Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood
Abstract Background Plasmodium vivax is the most prevalent human malaria parasite and is likely to increase proportionally as malaria control efforts more rapidly impact the prevalence of Plasmodium falciparum. Despite the prominence of P. vivax as a major human pathogen, vivax malaria qualifies as...
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ftdoajarticles:oai:doaj.org/article:bc68b5637c864aa0b4946ddbf89e7d37 2023-05-15T15:16:25+02:00 Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood Rajeev K. Mehlotra D’Arbra Blankenship Rosalind E. Howes Tovonahary A. Rakotomanga Brune Ramiranirina Stephanie Ramboarina Thierry Franchard Marlin H. Linger Melinda Zikursh-Blood Arsène C. Ratsimbasoa Peter A. Zimmerman Brian T. Grimberg 2017-11-01T00:00:00Z https://doi.org/10.1186/s12936-017-2090-7 https://doaj.org/article/bc68b5637c864aa0b4946ddbf89e7d37 EN eng BMC http://link.springer.com/article/10.1186/s12936-017-2090-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2090-7 1475-2875 https://doaj.org/article/bc68b5637c864aa0b4946ddbf89e7d37 Malaria Journal, Vol 16, Iss 1, Pp 1-13 (2017) Madagascar In vitro culture Plasmodium vivax Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-2090-7 2022-12-31T13:25:43Z Abstract Background Plasmodium vivax is the most prevalent human malaria parasite and is likely to increase proportionally as malaria control efforts more rapidly impact the prevalence of Plasmodium falciparum. Despite the prominence of P. vivax as a major human pathogen, vivax malaria qualifies as a neglected and under-studied tropical disease. Significant challenges bringing P. vivax into the laboratory, particularly the capacity for long-term propagation of well-characterized strains, have limited the study of this parasite’s red blood cell (RBC) invasion mechanism, blood-stage development, gene expression, and genetic manipulation. Methods and results Patient isolates of P. vivax have been collected and cryopreserved in the rural community of Ampasimpotsy, located in the Tsiroanomandidy Health District of Madagascar. Periodic, monthly overland transport of these cryopreserved isolates to the country’s National Malaria Control Programme laboratory in Antananarivo preceded onward sample transfer to laboratories at Case Western Reserve University, USA. There, the P. vivax isolates have been cultured through propagation in the RBCs of Saimiri boliviensis. For the four patient isolates studied to-date, the median time interval between sample collection and in vitro culture has been 454 days (range 166–961 days). The median time in culture, continually documented by light microscopy, has been 159 days; isolate AMP2014.01 was continuously propagated for 233 days. Further studies show that the P. vivax parasites propagated in Saimiri RBCs retain their ability to invade human RBCs, and can be cryopreserved, thawed and successfully returned to productive in vitro culture. Conclusions/significance Long-term culture of P. vivax is possible in the RBCs of Saimiri boliviensis. These studies provide an alternative to propagation of P. vivax in live animals that are becoming more restricted. In vitro culture of P. vivax in Saimiri RBCs provides an opening to stabilize patient isolates, which would serve as precious ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Madagascar In vitro culture Plasmodium vivax Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Madagascar In vitro culture Plasmodium vivax Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Rajeev K. Mehlotra D’Arbra Blankenship Rosalind E. Howes Tovonahary A. Rakotomanga Brune Ramiranirina Stephanie Ramboarina Thierry Franchard Marlin H. Linger Melinda Zikursh-Blood Arsène C. Ratsimbasoa Peter A. Zimmerman Brian T. Grimberg Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood |
topic_facet |
Madagascar In vitro culture Plasmodium vivax Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium vivax is the most prevalent human malaria parasite and is likely to increase proportionally as malaria control efforts more rapidly impact the prevalence of Plasmodium falciparum. Despite the prominence of P. vivax as a major human pathogen, vivax malaria qualifies as a neglected and under-studied tropical disease. Significant challenges bringing P. vivax into the laboratory, particularly the capacity for long-term propagation of well-characterized strains, have limited the study of this parasite’s red blood cell (RBC) invasion mechanism, blood-stage development, gene expression, and genetic manipulation. Methods and results Patient isolates of P. vivax have been collected and cryopreserved in the rural community of Ampasimpotsy, located in the Tsiroanomandidy Health District of Madagascar. Periodic, monthly overland transport of these cryopreserved isolates to the country’s National Malaria Control Programme laboratory in Antananarivo preceded onward sample transfer to laboratories at Case Western Reserve University, USA. There, the P. vivax isolates have been cultured through propagation in the RBCs of Saimiri boliviensis. For the four patient isolates studied to-date, the median time interval between sample collection and in vitro culture has been 454 days (range 166–961 days). The median time in culture, continually documented by light microscopy, has been 159 days; isolate AMP2014.01 was continuously propagated for 233 days. Further studies show that the P. vivax parasites propagated in Saimiri RBCs retain their ability to invade human RBCs, and can be cryopreserved, thawed and successfully returned to productive in vitro culture. Conclusions/significance Long-term culture of P. vivax is possible in the RBCs of Saimiri boliviensis. These studies provide an alternative to propagation of P. vivax in live animals that are becoming more restricted. In vitro culture of P. vivax in Saimiri RBCs provides an opening to stabilize patient isolates, which would serve as precious ... |
format |
Article in Journal/Newspaper |
author |
Rajeev K. Mehlotra D’Arbra Blankenship Rosalind E. Howes Tovonahary A. Rakotomanga Brune Ramiranirina Stephanie Ramboarina Thierry Franchard Marlin H. Linger Melinda Zikursh-Blood Arsène C. Ratsimbasoa Peter A. Zimmerman Brian T. Grimberg |
author_facet |
Rajeev K. Mehlotra D’Arbra Blankenship Rosalind E. Howes Tovonahary A. Rakotomanga Brune Ramiranirina Stephanie Ramboarina Thierry Franchard Marlin H. Linger Melinda Zikursh-Blood Arsène C. Ratsimbasoa Peter A. Zimmerman Brian T. Grimberg |
author_sort |
Rajeev K. Mehlotra |
title |
Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood |
title_short |
Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood |
title_full |
Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood |
title_fullStr |
Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood |
title_full_unstemmed |
Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood |
title_sort |
long-term in vitro culture of plasmodium vivax isolates from madagascar maintained in saimiri boliviensis blood |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-2090-7 https://doaj.org/article/bc68b5637c864aa0b4946ddbf89e7d37 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-13 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-2090-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2090-7 1475-2875 https://doaj.org/article/bc68b5637c864aa0b4946ddbf89e7d37 |
op_doi |
https://doi.org/10.1186/s12936-017-2090-7 |
container_title |
Malaria Journal |
container_volume |
16 |
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1 |
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1766346701228998656 |