The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges
As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of t...
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ftdoajarticles:oai:doaj.org/article:be4978033a1245e98710585ff9926440 2023-05-15T15:01:50+02:00 The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges Jon Thomassen Hestetun Håkon Dahle Steffen Leth Jørgensen Bernt Rydland Olsen Hans Tore Rapp 2016-11-01T00:00:00Z https://doi.org/10.3389/fmicb.2016.01781 https://doaj.org/article/be4978033a1245e98710585ff9926440 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01781/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.01781 https://doaj.org/article/be4978033a1245e98710585ff9926440 Frontiers in Microbiology, Vol 7 (2016) Barbados Porifera methane seep hydrothermal vent isotope Cladorhizidae Microbiology QR1-502 article 2016 ftdoajarticles https://doi.org/10.3389/fmicb.2016.01781 2022-12-30T21:22:34Z As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of the methanotrophic carnivorous sponge Cladorhiza methanophila from a mud volcano-rich area at the Barbados Accretionary Prism (BAP), and five carnivorous species from the Jan Mayen Vent Field (JMVF) at the Arctic Mid-Ocean Ridge. Results from 16S rRNA microbiome data indicate the presence of a diverse assemblage of associated microorganisms in carnivorous sponges mainly from the Gamma- and Alphaproteobacteria, Flavobacteriaceae and Thaumarchaeota. While the abundance of particular groups varied throughout the dataset, we found interesting similarities to previous microbiome results from non-carnivorous deep sea sponges, suggesting that the carnivorous sponges share characteristics of a previously hypothesized putative deep-sea sponge microbial community. Chemolithoautotrophic symbiosis was confirmed for C. methanophila through a microbial community with a high abundance of Methylococcales and very light isotopic δ13C and δ15N ratios (-60 to -66‰/3.5 to 5.2‰) compared to the other cladorhizid species (-22 to -24‰/8.5-10.5‰). We provide evidence for the presence of putative sulfur-oxidizing Gammaproteobacteria in the arctic cladorhizids; however, δ13C and δ15N signatures did not provide evidence for significant chemoautotrophic symbiosis in this case, and the slightly higher abundance of cladorhizids at the JMVF site compared to the nearby deep sea likely stem from an increased abundance of prey rather than a more direct vent association. The phylogenetic position of C. methanophila in relation to other carnivorous sponges was established using a three-gene phylogenetic analysis, and it was found to be closely related to other non-methanotrophic Cladorhiza species with a similar morphology included in the dataset, ... Article in Journal/Newspaper Arctic Jan Mayen Directory of Open Access Journals: DOAJ Articles Arctic Jan Mayen Frontiers in Microbiology 7 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Barbados Porifera methane seep hydrothermal vent isotope Cladorhizidae Microbiology QR1-502 |
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Barbados Porifera methane seep hydrothermal vent isotope Cladorhizidae Microbiology QR1-502 Jon Thomassen Hestetun Håkon Dahle Steffen Leth Jørgensen Bernt Rydland Olsen Hans Tore Rapp The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
topic_facet |
Barbados Porifera methane seep hydrothermal vent isotope Cladorhizidae Microbiology QR1-502 |
description |
As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of the methanotrophic carnivorous sponge Cladorhiza methanophila from a mud volcano-rich area at the Barbados Accretionary Prism (BAP), and five carnivorous species from the Jan Mayen Vent Field (JMVF) at the Arctic Mid-Ocean Ridge. Results from 16S rRNA microbiome data indicate the presence of a diverse assemblage of associated microorganisms in carnivorous sponges mainly from the Gamma- and Alphaproteobacteria, Flavobacteriaceae and Thaumarchaeota. While the abundance of particular groups varied throughout the dataset, we found interesting similarities to previous microbiome results from non-carnivorous deep sea sponges, suggesting that the carnivorous sponges share characteristics of a previously hypothesized putative deep-sea sponge microbial community. Chemolithoautotrophic symbiosis was confirmed for C. methanophila through a microbial community with a high abundance of Methylococcales and very light isotopic δ13C and δ15N ratios (-60 to -66‰/3.5 to 5.2‰) compared to the other cladorhizid species (-22 to -24‰/8.5-10.5‰). We provide evidence for the presence of putative sulfur-oxidizing Gammaproteobacteria in the arctic cladorhizids; however, δ13C and δ15N signatures did not provide evidence for significant chemoautotrophic symbiosis in this case, and the slightly higher abundance of cladorhizids at the JMVF site compared to the nearby deep sea likely stem from an increased abundance of prey rather than a more direct vent association. The phylogenetic position of C. methanophila in relation to other carnivorous sponges was established using a three-gene phylogenetic analysis, and it was found to be closely related to other non-methanotrophic Cladorhiza species with a similar morphology included in the dataset, ... |
format |
Article in Journal/Newspaper |
author |
Jon Thomassen Hestetun Håkon Dahle Steffen Leth Jørgensen Bernt Rydland Olsen Hans Tore Rapp |
author_facet |
Jon Thomassen Hestetun Håkon Dahle Steffen Leth Jørgensen Bernt Rydland Olsen Hans Tore Rapp |
author_sort |
Jon Thomassen Hestetun |
title |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_short |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_full |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_fullStr |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_full_unstemmed |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_sort |
microbiome and occurrence of methanotrophy in carnivorous sponges |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
https://doi.org/10.3389/fmicb.2016.01781 https://doaj.org/article/be4978033a1245e98710585ff9926440 |
geographic |
Arctic Jan Mayen |
geographic_facet |
Arctic Jan Mayen |
genre |
Arctic Jan Mayen |
genre_facet |
Arctic Jan Mayen |
op_source |
Frontiers in Microbiology, Vol 7 (2016) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01781/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.01781 https://doaj.org/article/be4978033a1245e98710585ff9926440 |
op_doi |
https://doi.org/10.3389/fmicb.2016.01781 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
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1766333848572919808 |