Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress
The aim of this study was to purify and identify antioxidant peptides from Antarctic Krill hydrolysate and elucidate their cytoprotection mechanisms on H2O2-induced oxidative stress in Chang liver cells. Using ultrafiltration and chromatographic methods, twelve peptides were purified from the Antarc...
Published in: | Journal of Functional Foods |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2021
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.jff.2021.104701 https://doaj.org/article/bb4dbd998d124a98b74857f2703dc812 |
id |
ftdoajarticles:oai:doaj.org/article:bb4dbd998d124a98b74857f2703dc812 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:bb4dbd998d124a98b74857f2703dc812 2023-05-15T14:02:51+02:00 Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress Yue-Zhen Wang Yu-Qin Zhao Yu-Mei Wang Wen-Hao Zhao Peng Wang Chang-Feng Chi Bin Wang 2021-11-01T00:00:00Z https://doi.org/10.1016/j.jff.2021.104701 https://doaj.org/article/bb4dbd998d124a98b74857f2703dc812 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S1756464621003509 https://doaj.org/toc/1756-4646 1756-4646 doi:10.1016/j.jff.2021.104701 https://doaj.org/article/bb4dbd998d124a98b74857f2703dc812 Journal of Functional Foods, Vol 86, Iss , Pp 104701- (2021) Antarctic Krill (Euphausia superba) Antioxidant peptide Radical scavenging activity Oxidative stress Cytoprotection Nutrition. Foods and food supply TX341-641 article 2021 ftdoajarticles https://doi.org/10.1016/j.jff.2021.104701 2022-12-31T06:34:14Z The aim of this study was to purify and identify antioxidant peptides from Antarctic Krill hydrolysate and elucidate their cytoprotection mechanisms on H2O2-induced oxidative stress in Chang liver cells. Using ultrafiltration and chromatographic methods, twelve peptides were purified from the Antarctic Krill hydrolysate and identified as AEK, VEK, VEKT, AEKTR, IEN, VEKGK, LKPGN, IEKG, LQP, ATH, IEKT, and IDSQ. LKPGN and LQP exhibited high scavenging activities on HO·, DPPH·, and O2−·. Moreover, LKPGN and LQP can protect Chang liver cells against H2O2-induced oxidative stress by increasing cell viability and decreasing the apoptosis rate. The mechanism indicated that LKPGN and LQP could concentration-dependently increase the antioxidant enzyme (SOD and GSH-PX) levels to scavenge excess ROS, increase mitochondrial membrane potential, and decrease DNA damage and MDA content. Therefore, LKPGN and LQP from Antarctic Krill could serve as promising candidates applied in health-promoting products. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Journal of Functional Foods 86 104701 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic Krill (Euphausia superba) Antioxidant peptide Radical scavenging activity Oxidative stress Cytoprotection Nutrition. Foods and food supply TX341-641 |
spellingShingle |
Antarctic Krill (Euphausia superba) Antioxidant peptide Radical scavenging activity Oxidative stress Cytoprotection Nutrition. Foods and food supply TX341-641 Yue-Zhen Wang Yu-Qin Zhao Yu-Mei Wang Wen-Hao Zhao Peng Wang Chang-Feng Chi Bin Wang Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress |
topic_facet |
Antarctic Krill (Euphausia superba) Antioxidant peptide Radical scavenging activity Oxidative stress Cytoprotection Nutrition. Foods and food supply TX341-641 |
description |
The aim of this study was to purify and identify antioxidant peptides from Antarctic Krill hydrolysate and elucidate their cytoprotection mechanisms on H2O2-induced oxidative stress in Chang liver cells. Using ultrafiltration and chromatographic methods, twelve peptides were purified from the Antarctic Krill hydrolysate and identified as AEK, VEK, VEKT, AEKTR, IEN, VEKGK, LKPGN, IEKG, LQP, ATH, IEKT, and IDSQ. LKPGN and LQP exhibited high scavenging activities on HO·, DPPH·, and O2−·. Moreover, LKPGN and LQP can protect Chang liver cells against H2O2-induced oxidative stress by increasing cell viability and decreasing the apoptosis rate. The mechanism indicated that LKPGN and LQP could concentration-dependently increase the antioxidant enzyme (SOD and GSH-PX) levels to scavenge excess ROS, increase mitochondrial membrane potential, and decrease DNA damage and MDA content. Therefore, LKPGN and LQP from Antarctic Krill could serve as promising candidates applied in health-promoting products. |
format |
Article in Journal/Newspaper |
author |
Yue-Zhen Wang Yu-Qin Zhao Yu-Mei Wang Wen-Hao Zhao Peng Wang Chang-Feng Chi Bin Wang |
author_facet |
Yue-Zhen Wang Yu-Qin Zhao Yu-Mei Wang Wen-Hao Zhao Peng Wang Chang-Feng Chi Bin Wang |
author_sort |
Yue-Zhen Wang |
title |
Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress |
title_short |
Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress |
title_full |
Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress |
title_fullStr |
Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress |
title_full_unstemmed |
Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress |
title_sort |
antioxidant peptides from antarctic krill (euphausia superba) hydrolysate: preparation, identification and cytoprotection on h2o2-induced oxidative stress |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doi.org/10.1016/j.jff.2021.104701 https://doaj.org/article/bb4dbd998d124a98b74857f2703dc812 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_source |
Journal of Functional Foods, Vol 86, Iss , Pp 104701- (2021) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1756464621003509 https://doaj.org/toc/1756-4646 1756-4646 doi:10.1016/j.jff.2021.104701 https://doaj.org/article/bb4dbd998d124a98b74857f2703dc812 |
op_doi |
https://doi.org/10.1016/j.jff.2021.104701 |
container_title |
Journal of Functional Foods |
container_volume |
86 |
container_start_page |
104701 |
_version_ |
1766273257432940544 |