Profiles of small non-coding RNAs in Schistosoma japonicum during development.
BACKGROUND: The gene regulation mechanism along the life cycle of the genus Schistosoma is complex. Small non-coding RNAs (sncRNAs) are essential post transcriptional gene regulation elements that affect gene expression and mRNA stability. Preliminary studies indicated that sncRNAs in schistosomal p...
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ftdoajarticles:oai:doaj.org/article:bd4fea009fa14389989e0814494a4fa2 2023-05-15T15:12:12+02:00 Profiles of small non-coding RNAs in Schistosoma japonicum during development. Pengfei Cai Nan Hou Xianyu Piao Shuai Liu Haiying Liu Fan Yang Jianwei Wang Qi Jin Heng Wang Qijun Chen 2011-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001256 https://doaj.org/article/bd4fea009fa14389989e0814494a4fa2 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3149011?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001256 https://doaj.org/article/bd4fea009fa14389989e0814494a4fa2 PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1256 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001256 2022-12-31T14:38:00Z BACKGROUND: The gene regulation mechanism along the life cycle of the genus Schistosoma is complex. Small non-coding RNAs (sncRNAs) are essential post transcriptional gene regulation elements that affect gene expression and mRNA stability. Preliminary studies indicated that sncRNAs in schistosomal parasites are generated through different pathways, which are developmentally regulated. However, the data of sncRNAs of schistosomal parasites are still fragmental and a complete expression profile of sncRNAs during the parasite development requires a deep investigation. METHODOLOGY/PRINCIPAL FINDINGS: We employed high-throughput genome-wide transcriptome analytic techniques to explore the dynamic expression of microRNAs (miRNAs) and endogenous siRNAs (endo-siRNAs) of Schistosoma japonicum covering the free-living cercarial stage and all stages in the definitive host. This led us to analyze over 70 million clean reads represented both high and low abundance of the small RNA population. Patterns of differential expression of miRNAs and endo-siRNAs were observed. MiRNAs was twice more than endo-siRNAs in cercariae, but gradually decreased along with the development of the parasite. Both small RNA types were presented in equal aboudance in lung-stage schistosomula, while endo-siRNAs accumulated to 6 times more than miRNAs in adult female worms and hepatic eggs. Further, miRNAs were found mainly derived from genes located in the intergenic regions, while endo-siRNAs were mainly generated from transposable elements (TEs). The expression pattern of TE-siRNAs, as well as the pseudogene-derived siRNAs clustered in mRNAs of cytoskeletal proteins, stress proteins, enzymes related to energy metabolism also revealed distinction throughout different developmental stages. Natural antisense transcripts (NATs)-related siRNAs accounted for minor proportion of the endo-siRNAs which were dominantly expressed in cercariae. CONCLUSIONS/SIGNIFICANCE: Our results represented a comprehensive expression profile of sncRNAs in various ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 8 e1256 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Pengfei Cai Nan Hou Xianyu Piao Shuai Liu Haiying Liu Fan Yang Jianwei Wang Qi Jin Heng Wang Qijun Chen Profiles of small non-coding RNAs in Schistosoma japonicum during development. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: The gene regulation mechanism along the life cycle of the genus Schistosoma is complex. Small non-coding RNAs (sncRNAs) are essential post transcriptional gene regulation elements that affect gene expression and mRNA stability. Preliminary studies indicated that sncRNAs in schistosomal parasites are generated through different pathways, which are developmentally regulated. However, the data of sncRNAs of schistosomal parasites are still fragmental and a complete expression profile of sncRNAs during the parasite development requires a deep investigation. METHODOLOGY/PRINCIPAL FINDINGS: We employed high-throughput genome-wide transcriptome analytic techniques to explore the dynamic expression of microRNAs (miRNAs) and endogenous siRNAs (endo-siRNAs) of Schistosoma japonicum covering the free-living cercarial stage and all stages in the definitive host. This led us to analyze over 70 million clean reads represented both high and low abundance of the small RNA population. Patterns of differential expression of miRNAs and endo-siRNAs were observed. MiRNAs was twice more than endo-siRNAs in cercariae, but gradually decreased along with the development of the parasite. Both small RNA types were presented in equal aboudance in lung-stage schistosomula, while endo-siRNAs accumulated to 6 times more than miRNAs in adult female worms and hepatic eggs. Further, miRNAs were found mainly derived from genes located in the intergenic regions, while endo-siRNAs were mainly generated from transposable elements (TEs). The expression pattern of TE-siRNAs, as well as the pseudogene-derived siRNAs clustered in mRNAs of cytoskeletal proteins, stress proteins, enzymes related to energy metabolism also revealed distinction throughout different developmental stages. Natural antisense transcripts (NATs)-related siRNAs accounted for minor proportion of the endo-siRNAs which were dominantly expressed in cercariae. CONCLUSIONS/SIGNIFICANCE: Our results represented a comprehensive expression profile of sncRNAs in various ... |
format |
Article in Journal/Newspaper |
author |
Pengfei Cai Nan Hou Xianyu Piao Shuai Liu Haiying Liu Fan Yang Jianwei Wang Qi Jin Heng Wang Qijun Chen |
author_facet |
Pengfei Cai Nan Hou Xianyu Piao Shuai Liu Haiying Liu Fan Yang Jianwei Wang Qi Jin Heng Wang Qijun Chen |
author_sort |
Pengfei Cai |
title |
Profiles of small non-coding RNAs in Schistosoma japonicum during development. |
title_short |
Profiles of small non-coding RNAs in Schistosoma japonicum during development. |
title_full |
Profiles of small non-coding RNAs in Schistosoma japonicum during development. |
title_fullStr |
Profiles of small non-coding RNAs in Schistosoma japonicum during development. |
title_full_unstemmed |
Profiles of small non-coding RNAs in Schistosoma japonicum during development. |
title_sort |
profiles of small non-coding rnas in schistosoma japonicum during development. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001256 https://doaj.org/article/bd4fea009fa14389989e0814494a4fa2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1256 (2011) |
op_relation |
http://europepmc.org/articles/PMC3149011?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001256 https://doaj.org/article/bd4fea009fa14389989e0814494a4fa2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001256 |
container_title |
PLoS Neglected Tropical Diseases |
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5 |
container_issue |
8 |
container_start_page |
e1256 |
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1766342916203085824 |