Secondary bacterial infections and antibiotic resistance among tungiasis patients in Western, Kenya.
Tungiasis or jigger infestation is a parasitic disease caused by the female sand flea Tunga penetrans. Secondary infection of the lesions caused by this flea is common in endemic communities. This study sought to shed light on the bacterial pathogens causing secondary infections in tungiasis lesions...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0005901 https://doaj.org/article/bddb06109aea414cafca4f9b11758ba6 |
id |
ftdoajarticles:oai:doaj.org/article:bddb06109aea414cafca4f9b11758ba6 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:bddb06109aea414cafca4f9b11758ba6 2023-05-15T15:11:55+02:00 Secondary bacterial infections and antibiotic resistance among tungiasis patients in Western, Kenya. Ruth Monyenye Nyangacha David Odongo Florence Oyieke Missiani Ochwoto Richard Korir Ronald Kiprotich Ngetich Gladys Nginya Olipher Makwaga Christine Bii Peter Mwitari Festus Tolo 2017-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005901 https://doaj.org/article/bddb06109aea414cafca4f9b11758ba6 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5607213?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005901 https://doaj.org/article/bddb06109aea414cafca4f9b11758ba6 PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005901 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005901 2022-12-31T10:45:51Z Tungiasis or jigger infestation is a parasitic disease caused by the female sand flea Tunga penetrans. Secondary infection of the lesions caused by this flea is common in endemic communities. This study sought to shed light on the bacterial pathogens causing secondary infections in tungiasis lesions and their susceptibility profiles to commonly prescribed antibiotics. Participants were recruited with the help of Community Health Workers. Swabs were taken from lesions which showed signs of secondary infection. Identification of suspected bacteria colonies was done by colony morphology, Gram staining, and biochemical tests. The Kirby Bauer disc diffusion test was used to determine the drug susceptibility profiles. Out of 37 participants, from whom swabs were collected, specimen were positive in 29 and 8 had no growth. From these, 10 different strains of bacteria were isolated. Two were Gram positive bacteria and they were, Staphylococcus epidermidis (38.3%) and Staphylococcus aureus (21.3%). Eight were Gram negative namely Enterobacter cloacae (8.5%), Proteus species (8.5%), Klebsiellla species (6.4%), Aeromonas sobria (4.3%), Citrobacter species (4.3%), Proteus mirabillis(4.3%), Enterobacter amnigenus (2.1%) and Klebsiella pneumoniae (2.1%). The methicillin resistant S. aureus (MRSA) isolated were also resistant to clindamycin, kanamycin, erythromycin, nalidixic acid, trimethorprim sulfamethoxazole and tetracycline. All the Gram negative and Gram positive bacteria isolates were sensitive to gentamicin and norfloxacin drugs. Results from this study confirms the presence of resistant bacteria in tungiasis lesions hence highlighting the significance of secondary infection of the lesions in endemic communties. This therefore suggests that antimicrobial susceptibility testing may be considered to guide in identification of appropriate antibiotics and treatment therapy among tungiasis patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tunga ENVELOPE(8.683,8.683,62.698,62.698) PLOS Neglected Tropical Diseases 11 9 e0005901 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ruth Monyenye Nyangacha David Odongo Florence Oyieke Missiani Ochwoto Richard Korir Ronald Kiprotich Ngetich Gladys Nginya Olipher Makwaga Christine Bii Peter Mwitari Festus Tolo Secondary bacterial infections and antibiotic resistance among tungiasis patients in Western, Kenya. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Tungiasis or jigger infestation is a parasitic disease caused by the female sand flea Tunga penetrans. Secondary infection of the lesions caused by this flea is common in endemic communities. This study sought to shed light on the bacterial pathogens causing secondary infections in tungiasis lesions and their susceptibility profiles to commonly prescribed antibiotics. Participants were recruited with the help of Community Health Workers. Swabs were taken from lesions which showed signs of secondary infection. Identification of suspected bacteria colonies was done by colony morphology, Gram staining, and biochemical tests. The Kirby Bauer disc diffusion test was used to determine the drug susceptibility profiles. Out of 37 participants, from whom swabs were collected, specimen were positive in 29 and 8 had no growth. From these, 10 different strains of bacteria were isolated. Two were Gram positive bacteria and they were, Staphylococcus epidermidis (38.3%) and Staphylococcus aureus (21.3%). Eight were Gram negative namely Enterobacter cloacae (8.5%), Proteus species (8.5%), Klebsiellla species (6.4%), Aeromonas sobria (4.3%), Citrobacter species (4.3%), Proteus mirabillis(4.3%), Enterobacter amnigenus (2.1%) and Klebsiella pneumoniae (2.1%). The methicillin resistant S. aureus (MRSA) isolated were also resistant to clindamycin, kanamycin, erythromycin, nalidixic acid, trimethorprim sulfamethoxazole and tetracycline. All the Gram negative and Gram positive bacteria isolates were sensitive to gentamicin and norfloxacin drugs. Results from this study confirms the presence of resistant bacteria in tungiasis lesions hence highlighting the significance of secondary infection of the lesions in endemic communties. This therefore suggests that antimicrobial susceptibility testing may be considered to guide in identification of appropriate antibiotics and treatment therapy among tungiasis patients. |
format |
Article in Journal/Newspaper |
author |
Ruth Monyenye Nyangacha David Odongo Florence Oyieke Missiani Ochwoto Richard Korir Ronald Kiprotich Ngetich Gladys Nginya Olipher Makwaga Christine Bii Peter Mwitari Festus Tolo |
author_facet |
Ruth Monyenye Nyangacha David Odongo Florence Oyieke Missiani Ochwoto Richard Korir Ronald Kiprotich Ngetich Gladys Nginya Olipher Makwaga Christine Bii Peter Mwitari Festus Tolo |
author_sort |
Ruth Monyenye Nyangacha |
title |
Secondary bacterial infections and antibiotic resistance among tungiasis patients in Western, Kenya. |
title_short |
Secondary bacterial infections and antibiotic resistance among tungiasis patients in Western, Kenya. |
title_full |
Secondary bacterial infections and antibiotic resistance among tungiasis patients in Western, Kenya. |
title_fullStr |
Secondary bacterial infections and antibiotic resistance among tungiasis patients in Western, Kenya. |
title_full_unstemmed |
Secondary bacterial infections and antibiotic resistance among tungiasis patients in Western, Kenya. |
title_sort |
secondary bacterial infections and antibiotic resistance among tungiasis patients in western, kenya. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005901 https://doaj.org/article/bddb06109aea414cafca4f9b11758ba6 |
long_lat |
ENVELOPE(8.683,8.683,62.698,62.698) |
geographic |
Arctic Tunga |
geographic_facet |
Arctic Tunga |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005901 (2017) |
op_relation |
http://europepmc.org/articles/PMC5607213?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005901 https://doaj.org/article/bddb06109aea414cafca4f9b11758ba6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005901 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
9 |
container_start_page |
e0005901 |
_version_ |
1766342696338718720 |