Hypoxia adaptations in the grey wolf (Canis lupus chanco) from Qinghai-Tibet Plateau.
The Tibetan grey wolf (Canis lupus chanco) occupies habitats on the Qinghai-Tibet Plateau, a high altitude (>3000 m) environment where low oxygen tension exerts unique selection pressure on individuals to adapt to hypoxic conditions. To identify genes involved in hypoxia adaptation, we generated...
Published in: | PLoS Genetics |
---|---|
Main Authors: | , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pgen.1004466 https://doaj.org/article/bbb06cf26cd446c7a33a18c6ecc963fb |
id |
ftdoajarticles:oai:doaj.org/article:bbb06cf26cd446c7a33a18c6ecc963fb |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:bbb06cf26cd446c7a33a18c6ecc963fb 2023-05-15T15:49:53+02:00 Hypoxia adaptations in the grey wolf (Canis lupus chanco) from Qinghai-Tibet Plateau. Wenping Zhang Zhenxin Fan Eunjung Han Rong Hou Liang Zhang Marco Galaverni Jie Huang Hong Liu Pedro Silva Peng Li John P Pollinger Lianming Du XiuyYue Zhang Bisong Yue Robert K Wayne Zhihe Zhang 2014-07-01T00:00:00Z https://doi.org/10.1371/journal.pgen.1004466 https://doaj.org/article/bbb06cf26cd446c7a33a18c6ecc963fb EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4117439?pdf=render https://doaj.org/toc/1553-7390 https://doaj.org/toc/1553-7404 1553-7390 1553-7404 doi:10.1371/journal.pgen.1004466 https://doaj.org/article/bbb06cf26cd446c7a33a18c6ecc963fb PLoS Genetics, Vol 10, Iss 7, p e1004466 (2014) Genetics QH426-470 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pgen.1004466 2022-12-30T22:10:48Z The Tibetan grey wolf (Canis lupus chanco) occupies habitats on the Qinghai-Tibet Plateau, a high altitude (>3000 m) environment where low oxygen tension exerts unique selection pressure on individuals to adapt to hypoxic conditions. To identify genes involved in hypoxia adaptation, we generated complete genome sequences of nine Chinese wolves from high and low altitude populations at an average coverage of 25× coverage. We found that, beginning about 55,000 years ago, the highland Tibetan grey wolf suffered a more substantial population decline than lowland wolves. Positively selected hypoxia-related genes in highland wolves are enriched in the HIF signaling pathway (P = 1.57E-6), ATP binding (P = 5.62E-5), and response to an oxygen-containing compound (P≤5.30E-4). Of these positively selected hypoxia-related genes, three genes (EPAS1, ANGPT1, and RYR2) had at least one specific fixed non-synonymous SNP in highland wolves based on the nine genome data. Our re-sequencing studies on a large panel of individuals showed a frequency difference greater than 58% between highland and lowland wolves for these specific fixed non-synonymous SNPs and a high degree of LD surrounding the three genes, which imply strong selection. Past studies have shown that EPAS1 and ANGPT1 are important in the response to hypoxic stress, and RYR2 is involved in heart function. These three genes also exhibited significant signals of natural selection in high altitude human populations, which suggest similar evolutionary constraints on natural selection in wolves and humans of the Qinghai-Tibet Plateau. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles PLoS Genetics 10 7 e1004466 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Genetics QH426-470 |
spellingShingle |
Genetics QH426-470 Wenping Zhang Zhenxin Fan Eunjung Han Rong Hou Liang Zhang Marco Galaverni Jie Huang Hong Liu Pedro Silva Peng Li John P Pollinger Lianming Du XiuyYue Zhang Bisong Yue Robert K Wayne Zhihe Zhang Hypoxia adaptations in the grey wolf (Canis lupus chanco) from Qinghai-Tibet Plateau. |
topic_facet |
Genetics QH426-470 |
description |
The Tibetan grey wolf (Canis lupus chanco) occupies habitats on the Qinghai-Tibet Plateau, a high altitude (>3000 m) environment where low oxygen tension exerts unique selection pressure on individuals to adapt to hypoxic conditions. To identify genes involved in hypoxia adaptation, we generated complete genome sequences of nine Chinese wolves from high and low altitude populations at an average coverage of 25× coverage. We found that, beginning about 55,000 years ago, the highland Tibetan grey wolf suffered a more substantial population decline than lowland wolves. Positively selected hypoxia-related genes in highland wolves are enriched in the HIF signaling pathway (P = 1.57E-6), ATP binding (P = 5.62E-5), and response to an oxygen-containing compound (P≤5.30E-4). Of these positively selected hypoxia-related genes, three genes (EPAS1, ANGPT1, and RYR2) had at least one specific fixed non-synonymous SNP in highland wolves based on the nine genome data. Our re-sequencing studies on a large panel of individuals showed a frequency difference greater than 58% between highland and lowland wolves for these specific fixed non-synonymous SNPs and a high degree of LD surrounding the three genes, which imply strong selection. Past studies have shown that EPAS1 and ANGPT1 are important in the response to hypoxic stress, and RYR2 is involved in heart function. These three genes also exhibited significant signals of natural selection in high altitude human populations, which suggest similar evolutionary constraints on natural selection in wolves and humans of the Qinghai-Tibet Plateau. |
format |
Article in Journal/Newspaper |
author |
Wenping Zhang Zhenxin Fan Eunjung Han Rong Hou Liang Zhang Marco Galaverni Jie Huang Hong Liu Pedro Silva Peng Li John P Pollinger Lianming Du XiuyYue Zhang Bisong Yue Robert K Wayne Zhihe Zhang |
author_facet |
Wenping Zhang Zhenxin Fan Eunjung Han Rong Hou Liang Zhang Marco Galaverni Jie Huang Hong Liu Pedro Silva Peng Li John P Pollinger Lianming Du XiuyYue Zhang Bisong Yue Robert K Wayne Zhihe Zhang |
author_sort |
Wenping Zhang |
title |
Hypoxia adaptations in the grey wolf (Canis lupus chanco) from Qinghai-Tibet Plateau. |
title_short |
Hypoxia adaptations in the grey wolf (Canis lupus chanco) from Qinghai-Tibet Plateau. |
title_full |
Hypoxia adaptations in the grey wolf (Canis lupus chanco) from Qinghai-Tibet Plateau. |
title_fullStr |
Hypoxia adaptations in the grey wolf (Canis lupus chanco) from Qinghai-Tibet Plateau. |
title_full_unstemmed |
Hypoxia adaptations in the grey wolf (Canis lupus chanco) from Qinghai-Tibet Plateau. |
title_sort |
hypoxia adaptations in the grey wolf (canis lupus chanco) from qinghai-tibet plateau. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pgen.1004466 https://doaj.org/article/bbb06cf26cd446c7a33a18c6ecc963fb |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
PLoS Genetics, Vol 10, Iss 7, p e1004466 (2014) |
op_relation |
http://europepmc.org/articles/PMC4117439?pdf=render https://doaj.org/toc/1553-7390 https://doaj.org/toc/1553-7404 1553-7390 1553-7404 doi:10.1371/journal.pgen.1004466 https://doaj.org/article/bbb06cf26cd446c7a33a18c6ecc963fb |
op_doi |
https://doi.org/10.1371/journal.pgen.1004466 |
container_title |
PLoS Genetics |
container_volume |
10 |
container_issue |
7 |
container_start_page |
e1004466 |
_version_ |
1766384902228410368 |