Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carri...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:bdf702c65c2c4c3d8c6779191e9a3c86 2023-05-15T15:11:26+02:00 Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice M. Marinho H. Langoni S. L. Oliveira V. M. F. Lima J. R. Peiró S. H. V. Perri R. Carreira 2005-06-01T00:00:00Z https://doi.org/10.1590/S1678-91992005000200009 https://doaj.org/article/bdf702c65c2c4c3d8c6779191e9a3c86 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000200009 1678-9199 https://doaj.org/article/bdf702c65c2c4c3d8c6779191e9a3c86 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 2, Pp 198-212 (2005) leptospirosis cytokines cellular immunity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2005 ftdoajarticles https://doi.org/10.1590/S1678-91992005000200009 2022-12-31T14:53:13Z Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the splenic cells of mice genetically selected as High (H) and Low (L) antibody producers, and inbred Balb/c mice infected with Leptospira interrogans serovar icterohaemorrhagiae. The IL-6 production varied from release peaks to inhibition in H, L, and Balb/c mice. Similar behavior was observed for IL-4, produced only by H and Balb/c mice. The three strains presented constant and elevated production of TNF-alpha until day 14, suggesting its effective participation in the initial phase of the infection. Meanwhile, all the three strains presented a constant and irregular IFN-gamma production, with release peaks between the 7th and 14th days in L mice. The H and Balb/c mice strains presented a higher tendency to Th2 response pattern, whereas L mice tended towards Th1 response. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 11 2 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
leptospirosis cytokines cellular immunity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
leptospirosis cytokines cellular immunity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 M. Marinho H. Langoni S. L. Oliveira V. M. F. Lima J. R. Peiró S. H. V. Perri R. Carreira Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice |
topic_facet |
leptospirosis cytokines cellular immunity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the splenic cells of mice genetically selected as High (H) and Low (L) antibody producers, and inbred Balb/c mice infected with Leptospira interrogans serovar icterohaemorrhagiae. The IL-6 production varied from release peaks to inhibition in H, L, and Balb/c mice. Similar behavior was observed for IL-4, produced only by H and Balb/c mice. The three strains presented constant and elevated production of TNF-alpha until day 14, suggesting its effective participation in the initial phase of the infection. Meanwhile, all the three strains presented a constant and irregular IFN-gamma production, with release peaks between the 7th and 14th days in L mice. The H and Balb/c mice strains presented a higher tendency to Th2 response pattern, whereas L mice tended towards Th1 response. |
format |
Article in Journal/Newspaper |
author |
M. Marinho H. Langoni S. L. Oliveira V. M. F. Lima J. R. Peiró S. H. V. Perri R. Carreira |
author_facet |
M. Marinho H. Langoni S. L. Oliveira V. M. F. Lima J. R. Peiró S. H. V. Perri R. Carreira |
author_sort |
M. Marinho |
title |
Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice |
title_short |
Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice |
title_full |
Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice |
title_fullStr |
Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice |
title_full_unstemmed |
Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice |
title_sort |
role of cytokines, no, and h2o2 on the immunopathology of leptospirosis in genetically selected mice |
publisher |
SciELO |
publishDate |
2005 |
url |
https://doi.org/10.1590/S1678-91992005000200009 https://doaj.org/article/bdf702c65c2c4c3d8c6779191e9a3c86 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 2, Pp 198-212 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000200009 1678-9199 https://doaj.org/article/bdf702c65c2c4c3d8c6779191e9a3c86 |
op_doi |
https://doi.org/10.1590/S1678-91992005000200009 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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11 |
container_issue |
2 |
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1766342288738353152 |