Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat.
CD4+ T cells play critical roles in mediating adaptive immunity to a variety of pathogens. Recently, new subset of CD4+T named as T helper 9 cells that express the prototypical interleukin-9 (IL-9) cytokine have been recognized in human and mice models during different parasitic infections. Haemonch...
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ftdoajarticles:oai:doaj.org/article:bd814cd2fb3c4643affddb8565ef498b 2023-05-15T15:17:52+02:00 Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat. Muhammad Ali Memon Muhammad Ali-Ul-Husnain Naqvi Huang Xin Liang Meng Muhammad Waqqas Hasan Muhammad Haseeb Shakeel Ahmed Lakho Kalilixiati Aimulajiang Yongqian Bu Lixin Xu Xiaokai Song Xiangrui Li Ruofeng Yan 2020-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008218 https://doaj.org/article/bd814cd2fb3c4643affddb8565ef498b EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008218 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008218 https://doaj.org/article/bd814cd2fb3c4643affddb8565ef498b PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008218 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008218 2022-12-31T07:46:37Z CD4+ T cells play critical roles in mediating adaptive immunity to a variety of pathogens. Recently, new subset of CD4+T named as T helper 9 cells that express the prototypical interleukin-9 (IL-9) cytokine have been recognized in human and mice models during different parasitic infections. Haemonchus contortus is a gastrointestinal nematode of small ruminants which cause high mortality in young animals. During infection, Excretory and Secretary Products (ESPs) are released in the host body. No other study has reported yet on immunomodulatory dynamics of H. contortus ESPs on Th9 immune response in vitro or in vivo. In this study, immunomodulatory effects of ESPs (5, 10, 20, 40, 80; μg/mL) incubated with goat PBMCs on Th9 cells, IL-9 immune response and TGF-β/Smad signaling regulator were evaluated in vitro. Moreover, for in vivo study, goats were infected with different doses (P-800, P-2400, and P-8000) of H. contortus infective larva (L3) and immunomodulatory effects on Th9 cells, IL-9 immune response and TGF-β/Smad signaling regulator were evaluated at 7, 10, 14, 18, 21, 28 Days Post Infection (DPI). Flow cytometry was performed to evaluate the effects on Th9 cells and quantitative real time polymerase chain reaction was performed to evaluate the IL-9 cytokine transcription level. Additionally, fecal egg counting was also performed in parallel to confirm the infection. All goats were dewormed at 29 DPI and all experiments were also performed at 35 DPI, one week post deworming. The finding indicated that 10, 20, 40, 80 μg/mL concentration of ESPs incubated with goat PBMCs showed significant increase in the production of Th9 cells, signature cytokine IL-9 and expression of TGF-β/Smad signaling regulator as compared to control group in vitro.All infected groups showed significant increase in production of Th9 cells and IL-9 cytokine and expression of TGF-β/Smad key genes at 18, 21, and 28 DPI as compared to control group. Likewise, at 14 DPI, P-2400 and P-8000 groups showed significant increase in production of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 4 e0008218 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Muhammad Ali Memon Muhammad Ali-Ul-Husnain Naqvi Huang Xin Liang Meng Muhammad Waqqas Hasan Muhammad Haseeb Shakeel Ahmed Lakho Kalilixiati Aimulajiang Yongqian Bu Lixin Xu Xiaokai Song Xiangrui Li Ruofeng Yan Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
CD4+ T cells play critical roles in mediating adaptive immunity to a variety of pathogens. Recently, new subset of CD4+T named as T helper 9 cells that express the prototypical interleukin-9 (IL-9) cytokine have been recognized in human and mice models during different parasitic infections. Haemonchus contortus is a gastrointestinal nematode of small ruminants which cause high mortality in young animals. During infection, Excretory and Secretary Products (ESPs) are released in the host body. No other study has reported yet on immunomodulatory dynamics of H. contortus ESPs on Th9 immune response in vitro or in vivo. In this study, immunomodulatory effects of ESPs (5, 10, 20, 40, 80; μg/mL) incubated with goat PBMCs on Th9 cells, IL-9 immune response and TGF-β/Smad signaling regulator were evaluated in vitro. Moreover, for in vivo study, goats were infected with different doses (P-800, P-2400, and P-8000) of H. contortus infective larva (L3) and immunomodulatory effects on Th9 cells, IL-9 immune response and TGF-β/Smad signaling regulator were evaluated at 7, 10, 14, 18, 21, 28 Days Post Infection (DPI). Flow cytometry was performed to evaluate the effects on Th9 cells and quantitative real time polymerase chain reaction was performed to evaluate the IL-9 cytokine transcription level. Additionally, fecal egg counting was also performed in parallel to confirm the infection. All goats were dewormed at 29 DPI and all experiments were also performed at 35 DPI, one week post deworming. The finding indicated that 10, 20, 40, 80 μg/mL concentration of ESPs incubated with goat PBMCs showed significant increase in the production of Th9 cells, signature cytokine IL-9 and expression of TGF-β/Smad signaling regulator as compared to control group in vitro.All infected groups showed significant increase in production of Th9 cells and IL-9 cytokine and expression of TGF-β/Smad key genes at 18, 21, and 28 DPI as compared to control group. Likewise, at 14 DPI, P-2400 and P-8000 groups showed significant increase in production of ... |
format |
Article in Journal/Newspaper |
author |
Muhammad Ali Memon Muhammad Ali-Ul-Husnain Naqvi Huang Xin Liang Meng Muhammad Waqqas Hasan Muhammad Haseeb Shakeel Ahmed Lakho Kalilixiati Aimulajiang Yongqian Bu Lixin Xu Xiaokai Song Xiangrui Li Ruofeng Yan |
author_facet |
Muhammad Ali Memon Muhammad Ali-Ul-Husnain Naqvi Huang Xin Liang Meng Muhammad Waqqas Hasan Muhammad Haseeb Shakeel Ahmed Lakho Kalilixiati Aimulajiang Yongqian Bu Lixin Xu Xiaokai Song Xiangrui Li Ruofeng Yan |
author_sort |
Muhammad Ali Memon |
title |
Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat. |
title_short |
Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat. |
title_full |
Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat. |
title_fullStr |
Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat. |
title_full_unstemmed |
Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat. |
title_sort |
immunomodulatory dynamics of excretory and secretory products on th9 immune response during haemonchus contortus infection in goat. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008218 https://doaj.org/article/bd814cd2fb3c4643affddb8565ef498b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008218 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008218 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008218 https://doaj.org/article/bd814cd2fb3c4643affddb8565ef498b |
op_doi |
https://doi.org/10.1371/journal.pntd.0008218 |
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PLOS Neglected Tropical Diseases |
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14 |
container_issue |
4 |
container_start_page |
e0008218 |
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1766348123579351040 |