O Mireji Emre Aksoy Benson Nyambega Geoffrey M Attardo Yineng Wu Michelle O'Neill Grace Murilla Serap Aksoy Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Tsetse flies (Glossina spp.) transmit parasitic African trypanosomes (Trypanosoma spp.), including Trypanosoma congolense, which causes animal African trypanosomiasis (AAT). AAT detrimentally affects agricultural activities in sub-Saharan Africa and has negative impacts on the livelihood and nutrient availability for the affected communities. After tsetse ingests an infectious blood meal, T. congolense sequentially colonizes the fly's gut and proboscis (PB) organs before being transmitted to new mammalian hosts during subsequent feedings. Despite the importance of PB in blood feeding and disease transmission, little is known about its molecular composition, function and response to trypanosome infection. To bridge this gap, we used RNA-seq analysis to determine its molecular characteristics and responses to trypanosome infection. By comparing the PB transcriptome to whole head and midgut transcriptomes, we identified 668 PB-enriched transcripts that encoded proteins associated with muscle tissue, organ development, chemosensation and chitin-cuticle structure development. Moreover, transcripts encoding putative mechanoreceptors that monitor blood flow during tsetse feeding and interact with trypanosomes were also expressed in the PB. Microscopic analysis of the PB revealed cellular structures associated with muscles and cells. Infection with T. congolense resulted in increased and decreased expression of 38 and 88 transcripts, respectively. Twelve of these differentially expressed transcripts were PB-enriched. Among the transcripts induced upon infection were those encoding putative proteins associated with cell division function(s), suggesting enhanced tissue renewal, while those suppressed were associated with metabolic processes, extracellular matrix and ATP-binding as well as immunity. These results suggest that PB is a muscular organ with chemosensory and mechanosensory capabilities. The mechanoreceptors may be point of PB-trypanosomes interactions. T. congolense infection resulted in reduced metabolic and ... |
format |
Article in Journal/Newspaper |
author |
Erick O Awuoche Brian L Weiss Aurélien Vigneron Paul O Mireji Emre Aksoy Benson Nyambega Geoffrey M Attardo Yineng Wu Michelle O'Neill Grace Murilla Serap Aksoy |
author_facet |
Erick O Awuoche Brian L Weiss Aurélien Vigneron Paul O Mireji Emre Aksoy Benson Nyambega Geoffrey M Attardo Yineng Wu Michelle O'Neill Grace Murilla Serap Aksoy |
author_sort |
Erick O Awuoche |
title |
Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection. |
title_short |
Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection. |
title_full |
Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection. |
title_fullStr |
Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection. |
title_full_unstemmed |
Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection. |
title_sort |
molecular characterization of tsetse's proboscis and its response to trypanosoma congolense infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006057 https://doaj.org/article/be54daa9c0dc408480d7b27ada6cb0a5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0006057 (2017) |
op_relation |
http://europepmc.org/articles/PMC5695773?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006057 https://doaj.org/article/be54daa9c0dc408480d7b27ada6cb0a5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006057 |
container_title |
PLOS Neglected Tropical Diseases |
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11 |
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11 |
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e0006057 |
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1766344112183705600 |