Isolation of Methicillin Resistant Staphylococcus aureus (MRSA) from Rattus rattus from Adhamiyah district in Baghdad governorate
This study focused on the isolation of methicillin resistance Staphylococcus aureus (MRSA) from black rats (Rattus rattus) from Adhamiyah district. A Total of 30 black rats (Rattus rattus) used in this study. Specimens from the upper respiratory tract, feces, and urine were collected in aseptic cond...
Main Authors: | , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Mirror of Research in Veterinary Sciences and Animals (MRVSA)
2015
|
Subjects: | |
Online Access: | https://doaj.org/article/bd9354b733c64e3dbf211367e7cf30d8 |
id |
ftdoajarticles:oai:doaj.org/article:bd9354b733c64e3dbf211367e7cf30d8 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:bd9354b733c64e3dbf211367e7cf30d8 2023-05-15T18:04:53+02:00 Isolation of Methicillin Resistant Staphylococcus aureus (MRSA) from Rattus rattus from Adhamiyah district in Baghdad governorate Sahar K. Khalaf Nagham M. Ayyal Abdulkarim J. Karim2 Jenan M. Khalaf 2015-11-01T00:00:00Z https://doaj.org/article/bd9354b733c64e3dbf211367e7cf30d8 EN eng Mirror of Research in Veterinary Sciences and Animals (MRVSA) http://mrvsa.com/upload/article%202%20Isolation%20of%20Methicillin%20Resistant%20Staphylococcus%20aureus%20(MRSA)%20from%20Rattus%20rattus%20from%20Adhamiyah%20district%20in%20Baghdad%20governorate.pdf https://doaj.org/toc/2520-324X 2520-324X https://doaj.org/article/bd9354b733c64e3dbf211367e7cf30d8 Mirror of Research in Veterinary Sciences and Animals, Vol 4, Iss 3, Pp 9-22 (2015) MRSA pharyngeal urine feces rat Baghdad Animal culture SF1-1100 Veterinary medicine SF600-1100 article 2015 ftdoajarticles 2022-12-31T00:48:35Z This study focused on the isolation of methicillin resistance Staphylococcus aureus (MRSA) from black rats (Rattus rattus) from Adhamiyah district. A Total of 30 black rats (Rattus rattus) used in this study. Specimens from the upper respiratory tract, feces, and urine were collected in aseptic conditions for bacteriological culture. Standard diagnostic methods were used to isolate Gram- positive (+ve) bacteria. All samples were cultured on mannitol agar, a differential media for staphylococci (Staph), and incubated at 37°C for 24 hours. Gram stain was achieved to identify Staph bacteria. Also, the biochemical tests and the API Staph system were applied for identification of Staphylococcus aureus. The results of this study showed that twenty-eight rats out of thirty were harbored Staphylococci. In this study, four samples of upper pharyngeal swab were negative for Staphylococcus aureus, while the other 26 samples were positive. In urine samples, 11 samples were negative, and the rest (19 samples) were positive for Staphylococcus aureus. All examined rats showed no Staph growth in fecal samples. The isolated staphylococci distributed as coagulase-negative (S. xylosus, 2 (3.57); S. epidermidis, 6 (10.71); and S. sciuri, 3 (5.35), and coagulase positive (S. aureus, 45). Out of these forty-five isolates, 26 (45.42%) were from the deep pharyngeal swap and the rest 19 (33.92 %) were isolated from urine. The occurrence of MRSA was investigated by disc diffusion method. The results indicated that out of the 45 isolates, 42 (93.9%) and 3 (6.1%) were sensitive and resistance, respectively, to the Methicillin. All MRSA was obtained from urine. However, no Staphylococci were isolated from feces. In conclusion, this study approved that MRSA isolated from black rat (Rattus rattus) from Adhamiyah district could play a crucial role in spreading diseases to human and animal via dried urine, which is un-visible by the naked eyes. Consequently, the authors recommend another future studies to investigate in details the actual role ... Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
MRSA pharyngeal urine feces rat Baghdad Animal culture SF1-1100 Veterinary medicine SF600-1100 |
spellingShingle |
MRSA pharyngeal urine feces rat Baghdad Animal culture SF1-1100 Veterinary medicine SF600-1100 Sahar K. Khalaf Nagham M. Ayyal Abdulkarim J. Karim2 Jenan M. Khalaf Isolation of Methicillin Resistant Staphylococcus aureus (MRSA) from Rattus rattus from Adhamiyah district in Baghdad governorate |
topic_facet |
MRSA pharyngeal urine feces rat Baghdad Animal culture SF1-1100 Veterinary medicine SF600-1100 |
description |
This study focused on the isolation of methicillin resistance Staphylococcus aureus (MRSA) from black rats (Rattus rattus) from Adhamiyah district. A Total of 30 black rats (Rattus rattus) used in this study. Specimens from the upper respiratory tract, feces, and urine were collected in aseptic conditions for bacteriological culture. Standard diagnostic methods were used to isolate Gram- positive (+ve) bacteria. All samples were cultured on mannitol agar, a differential media for staphylococci (Staph), and incubated at 37°C for 24 hours. Gram stain was achieved to identify Staph bacteria. Also, the biochemical tests and the API Staph system were applied for identification of Staphylococcus aureus. The results of this study showed that twenty-eight rats out of thirty were harbored Staphylococci. In this study, four samples of upper pharyngeal swab were negative for Staphylococcus aureus, while the other 26 samples were positive. In urine samples, 11 samples were negative, and the rest (19 samples) were positive for Staphylococcus aureus. All examined rats showed no Staph growth in fecal samples. The isolated staphylococci distributed as coagulase-negative (S. xylosus, 2 (3.57); S. epidermidis, 6 (10.71); and S. sciuri, 3 (5.35), and coagulase positive (S. aureus, 45). Out of these forty-five isolates, 26 (45.42%) were from the deep pharyngeal swap and the rest 19 (33.92 %) were isolated from urine. The occurrence of MRSA was investigated by disc diffusion method. The results indicated that out of the 45 isolates, 42 (93.9%) and 3 (6.1%) were sensitive and resistance, respectively, to the Methicillin. All MRSA was obtained from urine. However, no Staphylococci were isolated from feces. In conclusion, this study approved that MRSA isolated from black rat (Rattus rattus) from Adhamiyah district could play a crucial role in spreading diseases to human and animal via dried urine, which is un-visible by the naked eyes. Consequently, the authors recommend another future studies to investigate in details the actual role ... |
format |
Article in Journal/Newspaper |
author |
Sahar K. Khalaf Nagham M. Ayyal Abdulkarim J. Karim2 Jenan M. Khalaf |
author_facet |
Sahar K. Khalaf Nagham M. Ayyal Abdulkarim J. Karim2 Jenan M. Khalaf |
author_sort |
Sahar K. Khalaf |
title |
Isolation of Methicillin Resistant Staphylococcus aureus (MRSA) from Rattus rattus from Adhamiyah district in Baghdad governorate |
title_short |
Isolation of Methicillin Resistant Staphylococcus aureus (MRSA) from Rattus rattus from Adhamiyah district in Baghdad governorate |
title_full |
Isolation of Methicillin Resistant Staphylococcus aureus (MRSA) from Rattus rattus from Adhamiyah district in Baghdad governorate |
title_fullStr |
Isolation of Methicillin Resistant Staphylococcus aureus (MRSA) from Rattus rattus from Adhamiyah district in Baghdad governorate |
title_full_unstemmed |
Isolation of Methicillin Resistant Staphylococcus aureus (MRSA) from Rattus rattus from Adhamiyah district in Baghdad governorate |
title_sort |
isolation of methicillin resistant staphylococcus aureus (mrsa) from rattus rattus from adhamiyah district in baghdad governorate |
publisher |
Mirror of Research in Veterinary Sciences and Animals (MRVSA) |
publishDate |
2015 |
url |
https://doaj.org/article/bd9354b733c64e3dbf211367e7cf30d8 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Mirror of Research in Veterinary Sciences and Animals, Vol 4, Iss 3, Pp 9-22 (2015) |
op_relation |
http://mrvsa.com/upload/article%202%20Isolation%20of%20Methicillin%20Resistant%20Staphylococcus%20aureus%20(MRSA)%20from%20Rattus%20rattus%20from%20Adhamiyah%20district%20in%20Baghdad%20governorate.pdf https://doaj.org/toc/2520-324X 2520-324X https://doaj.org/article/bd9354b733c64e3dbf211367e7cf30d8 |
_version_ |
1766176287047548928 |