UltramicroELISA para la deteccion de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots
En este trabajo se establecen las condiciones optimas para la detección de anticuerpos IgM al glicolipido fenólico-I (GF-I) en muestras de sangre en papel de filtro utilizando el UltramicroELISA HANSEN y la tecnologia SUMA. Se estudiaron 300 donantes de sangre y 58 pacientes leprosos. Para estas dos...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
1994
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0036-46651994000200007 https://doaj.org/article/be1bffbe985f43748409817227d932ab |
id |
ftdoajarticles:oai:doaj.org/article:be1bffbe985f43748409817227d932ab |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:be1bffbe985f43748409817227d932ab 2024-09-09T19:28:23+00:00 UltramicroELISA para la deteccion de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots Adriana Torrella Rosa L. Solis Nerys Rodriguez Yadira Medina Martha Pita Ivis Perez Nubia Licourt 1994-04-01T00:00:00Z https://doi.org/10.1590/S0036-46651994000200007 https://doaj.org/article/be1bffbe985f43748409817227d932ab EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651994000200007 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651994000200007 0036-4665 1678-9946 https://doaj.org/article/be1bffbe985f43748409817227d932ab Revista do Instituto de Medicina Tropical de São Paulo, Vol 36, Iss 2, Pp 131-138 (1994) Lepra IgM anti-glicolipido fenolico-I (IgM ANTI GF-I) UltramicroELISA Sangre seca en papel de filtro Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1994 ftdoajarticles https://doi.org/10.1590/S0036-46651994000200007 2024-08-05T17:49:31Z En este trabajo se establecen las condiciones optimas para la detección de anticuerpos IgM al glicolipido fenólico-I (GF-I) en muestras de sangre en papel de filtro utilizando el UltramicroELISA HANSEN y la tecnologia SUMA. Se estudiaron 300 donantes de sangre y 58 pacientes leprosos. Para estas dos poblaciones se compararon los resultados de muestras de sangre seca colectadas en papel de filtro SS-2992 con los de suero, y se obtuvo una correlación de 0.919 para donantes de sangre, 0.969 para pacientes y 0.954 para el total de las dos poblaciones. Se obtuvo una coincidencia de 98% en pacientes y 96% en donantes. En la población de pacientes la sensibilidad fue de 93% y la especificidad de 100% para las muestras de sangre seca evaluadas por el UMELISA HANSEN, con respecto a las muestras de suero analizadas por esta misma tecnica. In this work the adequate conditions for the detection of IgM antibodies to PGL-I in eluates from dried blood spots using the UMELISA HANSEN and the SUMA technology are established. A total of 300 blood donors and 58 leprosy patients samples were studied. For both populations, in the previously established conditions, we compared the results of the eluates from dried blood spots with the serum samples, and was obtained a correlation of 0.919 in blood donors, 0.969 in patients, and 0.954 for the total of both populations. It was also obtained a level of agreemnet of 98% in patients and 96% in blood donors. In the patients population was found a sensitivity of 93% and an specificity of 100% for the eluates of dried blood spots evaluated by the UMELISA HANSEN in the comparison with the serum samples analized by the same assay. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 36 2 131 138 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Lepra IgM anti-glicolipido fenolico-I (IgM ANTI GF-I) UltramicroELISA Sangre seca en papel de filtro Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Lepra IgM anti-glicolipido fenolico-I (IgM ANTI GF-I) UltramicroELISA Sangre seca en papel de filtro Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Adriana Torrella Rosa L. Solis Nerys Rodriguez Yadira Medina Martha Pita Ivis Perez Nubia Licourt UltramicroELISA para la deteccion de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots |
topic_facet |
Lepra IgM anti-glicolipido fenolico-I (IgM ANTI GF-I) UltramicroELISA Sangre seca en papel de filtro Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
En este trabajo se establecen las condiciones optimas para la detección de anticuerpos IgM al glicolipido fenólico-I (GF-I) en muestras de sangre en papel de filtro utilizando el UltramicroELISA HANSEN y la tecnologia SUMA. Se estudiaron 300 donantes de sangre y 58 pacientes leprosos. Para estas dos poblaciones se compararon los resultados de muestras de sangre seca colectadas en papel de filtro SS-2992 con los de suero, y se obtuvo una correlación de 0.919 para donantes de sangre, 0.969 para pacientes y 0.954 para el total de las dos poblaciones. Se obtuvo una coincidencia de 98% en pacientes y 96% en donantes. En la población de pacientes la sensibilidad fue de 93% y la especificidad de 100% para las muestras de sangre seca evaluadas por el UMELISA HANSEN, con respecto a las muestras de suero analizadas por esta misma tecnica. In this work the adequate conditions for the detection of IgM antibodies to PGL-I in eluates from dried blood spots using the UMELISA HANSEN and the SUMA technology are established. A total of 300 blood donors and 58 leprosy patients samples were studied. For both populations, in the previously established conditions, we compared the results of the eluates from dried blood spots with the serum samples, and was obtained a correlation of 0.919 in blood donors, 0.969 in patients, and 0.954 for the total of both populations. It was also obtained a level of agreemnet of 98% in patients and 96% in blood donors. In the patients population was found a sensitivity of 93% and an specificity of 100% for the eluates of dried blood spots evaluated by the UMELISA HANSEN in the comparison with the serum samples analized by the same assay. |
format |
Article in Journal/Newspaper |
author |
Adriana Torrella Rosa L. Solis Nerys Rodriguez Yadira Medina Martha Pita Ivis Perez Nubia Licourt |
author_facet |
Adriana Torrella Rosa L. Solis Nerys Rodriguez Yadira Medina Martha Pita Ivis Perez Nubia Licourt |
author_sort |
Adriana Torrella |
title |
UltramicroELISA para la deteccion de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots |
title_short |
UltramicroELISA para la deteccion de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots |
title_full |
UltramicroELISA para la deteccion de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots |
title_fullStr |
UltramicroELISA para la deteccion de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots |
title_full_unstemmed |
UltramicroELISA para la deteccion de anticuerpos IgM al Mycobacterium leprae utilizando muestras de sangre seca UltramicroELISA assay for the detection of IgM antibodies to Mycobacterium leprae using eluates of dried blood spots |
title_sort |
ultramicroelisa para la deteccion de anticuerpos igm al mycobacterium leprae utilizando muestras de sangre seca ultramicroelisa assay for the detection of igm antibodies to mycobacterium leprae using eluates of dried blood spots |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1994 |
url |
https://doi.org/10.1590/S0036-46651994000200007 https://doaj.org/article/be1bffbe985f43748409817227d932ab |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 36, Iss 2, Pp 131-138 (1994) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651994000200007 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651994000200007 0036-4665 1678-9946 https://doaj.org/article/be1bffbe985f43748409817227d932ab |
op_doi |
https://doi.org/10.1590/S0036-46651994000200007 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
36 |
container_issue |
2 |
container_start_page |
131 |
op_container_end_page |
138 |
_version_ |
1809897620890451968 |