Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish
Objective: To examine and study the morphology, epidemiology, and molecular phylogeny of Anisakis larvae in blue mackerel [Scomber australasicus (Cuvier, 1832)] and Indian mackerel [Rastrelliger kanagurta (Cuvier, 1816)] using light microscope, scanning electron microscope, molecular phylogeny, and...
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Wolters Kluwer Medknow Publications
2021
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ftdoajarticles:oai:doaj.org/article:bc764d9fd0694d37bbb55b0d6135b18c 2023-05-15T15:11:13+02:00 Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish Vipavinee Cheypanya Pheravut Wongsawad Chalobol Wongsawad Nattawadee Nantarat 2021-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.315900 https://doaj.org/article/bc764d9fd0694d37bbb55b0d6135b18c EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=5;spage=214;epage=222;aulast=Cheypanya https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.315900 https://doaj.org/article/bc764d9fd0694d37bbb55b0d6135b18c Asian Pacific Journal of Tropical Medicine, Vol 14, Iss 5, Pp 214-222 (2021) anisakis mackerel thailand phylogenetic tree taxonomy species delimitation Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.4103/1995-7645.315900 2022-12-30T20:39:23Z Objective: To examine and study the morphology, epidemiology, and molecular phylogeny of Anisakis larvae in blue mackerel [Scomber australasicus (Cuvier, 1832)] and Indian mackerel [Rastrelliger kanagurta (Cuvier, 1816)] using light microscope, scanning electron microscope, molecular phylogeny, and species delimitation methods for confirmation and investigation of Anisakis species and their evolutionary relationship. Methods: A total of 90 fish (45 per species) were purchased from a department store in Chiang Mai, Thailand. Anisakis samples were investigated for morphological characteristics using light and scanning electron microscopes. Molecular phylogeny and species delimitation methods based on the cox2 gene were performed. Results: The prevalence, mean intensity (Mean±SEM), and mean abundance of Anisakis larvae (Mean±SEM) in blue mackerel were 77.78%, 6.74±1.320, and 5.24±1.107, respectively, and in Indian mackerel, these values were 13.33%, 2.50±0.764, and 0.33±0.159, respectively. Scanning electron microscopy showed the detail of morphological characteristics and provided the different shapes of mucron and excretory pores in Anisakis larvae congruent with the phylogenetic tree. The species tree was congruent with the phylogenetic tree. Conclusions: The prevalence, mean intensity, and mean abundance of Anisakis larvae were higher in blue mackerel. To the best of our knowledge, this is the first time that Anisakis pegreffii was found in blue mackerel in Thailand. The phylogenetic tree also supported the morphological data of Anisakis larvae. However, species delimitation based on cox2 revealed 1-3 possible cryptic species in this genus. Anisakis spp. contamination of fish products is unpleasant and a health concern considering human infection with larvae (anisakiasis) arises. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian Chiang ENVELOPE(162.650,162.650,-77.967,-77.967) Asian Pacific Journal of Tropical Medicine 14 5 214 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
anisakis mackerel thailand phylogenetic tree taxonomy species delimitation Arctic medicine. Tropical medicine RC955-962 |
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anisakis mackerel thailand phylogenetic tree taxonomy species delimitation Arctic medicine. Tropical medicine RC955-962 Vipavinee Cheypanya Pheravut Wongsawad Chalobol Wongsawad Nattawadee Nantarat Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish |
topic_facet |
anisakis mackerel thailand phylogenetic tree taxonomy species delimitation Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To examine and study the morphology, epidemiology, and molecular phylogeny of Anisakis larvae in blue mackerel [Scomber australasicus (Cuvier, 1832)] and Indian mackerel [Rastrelliger kanagurta (Cuvier, 1816)] using light microscope, scanning electron microscope, molecular phylogeny, and species delimitation methods for confirmation and investigation of Anisakis species and their evolutionary relationship. Methods: A total of 90 fish (45 per species) were purchased from a department store in Chiang Mai, Thailand. Anisakis samples were investigated for morphological characteristics using light and scanning electron microscopes. Molecular phylogeny and species delimitation methods based on the cox2 gene were performed. Results: The prevalence, mean intensity (Mean±SEM), and mean abundance of Anisakis larvae (Mean±SEM) in blue mackerel were 77.78%, 6.74±1.320, and 5.24±1.107, respectively, and in Indian mackerel, these values were 13.33%, 2.50±0.764, and 0.33±0.159, respectively. Scanning electron microscopy showed the detail of morphological characteristics and provided the different shapes of mucron and excretory pores in Anisakis larvae congruent with the phylogenetic tree. The species tree was congruent with the phylogenetic tree. Conclusions: The prevalence, mean intensity, and mean abundance of Anisakis larvae were higher in blue mackerel. To the best of our knowledge, this is the first time that Anisakis pegreffii was found in blue mackerel in Thailand. The phylogenetic tree also supported the morphological data of Anisakis larvae. However, species delimitation based on cox2 revealed 1-3 possible cryptic species in this genus. Anisakis spp. contamination of fish products is unpleasant and a health concern considering human infection with larvae (anisakiasis) arises. |
format |
Article in Journal/Newspaper |
author |
Vipavinee Cheypanya Pheravut Wongsawad Chalobol Wongsawad Nattawadee Nantarat |
author_facet |
Vipavinee Cheypanya Pheravut Wongsawad Chalobol Wongsawad Nattawadee Nantarat |
author_sort |
Vipavinee Cheypanya |
title |
Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish |
title_short |
Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish |
title_full |
Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish |
title_fullStr |
Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish |
title_full_unstemmed |
Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish |
title_sort |
morphological study and molecular epidemiology of anisakis larvae in mackerel fish |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/1995-7645.315900 https://doaj.org/article/bc764d9fd0694d37bbb55b0d6135b18c |
long_lat |
ENVELOPE(162.650,162.650,-77.967,-77.967) |
geographic |
Arctic Indian Chiang |
geographic_facet |
Arctic Indian Chiang |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 14, Iss 5, Pp 214-222 (2021) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=5;spage=214;epage=222;aulast=Cheypanya https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.315900 https://doaj.org/article/bc764d9fd0694d37bbb55b0d6135b18c |
op_doi |
https://doi.org/10.4103/1995-7645.315900 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
14 |
container_issue |
5 |
container_start_page |
214 |
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1766342104372477952 |