Exploratory RNA-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement
Exposure to stressful environments weakens immunity evidenced by a detectable reactivation of dormant viruses. The mechanism behind this observation remains unclear. We performed next generation sequencing from RNA extracted from blood samples of 8 male subjects collected before, during and after a...
Published in: | Brain, Behavior, & Immunity - Health |
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ftdoajarticles:oai:doaj.org/article:bd1ac6f82b5a40e7913c0c27bd01d203 2023-05-15T13:51:44+02:00 Exploratory RNA-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement Judith-Irina Buchheim Jean-Noël Billaud Matthias Feuerecker Claudia Strewe Carole Dangoisse Andreas Osterman Satish Mehta Brian Crucian Gustav Schelling Alexander Choukér 2020-12-01T00:00:00Z https://doi.org/10.1016/j.bbih.2020.100145 https://doaj.org/article/bd1ac6f82b5a40e7913c0c27bd01d203 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2666354620301101 https://doaj.org/toc/2666-3546 2666-3546 doi:10.1016/j.bbih.2020.100145 https://doaj.org/article/bd1ac6f82b5a40e7913c0c27bd01d203 Brain, Behavior, & Immunity - Health, Vol 9, Iss , Pp 100145- (2020) Stress Isolation Confinement RNA-seq Antiviral immunity Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 article 2020 ftdoajarticles https://doi.org/10.1016/j.bbih.2020.100145 2022-12-31T07:58:32Z Exposure to stressful environments weakens immunity evidenced by a detectable reactivation of dormant viruses. The mechanism behind this observation remains unclear. We performed next generation sequencing from RNA extracted from blood samples of 8 male subjects collected before, during and after a 12-month stay at the Antarctic station Concordia. RNA-seq data analysis was done using QIAGEN Ingenuity Pathway Analysis (IPA) software. Data revealed the inactivation of key immune functions such as chemotaxis and leukocyte recruitment which persisted after return. Next to the activation of the stress response eIF2 pathway, interferon signaling was predicted inactivated due to a downregulation of 14 downstream genes involved in antiviral immunity. Among them, the interferon stimulated genes (ISGs) IFITM2 and 3 as well as IFIT3 exhibited the strongest fold changes and IFIT3 remained downregulated even after return. Impairment of antiviral immunity in winter-over crew can be explained by the downregulation of a battery of ISGs. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Brain, Behavior, & Immunity - Health 9 100145 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Stress Isolation Confinement RNA-seq Antiviral immunity Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 |
spellingShingle |
Stress Isolation Confinement RNA-seq Antiviral immunity Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Judith-Irina Buchheim Jean-Noël Billaud Matthias Feuerecker Claudia Strewe Carole Dangoisse Andreas Osterman Satish Mehta Brian Crucian Gustav Schelling Alexander Choukér Exploratory RNA-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement |
topic_facet |
Stress Isolation Confinement RNA-seq Antiviral immunity Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 |
description |
Exposure to stressful environments weakens immunity evidenced by a detectable reactivation of dormant viruses. The mechanism behind this observation remains unclear. We performed next generation sequencing from RNA extracted from blood samples of 8 male subjects collected before, during and after a 12-month stay at the Antarctic station Concordia. RNA-seq data analysis was done using QIAGEN Ingenuity Pathway Analysis (IPA) software. Data revealed the inactivation of key immune functions such as chemotaxis and leukocyte recruitment which persisted after return. Next to the activation of the stress response eIF2 pathway, interferon signaling was predicted inactivated due to a downregulation of 14 downstream genes involved in antiviral immunity. Among them, the interferon stimulated genes (ISGs) IFITM2 and 3 as well as IFIT3 exhibited the strongest fold changes and IFIT3 remained downregulated even after return. Impairment of antiviral immunity in winter-over crew can be explained by the downregulation of a battery of ISGs. |
format |
Article in Journal/Newspaper |
author |
Judith-Irina Buchheim Jean-Noël Billaud Matthias Feuerecker Claudia Strewe Carole Dangoisse Andreas Osterman Satish Mehta Brian Crucian Gustav Schelling Alexander Choukér |
author_facet |
Judith-Irina Buchheim Jean-Noël Billaud Matthias Feuerecker Claudia Strewe Carole Dangoisse Andreas Osterman Satish Mehta Brian Crucian Gustav Schelling Alexander Choukér |
author_sort |
Judith-Irina Buchheim |
title |
Exploratory RNA-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement |
title_short |
Exploratory RNA-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement |
title_full |
Exploratory RNA-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement |
title_fullStr |
Exploratory RNA-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement |
title_full_unstemmed |
Exploratory RNA-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement |
title_sort |
exploratory rna-seq analysis in healthy subjects reveals vulnerability to viral infections during a 12- month period of isolation and confinement |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.bbih.2020.100145 https://doaj.org/article/bd1ac6f82b5a40e7913c0c27bd01d203 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Brain, Behavior, & Immunity - Health, Vol 9, Iss , Pp 100145- (2020) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2666354620301101 https://doaj.org/toc/2666-3546 2666-3546 doi:10.1016/j.bbih.2020.100145 https://doaj.org/article/bd1ac6f82b5a40e7913c0c27bd01d203 |
op_doi |
https://doi.org/10.1016/j.bbih.2020.100145 |
container_title |
Brain, Behavior, & Immunity - Health |
container_volume |
9 |
container_start_page |
100145 |
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1766255765769682944 |