Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP
Abstract Background Anopheles longipalpis is morphologically similar to the major African malaria vector Anopheles funestus at the adult stage although it is very different at the larval stage. Despite the development of the species-specific multiplex PCR assay for the An. funestus group, the genomi...
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ftdoajarticles:oai:doaj.org/article:bcc7f4c73f7b4c12bb12034a5e4a29e3 2023-05-15T15:18:21+02:00 Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP Coetzee Maureen Choi Kwang Koekemoer Lizette L 2010-11-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-316 https://doaj.org/article/bcc7f4c73f7b4c12bb12034a5e4a29e3 EN eng BMC http://www.malariajournal.com/content/9/1/316 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-316 1475-2875 https://doaj.org/article/bcc7f4c73f7b4c12bb12034a5e4a29e3 Malaria Journal, Vol 9, Iss 1, p 316 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-316 2022-12-31T04:13:01Z Abstract Background Anopheles longipalpis is morphologically similar to the major African malaria vector Anopheles funestus at the adult stage although it is very different at the larval stage. Despite the development of the species-specific multiplex PCR assay for the An. funestus group, the genomic DNA of Anopheles longipalpis type C specimens can be amplified with the Anopheles vaneedeni and Anopheles parensis primers from this assay. The standard, species-specific An. funestus group PCR, results in the amplification of two fragments when An. longipalpis type C specimens are included in the analysis. This result can easily be misinterpreted as being a hybrid between An. vaneedeni and An. parensis . Anopheles longipalpis type C can be identified using a species-specific PCR assay but this assay is not reliable if other members of the An. funestus group, such as An. funestus , An. funestus -like and An. parensis , are included. The present study provides a multiplex assay that will identify An. longipalpis along with other common members of the African An. funestus group, including Anopheles leesoni . Methods A total of 70 specimens from six species ( An. funestus , An. funestus -like, An. parensis , Anopheles rivulorum , An. vaneedeni and An. leesoni ) in the An. funestus group and An. longipalpis type C from Malawi, Mozambique, South Africa and Zambia were used for the study. A restriction fragment length polymorphism (RFLP) assay was designed based on the DNA sequence information in the GenBank database. Results The enzyme, EcoRI digested only An. longipalpis type C and An. funestus -like after the species-specific An. funestus group PCR assay. The An. longipalpis and An. funestus -like digestion profiles were characterized by three fragments, 376 bp, 252 bp and 211 bp for An. longipalpis type C and two fragments, 375 bp and 15 bp for An. funestus -like. Conclusions An RFLP method for the group was developed that is more accurate and efficient than those used before. Hence, this assay would be useful for ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 316 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Coetzee Maureen Choi Kwang Koekemoer Lizette L Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Anopheles longipalpis is morphologically similar to the major African malaria vector Anopheles funestus at the adult stage although it is very different at the larval stage. Despite the development of the species-specific multiplex PCR assay for the An. funestus group, the genomic DNA of Anopheles longipalpis type C specimens can be amplified with the Anopheles vaneedeni and Anopheles parensis primers from this assay. The standard, species-specific An. funestus group PCR, results in the amplification of two fragments when An. longipalpis type C specimens are included in the analysis. This result can easily be misinterpreted as being a hybrid between An. vaneedeni and An. parensis . Anopheles longipalpis type C can be identified using a species-specific PCR assay but this assay is not reliable if other members of the An. funestus group, such as An. funestus , An. funestus -like and An. parensis , are included. The present study provides a multiplex assay that will identify An. longipalpis along with other common members of the African An. funestus group, including Anopheles leesoni . Methods A total of 70 specimens from six species ( An. funestus , An. funestus -like, An. parensis , Anopheles rivulorum , An. vaneedeni and An. leesoni ) in the An. funestus group and An. longipalpis type C from Malawi, Mozambique, South Africa and Zambia were used for the study. A restriction fragment length polymorphism (RFLP) assay was designed based on the DNA sequence information in the GenBank database. Results The enzyme, EcoRI digested only An. longipalpis type C and An. funestus -like after the species-specific An. funestus group PCR assay. The An. longipalpis and An. funestus -like digestion profiles were characterized by three fragments, 376 bp, 252 bp and 211 bp for An. longipalpis type C and two fragments, 375 bp and 15 bp for An. funestus -like. Conclusions An RFLP method for the group was developed that is more accurate and efficient than those used before. Hence, this assay would be useful for ... |
format |
Article in Journal/Newspaper |
author |
Coetzee Maureen Choi Kwang Koekemoer Lizette L |
author_facet |
Coetzee Maureen Choi Kwang Koekemoer Lizette L |
author_sort |
Coetzee Maureen |
title |
Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP |
title_short |
Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP |
title_full |
Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP |
title_fullStr |
Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP |
title_full_unstemmed |
Simultaneous identification of the Anopheles funestus group and Anopheles longipalpis type C by PCR-RFLP |
title_sort |
simultaneous identification of the anopheles funestus group and anopheles longipalpis type c by pcr-rflp |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-316 https://doaj.org/article/bcc7f4c73f7b4c12bb12034a5e4a29e3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 316 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/316 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-316 1475-2875 https://doaj.org/article/bcc7f4c73f7b4c12bb12034a5e4a29e3 |
op_doi |
https://doi.org/10.1186/1475-2875-9-316 |
container_title |
Malaria Journal |
container_volume |
9 |
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1 |
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316 |
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1766348551132020736 |