The Truman Show for protozoan parasites: A review of in vitro cultivation platforms.
Protozoan parasites are responsible for severe disease and suffering in humans worldwide. Apart from disease transmission via insect vectors and contaminated soil, food, or water, transmission may occur congenitally or by way of blood transfusion and organ transplantation. Several recent outbreaks a...
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ftdoajarticles:oai:doaj.org/article:bc7477298ff34a628e05a2215f459085 2023-05-15T15:13:53+02:00 The Truman Show for protozoan parasites: A review of in vitro cultivation platforms. Smita Sutrave Martin Heinrich Richter 2021-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009668 https://doaj.org/article/bc7477298ff34a628e05a2215f459085 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009668 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009668 https://doaj.org/article/bc7477298ff34a628e05a2215f459085 PLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009668 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009668 2022-12-31T05:20:32Z Protozoan parasites are responsible for severe disease and suffering in humans worldwide. Apart from disease transmission via insect vectors and contaminated soil, food, or water, transmission may occur congenitally or by way of blood transfusion and organ transplantation. Several recent outbreaks associated with fresh produce and potable water emphasize the need for vigilance and monitoring of protozoan parasites that cause severe disease in humans globally. Apart from the tropical parasite Plasmodium spp., other protozoa causing debilitating and fatal diseases such as Trypanosoma spp. and Naegleria fowleri need to be studied in more detail. Climate change and socioeconomic issues such as migration continue to be major drivers for the spread of these neglected tropical diseases beyond endemic zones. Due to the complex life cycles of protozoa involving multiple hosts, vectors, and stringent growth conditions, studying these parasites has been challenging. While in vivo models may provide insights into host-parasite interaction, the ethical aspects of laboratory animal use and the challenge of ready availability of parasite life stages underline the need for in vitro models as valid alternatives for culturing and maintaining protozoan parasites. To our knowledge, this review is the first of its kind to highlight available in vitro models for protozoa causing highly infectious diseases. In recent years, several research efforts using new technologies such as 3D organoid and spheroid systems for protozoan parasites have been introduced that provide valuable tools to advance complex culturing models and offer new opportunities toward the advancement of parasite in vitro studies. In vitro models aid scientists and healthcare providers in gaining insights into parasite infection biology, ultimately enabling the use of novel strategies for preventing and treating these diseases. Article in Journal/Newspaper Arctic Climate change Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 8 e0009668 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Smita Sutrave Martin Heinrich Richter The Truman Show for protozoan parasites: A review of in vitro cultivation platforms. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Protozoan parasites are responsible for severe disease and suffering in humans worldwide. Apart from disease transmission via insect vectors and contaminated soil, food, or water, transmission may occur congenitally or by way of blood transfusion and organ transplantation. Several recent outbreaks associated with fresh produce and potable water emphasize the need for vigilance and monitoring of protozoan parasites that cause severe disease in humans globally. Apart from the tropical parasite Plasmodium spp., other protozoa causing debilitating and fatal diseases such as Trypanosoma spp. and Naegleria fowleri need to be studied in more detail. Climate change and socioeconomic issues such as migration continue to be major drivers for the spread of these neglected tropical diseases beyond endemic zones. Due to the complex life cycles of protozoa involving multiple hosts, vectors, and stringent growth conditions, studying these parasites has been challenging. While in vivo models may provide insights into host-parasite interaction, the ethical aspects of laboratory animal use and the challenge of ready availability of parasite life stages underline the need for in vitro models as valid alternatives for culturing and maintaining protozoan parasites. To our knowledge, this review is the first of its kind to highlight available in vitro models for protozoa causing highly infectious diseases. In recent years, several research efforts using new technologies such as 3D organoid and spheroid systems for protozoan parasites have been introduced that provide valuable tools to advance complex culturing models and offer new opportunities toward the advancement of parasite in vitro studies. In vitro models aid scientists and healthcare providers in gaining insights into parasite infection biology, ultimately enabling the use of novel strategies for preventing and treating these diseases. |
format |
Article in Journal/Newspaper |
author |
Smita Sutrave Martin Heinrich Richter |
author_facet |
Smita Sutrave Martin Heinrich Richter |
author_sort |
Smita Sutrave |
title |
The Truman Show for protozoan parasites: A review of in vitro cultivation platforms. |
title_short |
The Truman Show for protozoan parasites: A review of in vitro cultivation platforms. |
title_full |
The Truman Show for protozoan parasites: A review of in vitro cultivation platforms. |
title_fullStr |
The Truman Show for protozoan parasites: A review of in vitro cultivation platforms. |
title_full_unstemmed |
The Truman Show for protozoan parasites: A review of in vitro cultivation platforms. |
title_sort |
truman show for protozoan parasites: a review of in vitro cultivation platforms. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009668 https://doaj.org/article/bc7477298ff34a628e05a2215f459085 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change |
genre_facet |
Arctic Climate change |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009668 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009668 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009668 https://doaj.org/article/bc7477298ff34a628e05a2215f459085 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009668 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
8 |
container_start_page |
e0009668 |
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1766344396946538496 |