Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice
Objective: To determine the involvement and the modulatory effects of IL-33 during Plasmodium berghei ANKA (PbA) infection. Methods: PbA infection in male ICR mice was utilized as a model of malaria. Systemically circulating IL-33 levels were determined in blood plasma by enzyme-linked immunosorbent...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2023
|
Subjects: | |
Online Access: | https://doi.org/10.4103/2221-1691.391157 https://doaj.org/article/bbcd485698064710a11c2f48daa11765 |
id |
ftdoajarticles:oai:doaj.org/article:bbcd485698064710a11c2f48daa11765 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:bbcd485698064710a11c2f48daa11765 2024-09-09T19:26:08+00:00 Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice Mohammad Faruq Abd Rachman Isnadi Rusliza Basir Ramatu Bello Omenesa Majid Roslaini Abd Abdullah Maizaton Atmadini Taib Che Norma Mat Priya Sivan Padma Yong Yean Kong Chin Voon Kin Gambo Lawal Mukhtar 2023-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.391157 https://doaj.org/article/bbcd485698064710a11c2f48daa11765 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=12;spage=521;epage=531;aulast=Rachman https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.391157 https://doaj.org/article/bbcd485698064710a11c2f48daa11765 Asian Pacific Journal of Tropical Biomedicine, Vol 13, Iss 12, Pp 521-531 (2023) plasmodium berghei anka malaria il-33 immunology immunotherapy Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.4103/2221-1691.391157 2024-08-05T17:49:19Z Objective: To determine the involvement and the modulatory effects of IL-33 during Plasmodium berghei ANKA (PbA) infection. Methods: PbA infection in male ICR mice was utilized as a model of malaria. Systemically circulating IL-33 levels were determined in blood plasma by enzyme-linked immunosorbent assay (ELISA). After 24 hours post-inoculation of PbA, recombinant IL-33 and ST2, and antibodies against IL-33 and IgG treatments were administered daily for 3 days. Tissue expression and localization of IL-33 were assessed in organs generally affected by malaria via immunohistochemistry. Moreover, histopathological examination was performed to assess the effects of the treatments. Results: The levels of systemic IL-33 were elevated at the critical phase of PbA infection. Likewise, immunohistochemical analysis revealed a significant upregulation of IL-33 expression at the critical phase in the brain, lungs, and spleen of PbA-infected mice as compared to healthy controls. Treatment with IL-33 protected against experimental cerebral malaria development and reduced pathological features in the brain and lungs of the PbA-infected mice. Conclusions: A potential critical role and involvement of IL-33 in PbA infection may hint at the resolution of immunopathological sequelae associated with malaria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 13 12 521 231 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
plasmodium berghei anka malaria il-33 immunology immunotherapy Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
plasmodium berghei anka malaria il-33 immunology immunotherapy Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Mohammad Faruq Abd Rachman Isnadi Rusliza Basir Ramatu Bello Omenesa Majid Roslaini Abd Abdullah Maizaton Atmadini Taib Che Norma Mat Priya Sivan Padma Yong Yean Kong Chin Voon Kin Gambo Lawal Mukhtar Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
topic_facet |
plasmodium berghei anka malaria il-33 immunology immunotherapy Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the involvement and the modulatory effects of IL-33 during Plasmodium berghei ANKA (PbA) infection. Methods: PbA infection in male ICR mice was utilized as a model of malaria. Systemically circulating IL-33 levels were determined in blood plasma by enzyme-linked immunosorbent assay (ELISA). After 24 hours post-inoculation of PbA, recombinant IL-33 and ST2, and antibodies against IL-33 and IgG treatments were administered daily for 3 days. Tissue expression and localization of IL-33 were assessed in organs generally affected by malaria via immunohistochemistry. Moreover, histopathological examination was performed to assess the effects of the treatments. Results: The levels of systemic IL-33 were elevated at the critical phase of PbA infection. Likewise, immunohistochemical analysis revealed a significant upregulation of IL-33 expression at the critical phase in the brain, lungs, and spleen of PbA-infected mice as compared to healthy controls. Treatment with IL-33 protected against experimental cerebral malaria development and reduced pathological features in the brain and lungs of the PbA-infected mice. Conclusions: A potential critical role and involvement of IL-33 in PbA infection may hint at the resolution of immunopathological sequelae associated with malaria. |
format |
Article in Journal/Newspaper |
author |
Mohammad Faruq Abd Rachman Isnadi Rusliza Basir Ramatu Bello Omenesa Majid Roslaini Abd Abdullah Maizaton Atmadini Taib Che Norma Mat Priya Sivan Padma Yong Yean Kong Chin Voon Kin Gambo Lawal Mukhtar |
author_facet |
Mohammad Faruq Abd Rachman Isnadi Rusliza Basir Ramatu Bello Omenesa Majid Roslaini Abd Abdullah Maizaton Atmadini Taib Che Norma Mat Priya Sivan Padma Yong Yean Kong Chin Voon Kin Gambo Lawal Mukhtar |
author_sort |
Mohammad Faruq Abd Rachman Isnadi |
title |
Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
title_short |
Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
title_full |
Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
title_fullStr |
Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
title_full_unstemmed |
Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
title_sort |
interleukin-33 exerts pleiotropic immunoregulatory effects in response to plasmodium berghei anka (pba) infection in mice |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2023 |
url |
https://doi.org/10.4103/2221-1691.391157 https://doaj.org/article/bbcd485698064710a11c2f48daa11765 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 13, Iss 12, Pp 521-531 (2023) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=12;spage=521;epage=531;aulast=Rachman https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.391157 https://doaj.org/article/bbcd485698064710a11c2f48daa11765 |
op_doi |
https://doi.org/10.4103/2221-1691.391157 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
13 |
container_issue |
12 |
container_start_page |
521 |
op_container_end_page |
231 |
_version_ |
1809895801582780416 |