Effect of polyoxyethylene sorbitan monooleate (Tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast
Studies on the surface-active agents added during the cultivation process proved their effect on the synthesized bioproducts. The biosynthesis and accumulation of exopolysaccharide and biomass by Antarctic producer Cryptococcus laurentii AL65 were studied in submerged cultivation using different car...
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ftdoajarticles:oai:doaj.org/article:bc2a5991b4e340cba22913069c0c24b7 2023-12-10T09:43:02+01:00 Effect of polyoxyethylene sorbitan monooleate (Tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast Snezhana Rusinova-Videva Zhana Petkova Ginka Antova Plamen Stoyanov Tsvetelina Mladenova Tzenka Radoukova 2023-07-01T00:00:00Z https://doi.org/10.24425/ppr.2023.145437 https://doaj.org/article/bc2a5991b4e340cba22913069c0c24b7 EN eng Polish Academy of Sciences https://journals.pan.pl/Content/127845/PDF/Accepted_Rusinowa.pdf https://doaj.org/toc/0138-0338 https://doaj.org/toc/2081-8262 0138-0338 2081-8262 https://doi.org/10.24425/ppr.2023.145437 https://doaj.org/article/bc2a5991b4e340cba22913069c0c24b7 Polish Polar Research, Vol vol. 44, Iss No 4, Pp 385-399 (2023) antarctic cryptococcus laurentii biomass lipids Geology QE1-996.5 article 2023 ftdoajarticles https://doi.org/10.24425/ppr.2023.145437 2023-11-12T01:39:09Z Studies on the surface-active agents added during the cultivation process proved their effect on the synthesized bioproducts. The biosynthesis and accumulation of exopolysaccharide and biomass by Antarctic producer Cryptococcus laurentii AL65 were studied in submerged cultivation using different carbon sources in the culture medium added to a one-percent concentration (pentoses, hexoses and oligosaccharides). Sucrose was chosen as the most appropriate carbon source for the cell growth and extracellular polymer biosynthesis. Absorption of sucrose by a strain producer was monitored and the residual sugars were traced in the dynamics of the fermentation process. The effect of polyoxyethylene sorbitan monooleate (Tween 80) on the cell growth and the accumulation of the synthesized biopolymer were studied for the first time with the Antarctic producer. The optimal increase in the amount of accumulated exopolysaccharide was recorded at a concentration of 6% Tween 80 at 24 h from the start of fermentation. The surfactant treatment contributed to an increase in the total amount of lipids in the cell, to the increased biosynthesis of unsaturated fatty acids compared to saturated ones, and to the increased cellular synthesis of valuable metabolites such as phosphatidylinositol, phosphatidylserine and phosphatidylethanolamine. The impact of Tween 80 on intracellular and extracellular metabolites is a valuable asset in a possible scale-up of the process in industrial settings. Article in Journal/Newspaper Antarc* Antarctic Polar Research Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Polish Polar Research |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
antarctic cryptococcus laurentii biomass lipids Geology QE1-996.5 |
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antarctic cryptococcus laurentii biomass lipids Geology QE1-996.5 Snezhana Rusinova-Videva Zhana Petkova Ginka Antova Plamen Stoyanov Tsvetelina Mladenova Tzenka Radoukova Effect of polyoxyethylene sorbitan monooleate (Tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast |
topic_facet |
antarctic cryptococcus laurentii biomass lipids Geology QE1-996.5 |
description |
Studies on the surface-active agents added during the cultivation process proved their effect on the synthesized bioproducts. The biosynthesis and accumulation of exopolysaccharide and biomass by Antarctic producer Cryptococcus laurentii AL65 were studied in submerged cultivation using different carbon sources in the culture medium added to a one-percent concentration (pentoses, hexoses and oligosaccharides). Sucrose was chosen as the most appropriate carbon source for the cell growth and extracellular polymer biosynthesis. Absorption of sucrose by a strain producer was monitored and the residual sugars were traced in the dynamics of the fermentation process. The effect of polyoxyethylene sorbitan monooleate (Tween 80) on the cell growth and the accumulation of the synthesized biopolymer were studied for the first time with the Antarctic producer. The optimal increase in the amount of accumulated exopolysaccharide was recorded at a concentration of 6% Tween 80 at 24 h from the start of fermentation. The surfactant treatment contributed to an increase in the total amount of lipids in the cell, to the increased biosynthesis of unsaturated fatty acids compared to saturated ones, and to the increased cellular synthesis of valuable metabolites such as phosphatidylinositol, phosphatidylserine and phosphatidylethanolamine. The impact of Tween 80 on intracellular and extracellular metabolites is a valuable asset in a possible scale-up of the process in industrial settings. |
format |
Article in Journal/Newspaper |
author |
Snezhana Rusinova-Videva Zhana Petkova Ginka Antova Plamen Stoyanov Tsvetelina Mladenova Tzenka Radoukova |
author_facet |
Snezhana Rusinova-Videva Zhana Petkova Ginka Antova Plamen Stoyanov Tsvetelina Mladenova Tzenka Radoukova |
author_sort |
Snezhana Rusinova-Videva |
title |
Effect of polyoxyethylene sorbitan monooleate (Tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast |
title_short |
Effect of polyoxyethylene sorbitan monooleate (Tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast |
title_full |
Effect of polyoxyethylene sorbitan monooleate (Tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast |
title_fullStr |
Effect of polyoxyethylene sorbitan monooleate (Tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast |
title_full_unstemmed |
Effect of polyoxyethylene sorbitan monooleate (Tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast |
title_sort |
effect of polyoxyethylene sorbitan monooleate (tween 80) on exopolysaccharide and biomass composition of psycchrophilic yeast |
publisher |
Polish Academy of Sciences |
publishDate |
2023 |
url |
https://doi.org/10.24425/ppr.2023.145437 https://doaj.org/article/bc2a5991b4e340cba22913069c0c24b7 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Polar Research |
genre_facet |
Antarc* Antarctic Polar Research |
op_source |
Polish Polar Research, Vol vol. 44, Iss No 4, Pp 385-399 (2023) |
op_relation |
https://journals.pan.pl/Content/127845/PDF/Accepted_Rusinowa.pdf https://doaj.org/toc/0138-0338 https://doaj.org/toc/2081-8262 0138-0338 2081-8262 https://doi.org/10.24425/ppr.2023.145437 https://doaj.org/article/bc2a5991b4e340cba22913069c0c24b7 |
op_doi |
https://doi.org/10.24425/ppr.2023.145437 |
container_title |
Polish Polar Research |
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1784886153314304000 |