How new molecular tools can help bugbusters: a Burkholderia cepacia complex outbreak investigation
ABSTRACT An outbreak of bloodstream infection (BSI) caused by members of the Burkholderia cepacia complex (Bcc) took place from March 2012 until April 2014 involving thirteen patients. Aim. To describe an outbreak investigation of BSI Bcc and showing how genetic sequencing tools contributed to confi...
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ftdoajarticles:oai:doaj.org/article:bdaa538bb8ed4c0b8a9b6436d330e27c 2024-09-09T19:26:13+00:00 How new molecular tools can help bugbusters: a Burkholderia cepacia complex outbreak investigation Amanda Luiz Pires Maciel Cristiane Schmitt Marcia Maria Baraldi Caio de Lima Silva Luiz Felipe Valter de Oliveira Jorge Luiz Mello Sampaio Rogerio Ferreira Lourenço Anna Sara Levin Icaro Boszczowski 2020-08-01T00:00:00Z https://doi.org/10.1590/s1678-9946202062059 https://doaj.org/article/bdaa538bb8ed4c0b8a9b6436d330e27c EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100228&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062059 https://doaj.org/article/bdaa538bb8ed4c0b8a9b6436d330e27c Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) Burkholderia cepacia Outbreak investigation Bloodstream infection Next generation sequencing Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1590/s1678-9946202062059 2024-08-05T17:49:31Z ABSTRACT An outbreak of bloodstream infection (BSI) caused by members of the Burkholderia cepacia complex (Bcc) took place from March 2012 until April 2014 involving thirteen patients. Aim. To describe an outbreak investigation of BSI Bcc and showing how genetic sequencing tools contributed to confirm the hypothesis of extrinsic contamination proposed by an observational study. Methods. The Infection Control Department revised and reinforced good practices of infusion therapy and catheter care, visits to affected wards, a case control study, and environmental screening based on the case-control findings. Results. Data from the case-control study found an association of cases with central venous catheter (OR 1.36; CI 1.15-1.67) and intravenous cisatracurium use (OR 10.75; CI 1.67-68.89). Visits to the operatory block revealed problems related to the cold chain used for the preservation of thermolabile cisatracurium. We could not retrieve Bcc from environmental samples using classic microbiology. New samples from the same surfaces were obtained for genetic sequencing. Bcc was identified in the cooler box, refrigerator and reusable ice packages. Conclusion. Environmental screening using genetic sequencing proved to be a useful tool for confirming our hypothesis of extrinsic contamination raised by the case-control study. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 62 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Burkholderia cepacia Outbreak investigation Bloodstream infection Next generation sequencing Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Burkholderia cepacia Outbreak investigation Bloodstream infection Next generation sequencing Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Amanda Luiz Pires Maciel Cristiane Schmitt Marcia Maria Baraldi Caio de Lima Silva Luiz Felipe Valter de Oliveira Jorge Luiz Mello Sampaio Rogerio Ferreira Lourenço Anna Sara Levin Icaro Boszczowski How new molecular tools can help bugbusters: a Burkholderia cepacia complex outbreak investigation |
topic_facet |
Burkholderia cepacia Outbreak investigation Bloodstream infection Next generation sequencing Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT An outbreak of bloodstream infection (BSI) caused by members of the Burkholderia cepacia complex (Bcc) took place from March 2012 until April 2014 involving thirteen patients. Aim. To describe an outbreak investigation of BSI Bcc and showing how genetic sequencing tools contributed to confirm the hypothesis of extrinsic contamination proposed by an observational study. Methods. The Infection Control Department revised and reinforced good practices of infusion therapy and catheter care, visits to affected wards, a case control study, and environmental screening based on the case-control findings. Results. Data from the case-control study found an association of cases with central venous catheter (OR 1.36; CI 1.15-1.67) and intravenous cisatracurium use (OR 10.75; CI 1.67-68.89). Visits to the operatory block revealed problems related to the cold chain used for the preservation of thermolabile cisatracurium. We could not retrieve Bcc from environmental samples using classic microbiology. New samples from the same surfaces were obtained for genetic sequencing. Bcc was identified in the cooler box, refrigerator and reusable ice packages. Conclusion. Environmental screening using genetic sequencing proved to be a useful tool for confirming our hypothesis of extrinsic contamination raised by the case-control study. |
format |
Article in Journal/Newspaper |
author |
Amanda Luiz Pires Maciel Cristiane Schmitt Marcia Maria Baraldi Caio de Lima Silva Luiz Felipe Valter de Oliveira Jorge Luiz Mello Sampaio Rogerio Ferreira Lourenço Anna Sara Levin Icaro Boszczowski |
author_facet |
Amanda Luiz Pires Maciel Cristiane Schmitt Marcia Maria Baraldi Caio de Lima Silva Luiz Felipe Valter de Oliveira Jorge Luiz Mello Sampaio Rogerio Ferreira Lourenço Anna Sara Levin Icaro Boszczowski |
author_sort |
Amanda Luiz Pires Maciel |
title |
How new molecular tools can help bugbusters: a Burkholderia cepacia complex outbreak investigation |
title_short |
How new molecular tools can help bugbusters: a Burkholderia cepacia complex outbreak investigation |
title_full |
How new molecular tools can help bugbusters: a Burkholderia cepacia complex outbreak investigation |
title_fullStr |
How new molecular tools can help bugbusters: a Burkholderia cepacia complex outbreak investigation |
title_full_unstemmed |
How new molecular tools can help bugbusters: a Burkholderia cepacia complex outbreak investigation |
title_sort |
how new molecular tools can help bugbusters: a burkholderia cepacia complex outbreak investigation |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2020 |
url |
https://doi.org/10.1590/s1678-9946202062059 https://doaj.org/article/bdaa538bb8ed4c0b8a9b6436d330e27c |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100228&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062059 https://doaj.org/article/bdaa538bb8ed4c0b8a9b6436d330e27c |
op_doi |
https://doi.org/10.1590/s1678-9946202062059 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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62 |
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