The Richness of Sarcocystis Species in the Common Gull ( Larus canus ) and Black-Headed Gull ( Larus ridibundus ) from Lithuania
The common gull ( Larus canus ) and the black-headed gull ( Larus ridibundus ) are common waterbird species in Lithuania. Until now, the composition of Sarcocystis species in these birds was unknown. The current study aimed to identify Sarcocystis spp. by the morphological examination of sarcocysts...
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2023
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ftdoajarticles:oai:doaj.org/article:bdd956463fbf4ed6b03ab56a760be7a0 2023-07-23T04:20:12+02:00 The Richness of Sarcocystis Species in the Common Gull ( Larus canus ) and Black-Headed Gull ( Larus ridibundus ) from Lithuania Evelina Juozaitytė-Ngugu Petras Prakas 2023-05-01T00:00:00Z https://doi.org/10.3390/parasitologia3020018 https://doaj.org/article/bdd956463fbf4ed6b03ab56a760be7a0 EN eng MDPI AG https://www.mdpi.com/2673-6772/3/2/18 https://doaj.org/toc/2673-6772 doi:10.3390/parasitologia3020018 2673-6772 https://doaj.org/article/bdd956463fbf4ed6b03ab56a760be7a0 Parasitologia, Vol 3, Iss 18, Pp 172-180 (2023) Sarcocystis gulls infection rates ITS1 genetic variation Infectious and parasitic diseases RC109-216 Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3390/parasitologia3020018 2023-07-02T00:37:26Z The common gull ( Larus canus ) and the black-headed gull ( Larus ridibundus ) are common waterbird species in Lithuania. Until now, the composition of Sarcocystis species in these birds was unknown. The current study aimed to identify Sarcocystis spp. by the morphological examination of sarcocysts found in the muscle tissues and by DNA sequence analysis. Between 2011 and 2019, the leg muscles of 42 common gulls and 63 black-headed gulls were tested for Sarcocystis spp. Based on the methylene blue staining of squashed muscle samples, sarcocysts were detected in six common gulls (14.3%) and in six black-headed gulls (9.5%). Under a light microscope, one type of microcyst was observed. Sarcocysts were thread-like (2860–8250 × 40–180 μm) and had a smooth and thin (0.8–1.4 μm) cyst wall, while bradyzoites were banana-shaped and 5.0–9.2 × 1.3–2.4 μm in size. The sequencing of complete ITS1 showed the presence of S . columbae , S . halieti and S . wobeseri in the common gull and S . columbae and S . halieti in the black-headed gull. The highest intraspecific genetic variability was established for S . halieti , which is characterized by a wide host range. This species is considered to be pathogenic, therefore further histopathological examination of the various organs of gulls is needed. Article in Journal/Newspaper Larus canus Black-headed Gull Larus ridibundus Directory of Open Access Journals: DOAJ Articles Parasitologia 3 2 172 180 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Sarcocystis gulls infection rates ITS1 genetic variation Infectious and parasitic diseases RC109-216 Biology (General) QH301-705.5 |
spellingShingle |
Sarcocystis gulls infection rates ITS1 genetic variation Infectious and parasitic diseases RC109-216 Biology (General) QH301-705.5 Evelina Juozaitytė-Ngugu Petras Prakas The Richness of Sarcocystis Species in the Common Gull ( Larus canus ) and Black-Headed Gull ( Larus ridibundus ) from Lithuania |
topic_facet |
Sarcocystis gulls infection rates ITS1 genetic variation Infectious and parasitic diseases RC109-216 Biology (General) QH301-705.5 |
description |
The common gull ( Larus canus ) and the black-headed gull ( Larus ridibundus ) are common waterbird species in Lithuania. Until now, the composition of Sarcocystis species in these birds was unknown. The current study aimed to identify Sarcocystis spp. by the morphological examination of sarcocysts found in the muscle tissues and by DNA sequence analysis. Between 2011 and 2019, the leg muscles of 42 common gulls and 63 black-headed gulls were tested for Sarcocystis spp. Based on the methylene blue staining of squashed muscle samples, sarcocysts were detected in six common gulls (14.3%) and in six black-headed gulls (9.5%). Under a light microscope, one type of microcyst was observed. Sarcocysts were thread-like (2860–8250 × 40–180 μm) and had a smooth and thin (0.8–1.4 μm) cyst wall, while bradyzoites were banana-shaped and 5.0–9.2 × 1.3–2.4 μm in size. The sequencing of complete ITS1 showed the presence of S . columbae , S . halieti and S . wobeseri in the common gull and S . columbae and S . halieti in the black-headed gull. The highest intraspecific genetic variability was established for S . halieti , which is characterized by a wide host range. This species is considered to be pathogenic, therefore further histopathological examination of the various organs of gulls is needed. |
format |
Article in Journal/Newspaper |
author |
Evelina Juozaitytė-Ngugu Petras Prakas |
author_facet |
Evelina Juozaitytė-Ngugu Petras Prakas |
author_sort |
Evelina Juozaitytė-Ngugu |
title |
The Richness of Sarcocystis Species in the Common Gull ( Larus canus ) and Black-Headed Gull ( Larus ridibundus ) from Lithuania |
title_short |
The Richness of Sarcocystis Species in the Common Gull ( Larus canus ) and Black-Headed Gull ( Larus ridibundus ) from Lithuania |
title_full |
The Richness of Sarcocystis Species in the Common Gull ( Larus canus ) and Black-Headed Gull ( Larus ridibundus ) from Lithuania |
title_fullStr |
The Richness of Sarcocystis Species in the Common Gull ( Larus canus ) and Black-Headed Gull ( Larus ridibundus ) from Lithuania |
title_full_unstemmed |
The Richness of Sarcocystis Species in the Common Gull ( Larus canus ) and Black-Headed Gull ( Larus ridibundus ) from Lithuania |
title_sort |
richness of sarcocystis species in the common gull ( larus canus ) and black-headed gull ( larus ridibundus ) from lithuania |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/parasitologia3020018 https://doaj.org/article/bdd956463fbf4ed6b03ab56a760be7a0 |
genre |
Larus canus Black-headed Gull Larus ridibundus |
genre_facet |
Larus canus Black-headed Gull Larus ridibundus |
op_source |
Parasitologia, Vol 3, Iss 18, Pp 172-180 (2023) |
op_relation |
https://www.mdpi.com/2673-6772/3/2/18 https://doaj.org/toc/2673-6772 doi:10.3390/parasitologia3020018 2673-6772 https://doaj.org/article/bdd956463fbf4ed6b03ab56a760be7a0 |
op_doi |
https://doi.org/10.3390/parasitologia3020018 |
container_title |
Parasitologia |
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3 |
container_issue |
2 |
container_start_page |
172 |
op_container_end_page |
180 |
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1772184028377513984 |