Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control
There is a growing acceptance worldwide for the application of dispersants as a marine oil spill response strategy. The development of more effective dispersants with less toxicity and higher biodegradability would be a step forward in improving public acceptance and regulatory approvals for their u...
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ftdoajarticles:oai:doaj.org/article:bc33af819f8a4db583e560e40d75a283 2023-05-15T13:09:11+02:00 Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control Zhiwen Zhu Baiyu Zhang Qinhong Cai Jingjing Ling Kenneth Lee Bing Chen 2020-07-01T00:00:00Z https://doi.org/10.3389/fbioe.2020.00734 https://doaj.org/article/bc33af819f8a4db583e560e40d75a283 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fbioe.2020.00734/full https://doaj.org/toc/2296-4185 2296-4185 doi:10.3389/fbioe.2020.00734 https://doaj.org/article/bc33af819f8a4db583e560e40d75a283 Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) lipopeptide biosurfactant bio-dispersant biotechnology oil spill response waste management TP248.13-248.65 article 2020 ftdoajarticles https://doi.org/10.3389/fbioe.2020.00734 2022-12-31T14:22:27Z There is a growing acceptance worldwide for the application of dispersants as a marine oil spill response strategy. The development of more effective dispersants with less toxicity and higher biodegradability would be a step forward in improving public acceptance and regulatory approvals for their use. By applying advances in environmental biotechnology, a bio-dispersant agent with a lipopeptide biosurfactant produced by Bacillus subtilis N3-1P as the key component was formulated in this study. The economic feasibility of producing biosurfactant (a high-added-value bioproduct) from fish waste-based peptone as a nutrient substrate was evaluated. Protein hydrolyzate was prepared from cod liver and head wastes obtained from fish processing facilities. Hydrolysis conditions (i.e., time, temperature, pH and enzyme to substrate level) for preparing protein hydrolyzates were optimized by response surface methodology using a factorial design. The critical micelle dilution (CMD) value for biosurfactant produced from the fish liver and head waste generated peptones was 54.72 and 47.59 CMD, respectively. Biosurfactant product generated by fish liver peptone had a low critical micelle concentration of 0.18 g L–1 and could reduce the surface tension of distilled water to 27.9 mN/m. Structure characterization proved that the generated biosurfactant product belongs to the lipopeptide class. An alternative to the key surfactant dioctyl sulfosuccinate sodium (DOSS) used in Corexit 9500 has been proposed based on a binary mixture of lipopeptides and DOSS that exhibited synergistic effects. Using the standard baffled flask test, a high dispersion efficiency of 76.8% for Alaska North Slope oil was achieved at a biodispersant composition of 80/20 (v/v) of lipopeptides/DOSS. The results show that fish waste can be utilized to produce a more effective, environmentally acceptable and cost-efficient biodispersant that can be applied to oil spills in the marine environment. Article in Journal/Newspaper Alaska North Slope north slope Alaska Directory of Open Access Journals: DOAJ Articles Frontiers in Bioengineering and Biotechnology 8 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
lipopeptide biosurfactant bio-dispersant biotechnology oil spill response waste management TP248.13-248.65 |
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lipopeptide biosurfactant bio-dispersant biotechnology oil spill response waste management TP248.13-248.65 Zhiwen Zhu Baiyu Zhang Qinhong Cai Jingjing Ling Kenneth Lee Bing Chen Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control |
topic_facet |
lipopeptide biosurfactant bio-dispersant biotechnology oil spill response waste management TP248.13-248.65 |
description |
There is a growing acceptance worldwide for the application of dispersants as a marine oil spill response strategy. The development of more effective dispersants with less toxicity and higher biodegradability would be a step forward in improving public acceptance and regulatory approvals for their use. By applying advances in environmental biotechnology, a bio-dispersant agent with a lipopeptide biosurfactant produced by Bacillus subtilis N3-1P as the key component was formulated in this study. The economic feasibility of producing biosurfactant (a high-added-value bioproduct) from fish waste-based peptone as a nutrient substrate was evaluated. Protein hydrolyzate was prepared from cod liver and head wastes obtained from fish processing facilities. Hydrolysis conditions (i.e., time, temperature, pH and enzyme to substrate level) for preparing protein hydrolyzates were optimized by response surface methodology using a factorial design. The critical micelle dilution (CMD) value for biosurfactant produced from the fish liver and head waste generated peptones was 54.72 and 47.59 CMD, respectively. Biosurfactant product generated by fish liver peptone had a low critical micelle concentration of 0.18 g L–1 and could reduce the surface tension of distilled water to 27.9 mN/m. Structure characterization proved that the generated biosurfactant product belongs to the lipopeptide class. An alternative to the key surfactant dioctyl sulfosuccinate sodium (DOSS) used in Corexit 9500 has been proposed based on a binary mixture of lipopeptides and DOSS that exhibited synergistic effects. Using the standard baffled flask test, a high dispersion efficiency of 76.8% for Alaska North Slope oil was achieved at a biodispersant composition of 80/20 (v/v) of lipopeptides/DOSS. The results show that fish waste can be utilized to produce a more effective, environmentally acceptable and cost-efficient biodispersant that can be applied to oil spills in the marine environment. |
format |
Article in Journal/Newspaper |
author |
Zhiwen Zhu Baiyu Zhang Qinhong Cai Jingjing Ling Kenneth Lee Bing Chen |
author_facet |
Zhiwen Zhu Baiyu Zhang Qinhong Cai Jingjing Ling Kenneth Lee Bing Chen |
author_sort |
Zhiwen Zhu |
title |
Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control |
title_short |
Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control |
title_full |
Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control |
title_fullStr |
Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control |
title_full_unstemmed |
Fish Waste Based Lipopeptide Production and the Potential Application as a Bio-Dispersant for Oil Spill Control |
title_sort |
fish waste based lipopeptide production and the potential application as a bio-dispersant for oil spill control |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fbioe.2020.00734 https://doaj.org/article/bc33af819f8a4db583e560e40d75a283 |
genre |
Alaska North Slope north slope Alaska |
genre_facet |
Alaska North Slope north slope Alaska |
op_source |
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fbioe.2020.00734/full https://doaj.org/toc/2296-4185 2296-4185 doi:10.3389/fbioe.2020.00734 https://doaj.org/article/bc33af819f8a4db583e560e40d75a283 |
op_doi |
https://doi.org/10.3389/fbioe.2020.00734 |
container_title |
Frontiers in Bioengineering and Biotechnology |
container_volume |
8 |
_version_ |
1766166324910751744 |