Comparison of total immunoglobulin G antibody responses to different protein fragments of Plasmodium vivax Reticulocyte binding protein 2b
Abstract Background Plasmodium vivax is emerging as the dominant and prevalent species causing malaria in near-elimination settings outside of Africa. Hypnozoites, the dormant liver stage parasite of P. vivax, are undetectable to any currently available diagnostic test, yet are a major reservoir for...
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ftdoajarticles:oai:doaj.org/article:bdeaa3d1dceb4c67805ac42c8632e113 2023-05-15T15:14:55+02:00 Comparison of total immunoglobulin G antibody responses to different protein fragments of Plasmodium vivax Reticulocyte binding protein 2b Caitlin Bourke Eizo Takashima Li-Jin Chan Melanie H. Dietrich Ramin Mazhari Michael White Jetsumon Sattabongkot Wai-Hong Tham Takafumi Tsuboi Ivo Mueller Rhea Longley 2022-03-01T00:00:00Z https://doi.org/10.1186/s12936-022-04085-x https://doaj.org/article/bdeaa3d1dceb4c67805ac42c8632e113 EN eng BMC https://doi.org/10.1186/s12936-022-04085-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04085-x 1475-2875 https://doaj.org/article/bdeaa3d1dceb4c67805ac42c8632e113 Malaria Journal, Vol 21, Iss 1, Pp 1-10 (2022) Plasmodium vivax Reticulocyte binding protein 2b IgG Serological markers Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04085-x 2022-12-31T16:13:20Z Abstract Background Plasmodium vivax is emerging as the dominant and prevalent species causing malaria in near-elimination settings outside of Africa. Hypnozoites, the dormant liver stage parasite of P. vivax, are undetectable to any currently available diagnostic test, yet are a major reservoir for transmission. Advances have been made to harness the naturally acquired immune response to identify recent exposure to P. vivax blood-stage parasites and, therefore, infer the presence of hypnozoites. This in-development diagnostic is currently able to detect infections within the last 9-months with 80% sensitivity and 80% specificity. Further work is required to optimize protein expression and protein constructs used for antibody detection. Methods The antibody response against the top performing predictor of recent infection, P. vivax reticulocyte binding protein 2b (PvRBP2b), was tested against multiple fragments of different sizes and from different expression systems. The IgG induced against the recombinant PvRBP2b fragments in P. vivax infected individuals was measured at the time of infection and in a year-long observational cohort; both conducted in Thailand. Results The antibody responses to some but not all different sized fragments of PvRBP2b protein are highly correlated with each other, significantly higher 1-week post-P. vivax infection, and show potential for use as predictors of recent P. vivax infection. Conclusions To achieve P. vivax elimination goals, novel diagnostics are required to aid in detection of hidden parasite reservoirs. PvRBP2b was previously shown to be the top candidate for single-antigen classification of recent P. vivax exposure and here, it is concluded that several alternative recombinant PvRBP2b fragments can achieve equal sensitivity and specificity at predicting recent P. vivax exposure. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 21 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Plasmodium vivax Reticulocyte binding protein 2b IgG Serological markers Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium vivax Reticulocyte binding protein 2b IgG Serological markers Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Caitlin Bourke Eizo Takashima Li-Jin Chan Melanie H. Dietrich Ramin Mazhari Michael White Jetsumon Sattabongkot Wai-Hong Tham Takafumi Tsuboi Ivo Mueller Rhea Longley Comparison of total immunoglobulin G antibody responses to different protein fragments of Plasmodium vivax Reticulocyte binding protein 2b |
topic_facet |
Plasmodium vivax Reticulocyte binding protein 2b IgG Serological markers Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium vivax is emerging as the dominant and prevalent species causing malaria in near-elimination settings outside of Africa. Hypnozoites, the dormant liver stage parasite of P. vivax, are undetectable to any currently available diagnostic test, yet are a major reservoir for transmission. Advances have been made to harness the naturally acquired immune response to identify recent exposure to P. vivax blood-stage parasites and, therefore, infer the presence of hypnozoites. This in-development diagnostic is currently able to detect infections within the last 9-months with 80% sensitivity and 80% specificity. Further work is required to optimize protein expression and protein constructs used for antibody detection. Methods The antibody response against the top performing predictor of recent infection, P. vivax reticulocyte binding protein 2b (PvRBP2b), was tested against multiple fragments of different sizes and from different expression systems. The IgG induced against the recombinant PvRBP2b fragments in P. vivax infected individuals was measured at the time of infection and in a year-long observational cohort; both conducted in Thailand. Results The antibody responses to some but not all different sized fragments of PvRBP2b protein are highly correlated with each other, significantly higher 1-week post-P. vivax infection, and show potential for use as predictors of recent P. vivax infection. Conclusions To achieve P. vivax elimination goals, novel diagnostics are required to aid in detection of hidden parasite reservoirs. PvRBP2b was previously shown to be the top candidate for single-antigen classification of recent P. vivax exposure and here, it is concluded that several alternative recombinant PvRBP2b fragments can achieve equal sensitivity and specificity at predicting recent P. vivax exposure. |
format |
Article in Journal/Newspaper |
author |
Caitlin Bourke Eizo Takashima Li-Jin Chan Melanie H. Dietrich Ramin Mazhari Michael White Jetsumon Sattabongkot Wai-Hong Tham Takafumi Tsuboi Ivo Mueller Rhea Longley |
author_facet |
Caitlin Bourke Eizo Takashima Li-Jin Chan Melanie H. Dietrich Ramin Mazhari Michael White Jetsumon Sattabongkot Wai-Hong Tham Takafumi Tsuboi Ivo Mueller Rhea Longley |
author_sort |
Caitlin Bourke |
title |
Comparison of total immunoglobulin G antibody responses to different protein fragments of Plasmodium vivax Reticulocyte binding protein 2b |
title_short |
Comparison of total immunoglobulin G antibody responses to different protein fragments of Plasmodium vivax Reticulocyte binding protein 2b |
title_full |
Comparison of total immunoglobulin G antibody responses to different protein fragments of Plasmodium vivax Reticulocyte binding protein 2b |
title_fullStr |
Comparison of total immunoglobulin G antibody responses to different protein fragments of Plasmodium vivax Reticulocyte binding protein 2b |
title_full_unstemmed |
Comparison of total immunoglobulin G antibody responses to different protein fragments of Plasmodium vivax Reticulocyte binding protein 2b |
title_sort |
comparison of total immunoglobulin g antibody responses to different protein fragments of plasmodium vivax reticulocyte binding protein 2b |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12936-022-04085-x https://doaj.org/article/bdeaa3d1dceb4c67805ac42c8632e113 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 21, Iss 1, Pp 1-10 (2022) |
op_relation |
https://doi.org/10.1186/s12936-022-04085-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04085-x 1475-2875 https://doaj.org/article/bdeaa3d1dceb4c67805ac42c8632e113 |
op_doi |
https://doi.org/10.1186/s12936-022-04085-x |
container_title |
Malaria Journal |
container_volume |
21 |
container_issue |
1 |
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1766345318690979840 |