Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice
Objective: To examine the effect of Pandanus amaryllifolius (P. amaryllifolius) leaf extract on the insulin resistance state in obese ICR mice. Methods: Obesity was induced in mice fed with high-fat diet (45% fat) for 12 weeks. After the first six weeks on the diet, the obese mice were administered...
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Wolters Kluwer Medknow Publications
2016
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ftdoajarticles:oai:doaj.org/article:bd28462668cf4678bd7ea95dea9ae7f5 2023-05-15T15:11:56+02:00 Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice Suphaket Saenthaweesuk Jarinyaporn Naowaboot Nuntiya Somparn 2016-10-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.08.010 https://doaj.org/article/bd28462668cf4678bd7ea95dea9ae7f5 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116300594 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.08.010 https://doaj.org/article/bd28462668cf4678bd7ea95dea9ae7f5 Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 10, Pp 866-871 (2016) Pandanus amaryllifolius Obesity Insulin sensitivity Fatty liver Glucose transporter 4 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.08.010 2022-12-31T08:49:05Z Objective: To examine the effect of Pandanus amaryllifolius (P. amaryllifolius) leaf extract on the insulin resistance state in obese ICR mice. Methods: Obesity was induced in mice fed with high-fat diet (45% fat) for 12 weeks. After the first six weeks on the diet, the obese mice were administered with the water extract of P. amaryllifolius leaf at 125 and 250 mg/kg/day, respectively for another six weeks. At the 5th week of treatment, oral glucose tolerance test was conducted. After six weeks of treatment, the levels of blood glucose, serum insulin, leptin, adiponectin, and lipid profiles were determined. The liver, muscle and epididymal fat tissues were removed for measuring the biochemical parameters and protein expression, as well as histological examination. Results: Six weeks of treatment with P. amaryllifolius led to a significant reduction in the blood glucose level as well as improvement in the insulin resistance. P. amaryllifolius also increased the liver glycogen storage and serum adiponectin and decreased the serum leptin levels. A reduction in the serum and hepatic triglyceride, and non-esterified fatty acid levels was also observed. The histological examination showed that the obese mice treated with P. amaryllifolius reduced the lipid droplet in liver tissue and adipocyte size in epididymal fat tissue. The treatment also increased the protein expression of glucose transporter 4 in the muscle and fat tissues. Conclusions: The treatment with P. amaryllifolius could decrease several parameters of impaired glucose and lipid metabolism. To the best of our knowledge, this is the first report on the role of P. amaryllifolius leaf extract in alleviating the insulin dysfunction in obesity state. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 10 866 871 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pandanus amaryllifolius Obesity Insulin sensitivity Fatty liver Glucose transporter 4 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Pandanus amaryllifolius Obesity Insulin sensitivity Fatty liver Glucose transporter 4 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Suphaket Saenthaweesuk Jarinyaporn Naowaboot Nuntiya Somparn Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice |
topic_facet |
Pandanus amaryllifolius Obesity Insulin sensitivity Fatty liver Glucose transporter 4 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To examine the effect of Pandanus amaryllifolius (P. amaryllifolius) leaf extract on the insulin resistance state in obese ICR mice. Methods: Obesity was induced in mice fed with high-fat diet (45% fat) for 12 weeks. After the first six weeks on the diet, the obese mice were administered with the water extract of P. amaryllifolius leaf at 125 and 250 mg/kg/day, respectively for another six weeks. At the 5th week of treatment, oral glucose tolerance test was conducted. After six weeks of treatment, the levels of blood glucose, serum insulin, leptin, adiponectin, and lipid profiles were determined. The liver, muscle and epididymal fat tissues were removed for measuring the biochemical parameters and protein expression, as well as histological examination. Results: Six weeks of treatment with P. amaryllifolius led to a significant reduction in the blood glucose level as well as improvement in the insulin resistance. P. amaryllifolius also increased the liver glycogen storage and serum adiponectin and decreased the serum leptin levels. A reduction in the serum and hepatic triglyceride, and non-esterified fatty acid levels was also observed. The histological examination showed that the obese mice treated with P. amaryllifolius reduced the lipid droplet in liver tissue and adipocyte size in epididymal fat tissue. The treatment also increased the protein expression of glucose transporter 4 in the muscle and fat tissues. Conclusions: The treatment with P. amaryllifolius could decrease several parameters of impaired glucose and lipid metabolism. To the best of our knowledge, this is the first report on the role of P. amaryllifolius leaf extract in alleviating the insulin dysfunction in obesity state. |
format |
Article in Journal/Newspaper |
author |
Suphaket Saenthaweesuk Jarinyaporn Naowaboot Nuntiya Somparn |
author_facet |
Suphaket Saenthaweesuk Jarinyaporn Naowaboot Nuntiya Somparn |
author_sort |
Suphaket Saenthaweesuk |
title |
Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice |
title_short |
Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice |
title_full |
Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice |
title_fullStr |
Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice |
title_full_unstemmed |
Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice |
title_sort |
pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.08.010 https://doaj.org/article/bd28462668cf4678bd7ea95dea9ae7f5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 10, Pp 866-871 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116300594 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.08.010 https://doaj.org/article/bd28462668cf4678bd7ea95dea9ae7f5 |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.08.010 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
10 |
container_start_page |
866 |
op_container_end_page |
871 |
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