Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut.
BACKGROUND:Trypanosoma cruzi is the etiological agent of Chagas' disease. Cysteine peptidases are relevant to several aspects of the T. cruzi life cycle and are implicated in parasite-mammalian host relationships. However, little is known about the factors that contribute to the parasite-insect...
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Online Access: | https://doi.org/10.1371/journal.pntd.0001958 https://doaj.org/article/bd3b1bd32fb649d6a199667b4ed5570a |
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ftdoajarticles:oai:doaj.org/article:bd3b1bd32fb649d6a199667b4ed5570a 2023-05-15T15:12:25+02:00 Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. Lívia Almeida Uehara Otacílio C Moreira Ana Carolina Oliveira Patrícia Azambuja Ana Paula Cabral Araujo Lima Constança Britto André Luis Souza dos Santos Marta Helena Branquinha Claudia Masini d'Avila-Levy 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001958 https://doaj.org/article/bd3b1bd32fb649d6a199667b4ed5570a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3521651?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001958 https://doaj.org/article/bd3b1bd32fb649d6a199667b4ed5570a PLoS Neglected Tropical Diseases, Vol 6, Iss 12, p e1958 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001958 2022-12-31T11:40:40Z BACKGROUND:Trypanosoma cruzi is the etiological agent of Chagas' disease. Cysteine peptidases are relevant to several aspects of the T. cruzi life cycle and are implicated in parasite-mammalian host relationships. However, little is known about the factors that contribute to the parasite-insect host interaction. METHODOLOGY/PRINCIPAL FINDINGS:Here, we have investigated whether cruzipain could be involved in the interaction of T. cruzi with the invertebrate host. We analyzed the effect of treatment of T. cruzi epimastigotes with anti-cruzipain antibodies or with a panel of cysteine peptidase inhibitors (cystatin, antipain, E-64, leupeptin, iodocetamide or CA-074-OMe) on parasite adhesion to Rhodnius prolixus posterior midgut ex vivo. All treatments, with the exception of CA074-OMe, significantly decreased parasite adhesion to R. prolixus midgut. Cystatin presented a dose-dependent reduction on the adhesion. Comparison of the adhesion rate among several T. cruzi isolates revealed that the G isolate, which naturally possesses low levels of active cruzipain, adhered to a lesser extent in comparison to Dm28c, Y and CL Brener isolates. Transgenic epimastigotes overexpressing an endogenous cruzipain inhibitor (pCHAG), chagasin, and that have reduced levels of active cruzipain adhered to the insect gut 73% less than the wild-type parasites. The adhesion of pCHAG parasites was partially restored by the addition of exogenous cruzipain. In vivo colonization experiments revealed low levels of pCHAG parasites in comparison to wild-type. Parasites isolated after passage in the insect presented a drastic enhancement in the expression of surface cruzipain. CONCLUSIONS/SIGNIFICANCE:These data highlight, for the first time, that cruzipain contributes to the interaction of T. cruzi with the insect host. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 12 e1958 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lívia Almeida Uehara Otacílio C Moreira Ana Carolina Oliveira Patrícia Azambuja Ana Paula Cabral Araujo Lima Constança Britto André Luis Souza dos Santos Marta Helena Branquinha Claudia Masini d'Avila-Levy Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Trypanosoma cruzi is the etiological agent of Chagas' disease. Cysteine peptidases are relevant to several aspects of the T. cruzi life cycle and are implicated in parasite-mammalian host relationships. However, little is known about the factors that contribute to the parasite-insect host interaction. METHODOLOGY/PRINCIPAL FINDINGS:Here, we have investigated whether cruzipain could be involved in the interaction of T. cruzi with the invertebrate host. We analyzed the effect of treatment of T. cruzi epimastigotes with anti-cruzipain antibodies or with a panel of cysteine peptidase inhibitors (cystatin, antipain, E-64, leupeptin, iodocetamide or CA-074-OMe) on parasite adhesion to Rhodnius prolixus posterior midgut ex vivo. All treatments, with the exception of CA074-OMe, significantly decreased parasite adhesion to R. prolixus midgut. Cystatin presented a dose-dependent reduction on the adhesion. Comparison of the adhesion rate among several T. cruzi isolates revealed that the G isolate, which naturally possesses low levels of active cruzipain, adhered to a lesser extent in comparison to Dm28c, Y and CL Brener isolates. Transgenic epimastigotes overexpressing an endogenous cruzipain inhibitor (pCHAG), chagasin, and that have reduced levels of active cruzipain adhered to the insect gut 73% less than the wild-type parasites. The adhesion of pCHAG parasites was partially restored by the addition of exogenous cruzipain. In vivo colonization experiments revealed low levels of pCHAG parasites in comparison to wild-type. Parasites isolated after passage in the insect presented a drastic enhancement in the expression of surface cruzipain. CONCLUSIONS/SIGNIFICANCE:These data highlight, for the first time, that cruzipain contributes to the interaction of T. cruzi with the insect host. |
format |
Article in Journal/Newspaper |
author |
Lívia Almeida Uehara Otacílio C Moreira Ana Carolina Oliveira Patrícia Azambuja Ana Paula Cabral Araujo Lima Constança Britto André Luis Souza dos Santos Marta Helena Branquinha Claudia Masini d'Avila-Levy |
author_facet |
Lívia Almeida Uehara Otacílio C Moreira Ana Carolina Oliveira Patrícia Azambuja Ana Paula Cabral Araujo Lima Constança Britto André Luis Souza dos Santos Marta Helena Branquinha Claudia Masini d'Avila-Levy |
author_sort |
Lívia Almeida Uehara |
title |
Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. |
title_short |
Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. |
title_full |
Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. |
title_fullStr |
Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. |
title_full_unstemmed |
Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. |
title_sort |
cruzipain promotes trypanosoma cruzi adhesion to rhodnius prolixus midgut. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001958 https://doaj.org/article/bd3b1bd32fb649d6a199667b4ed5570a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 12, p e1958 (2012) |
op_relation |
http://europepmc.org/articles/PMC3521651?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001958 https://doaj.org/article/bd3b1bd32fb649d6a199667b4ed5570a |
op_doi |
https://doi.org/10.1371/journal.pntd.0001958 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
12 |
container_start_page |
e1958 |
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1766343094748315648 |