Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion.
The interaction between Plasmodium vivax Duffy binding protein (PvDBP) and Duffy antigen receptor for chemokines (DARC) has been described as critical for the invasion of human reticulocytes, although increasing reports of P. vivax infections in Duffy-negative individuals questions its unique role....
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ftdoajarticles:oai:doaj.org/article:bc133b6ffc604df396cf0908b8880fac 2023-05-15T15:08:31+02:00 Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion. Camille Roesch Jean Popovici Sophalai Bin Vorleak Run Saorin Kim Stéphanie Ramboarina Emma Rakotomalala Rado Lalaina Rakotoarison Tsikiniaina Rasoloharimanana Zo Andriamanantena Anuj Kumar Micheline Guillotte-Blisnick Christèle Huon David Serre Chetan E Chitnis Inès Vigan-Womas Didier Menard 2018-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006555 https://doaj.org/article/bc133b6ffc604df396cf0908b8880fac EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6211765?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006555 https://doaj.org/article/bc133b6ffc604df396cf0908b8880fac PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006555 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006555 2022-12-31T01:49:19Z The interaction between Plasmodium vivax Duffy binding protein (PvDBP) and Duffy antigen receptor for chemokines (DARC) has been described as critical for the invasion of human reticulocytes, although increasing reports of P. vivax infections in Duffy-negative individuals questions its unique role. To investigate the genetic diversity of the two main protein ligands for reticulocyte invasion, PvDBP and P. vivax Erythrocyte Binding Protein (PvEBP), we analyzed 458 isolates collected in Cambodia and Madagascar from individuals genotyped as Duffy-positive. First, we observed a high proportion of isolates with multiple copies PvEBP from Madagascar (56%) where Duffy negative and positive individuals coexist compared to Cambodia (19%) where Duffy-negative population is virtually absent. Whether the gene amplification observed is responsible for alternate invasion pathways remains to be tested. Second, we found that the PvEBP gene was less diverse than PvDBP gene (12 vs. 33 alleles) but provided evidence for an excess of nonsynonymous mutations with the complete absence of synonymous mutations. This finding reveals that PvEBP is under strong diversifying selection, and confirms the importance of this protein ligand in the invasion process of the human reticulocytes and as a target of acquired immunity. These observations highlight how genomic changes in parasite ligands improve the fitness of P. vivax isolates in the face of immune pressure and receptor polymorphisms. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 10 e0006555 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Camille Roesch Jean Popovici Sophalai Bin Vorleak Run Saorin Kim Stéphanie Ramboarina Emma Rakotomalala Rado Lalaina Rakotoarison Tsikiniaina Rasoloharimanana Zo Andriamanantena Anuj Kumar Micheline Guillotte-Blisnick Christèle Huon David Serre Chetan E Chitnis Inès Vigan-Womas Didier Menard Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The interaction between Plasmodium vivax Duffy binding protein (PvDBP) and Duffy antigen receptor for chemokines (DARC) has been described as critical for the invasion of human reticulocytes, although increasing reports of P. vivax infections in Duffy-negative individuals questions its unique role. To investigate the genetic diversity of the two main protein ligands for reticulocyte invasion, PvDBP and P. vivax Erythrocyte Binding Protein (PvEBP), we analyzed 458 isolates collected in Cambodia and Madagascar from individuals genotyped as Duffy-positive. First, we observed a high proportion of isolates with multiple copies PvEBP from Madagascar (56%) where Duffy negative and positive individuals coexist compared to Cambodia (19%) where Duffy-negative population is virtually absent. Whether the gene amplification observed is responsible for alternate invasion pathways remains to be tested. Second, we found that the PvEBP gene was less diverse than PvDBP gene (12 vs. 33 alleles) but provided evidence for an excess of nonsynonymous mutations with the complete absence of synonymous mutations. This finding reveals that PvEBP is under strong diversifying selection, and confirms the importance of this protein ligand in the invasion process of the human reticulocytes and as a target of acquired immunity. These observations highlight how genomic changes in parasite ligands improve the fitness of P. vivax isolates in the face of immune pressure and receptor polymorphisms. |
format |
Article in Journal/Newspaper |
author |
Camille Roesch Jean Popovici Sophalai Bin Vorleak Run Saorin Kim Stéphanie Ramboarina Emma Rakotomalala Rado Lalaina Rakotoarison Tsikiniaina Rasoloharimanana Zo Andriamanantena Anuj Kumar Micheline Guillotte-Blisnick Christèle Huon David Serre Chetan E Chitnis Inès Vigan-Womas Didier Menard |
author_facet |
Camille Roesch Jean Popovici Sophalai Bin Vorleak Run Saorin Kim Stéphanie Ramboarina Emma Rakotomalala Rado Lalaina Rakotoarison Tsikiniaina Rasoloharimanana Zo Andriamanantena Anuj Kumar Micheline Guillotte-Blisnick Christèle Huon David Serre Chetan E Chitnis Inès Vigan-Womas Didier Menard |
author_sort |
Camille Roesch |
title |
Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion. |
title_short |
Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion. |
title_full |
Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion. |
title_fullStr |
Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion. |
title_full_unstemmed |
Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion. |
title_sort |
genetic diversity in two plasmodium vivax protein ligands for reticulocyte invasion. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006555 https://doaj.org/article/bc133b6ffc604df396cf0908b8880fac |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006555 (2018) |
op_relation |
http://europepmc.org/articles/PMC6211765?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006555 https://doaj.org/article/bc133b6ffc604df396cf0908b8880fac |
op_doi |
https://doi.org/10.1371/journal.pntd.0006555 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
10 |
container_start_page |
e0006555 |
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1766339874169815040 |