Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile
Abstract Background Genetic polymophisms of the Duffy antigen receptor for the chemokines (DARC) gene successfully protected against blood stage infection by Plasmodium vivax infection. The Fy (a-, b-) phenotype is predominant among African populations, particularly those originating from West Afric...
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ftdoajarticles:oai:doaj.org/article:bcb5b9852ffc46159ace8aaa47057d98 2023-05-15T15:19:10+02:00 Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile Kempińska-Podhorodecka Agnieszka Knap Oktawian Drozd Arleta Kaczmarczyk Mariusz Parafiniuk Miroslaw Parczewski Milosz Ciechanowicz Andrzej 2012-04-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-115 https://doaj.org/article/bcb5b9852ffc46159ace8aaa47057d98 EN eng BMC http://www.malariajournal.com/content/11/1/115 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-115 1475-2875 https://doaj.org/article/bcb5b9852ffc46159ace8aaa47057d98 Malaria Journal, Vol 11, Iss 1, p 115 (2012) Duffy blood group Malaria Sudanese Arabs Shagia Manasir SNP polymorphism Real-Time PCR Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-115 2022-12-31T11:51:58Z Abstract Background Genetic polymophisms of the Duffy antigen receptor for the chemokines (DARC) gene successfully protected against blood stage infection by Plasmodium vivax infection. The Fy (a-, b-) phenotype is predominant among African populations, particularly those originating from West Africa, and it is rare among non-African populations. The aim of this study was to analyse the frequency of four Duffy blood groups based on SNPs (T-33C, G125A, G298A and C5411T) in two local tribes of Sudanese Arabs, the Shagia and Manasir , which are both from the region of the Fourth Nile cataract in Sudan. Methods An analysis of polymorphisms was performed on 217 individuals (126 representatives of the Shagia tribe and 91 of the Manasir) . Real-time PCR and TaqMan Genotyping Assays were used to study the prevalence of alleles and genotypes. Results The analysis of allelic and genotype frequency in the T-33C polymorphisms demonstrated a significant dominance of the C allele and CC genotype (OR = 0.53 [0.32-0.88]; p = 0.02) in both tribes. The G125A polymorphism is associated with phenotype Fy(a-, b-) and was identified in 83% of Shagia and 77% of Manasir . With regard to G298A polymorphisms, the genotype frequencies were different between the tribes (p = 0,002) and no single AA homozygote was found. Based on four SNPs examined, 20 combinations of genotypes for the Shagia and Manasir tribes were determined. The genotype CC/AA/GG/CT occurred most often in Shagia tribe (45.9%) but was rare in the Manasir tribe (6.6%) (p < 0.001 Shagia versus Manasir ). The FY*A ES allele was identified in both analysed tribes. The presence of individuals with the FY*A/FY*A genotype was demonstrated only in the Shagia tribe. Conclusion This is probably the first report showing genotypically Duffy-negative people who carry both FY*B ES and FY*A ES . The identification of the FY*A ES allele in both tribes may be due to admixture of the non-African genetic background. Taken as a whole, allele and genotype frequencies between the Shagia and ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 |
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English |
topic |
Duffy blood group Malaria Sudanese Arabs Shagia Manasir SNP polymorphism Real-Time PCR Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Duffy blood group Malaria Sudanese Arabs Shagia Manasir SNP polymorphism Real-Time PCR Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Kempińska-Podhorodecka Agnieszka Knap Oktawian Drozd Arleta Kaczmarczyk Mariusz Parafiniuk Miroslaw Parczewski Milosz Ciechanowicz Andrzej Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile |
topic_facet |
Duffy blood group Malaria Sudanese Arabs Shagia Manasir SNP polymorphism Real-Time PCR Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Genetic polymophisms of the Duffy antigen receptor for the chemokines (DARC) gene successfully protected against blood stage infection by Plasmodium vivax infection. The Fy (a-, b-) phenotype is predominant among African populations, particularly those originating from West Africa, and it is rare among non-African populations. The aim of this study was to analyse the frequency of four Duffy blood groups based on SNPs (T-33C, G125A, G298A and C5411T) in two local tribes of Sudanese Arabs, the Shagia and Manasir , which are both from the region of the Fourth Nile cataract in Sudan. Methods An analysis of polymorphisms was performed on 217 individuals (126 representatives of the Shagia tribe and 91 of the Manasir) . Real-time PCR and TaqMan Genotyping Assays were used to study the prevalence of alleles and genotypes. Results The analysis of allelic and genotype frequency in the T-33C polymorphisms demonstrated a significant dominance of the C allele and CC genotype (OR = 0.53 [0.32-0.88]; p = 0.02) in both tribes. The G125A polymorphism is associated with phenotype Fy(a-, b-) and was identified in 83% of Shagia and 77% of Manasir . With regard to G298A polymorphisms, the genotype frequencies were different between the tribes (p = 0,002) and no single AA homozygote was found. Based on four SNPs examined, 20 combinations of genotypes for the Shagia and Manasir tribes were determined. The genotype CC/AA/GG/CT occurred most often in Shagia tribe (45.9%) but was rare in the Manasir tribe (6.6%) (p < 0.001 Shagia versus Manasir ). The FY*A ES allele was identified in both analysed tribes. The presence of individuals with the FY*A/FY*A genotype was demonstrated only in the Shagia tribe. Conclusion This is probably the first report showing genotypically Duffy-negative people who carry both FY*B ES and FY*A ES . The identification of the FY*A ES allele in both tribes may be due to admixture of the non-African genetic background. Taken as a whole, allele and genotype frequencies between the Shagia and ... |
format |
Article in Journal/Newspaper |
author |
Kempińska-Podhorodecka Agnieszka Knap Oktawian Drozd Arleta Kaczmarczyk Mariusz Parafiniuk Miroslaw Parczewski Milosz Ciechanowicz Andrzej |
author_facet |
Kempińska-Podhorodecka Agnieszka Knap Oktawian Drozd Arleta Kaczmarczyk Mariusz Parafiniuk Miroslaw Parczewski Milosz Ciechanowicz Andrzej |
author_sort |
Kempińska-Podhorodecka Agnieszka |
title |
Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile |
title_short |
Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile |
title_full |
Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile |
title_fullStr |
Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile |
title_full_unstemmed |
Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile |
title_sort |
analysis for genotyping duffy blood group in inhabitants of sudan, the fourth cataract of the nile |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-115 https://doaj.org/article/bcb5b9852ffc46159ace8aaa47057d98 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 115 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/115 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-115 1475-2875 https://doaj.org/article/bcb5b9852ffc46159ace8aaa47057d98 |
op_doi |
https://doi.org/10.1186/1475-2875-11-115 |
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Malaria Journal |
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11 |
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