Identification of phytochemical compounds in Calophyllum inophyllum leaves

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: David Febrilliant Susanto, Hakun Wirawasista Aparamarta, Arief Widjaja, Setiyo Gunawan
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: Calophyllum inophyllum leaves; Crude extracts; Proximate composition; Mineral composition; Phytochemical; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2017.08.001; https://doaj.org/article/bbb2241acfd946beac6426fbaab6f445

Objectives: To investigate the proximate composition, mineral content, and phytochemical compounds in Calophyllum inophyllum (C. inophyllum) leaves. Moreover, isolation and identification of pyrene were also performed. Methods: C. inophyllum leaves were extracted with methanol by percolation methods. The proximate composition of C. inophyllum leaves was analyzed by standard methods. Mineral contents in this plant were analyzed by using atomic absorption spectrophotometer. Phytochemical screening and analysis of this plant were performed by spectrophotometric method. Washing method with carbon disulfide was used for isolating dihydropyrene compound from C. inophyllum leaves extracts. Results: The result revealed that C. inophyllum leaves contained 11.24% moisture, 4.75% ash, 6.43% crude protein, 23.96% crude fiber, 9.91% carbohydrate, and energy (79.17 kcal/100 g). The leaves also contained 0.007% iron, 1.240% calcium, 0.075% sodium, 0.195% magnesium, 0.100% ppm potassium, and 0.040% phosphorus. Moreover, 11.51% alkaloid, 2.48% triterpenoid, 2.37% flavonoid, 7.68% tannin, 2.16% saponin, 2.53% polyphenol, were identified in the methanolic crude extracts of C. inophyllum leaves. It was found that trans-2-[2-(trifluoromethyl)phenyl]-10b,10c-dimethyl-10b,10c-dihydropyrene was obtained at purity of 79.18% (22.17% yield) from C. inophyllum leaves. Conclusions: C. inophyllum leaves may be used as a good source of fiber. It was found that C. inophyllum leaves have the potential as herbal drugs due to their phytochemical content. The separation, isolation, and purification of bioactive compounds from this methanolic crude extract and their biological activity are under further investigation.