Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa
Mimba leaves (Azadirachta indica A.Juss), which was obtained from Brigade Proteksi Tanaman (BPT) Situbundo in this study, contain active compounds of quercetin-3-O-glucoside (Q3G) and quercetin-4’-O-glucoside (Q4G). These compounds were isolated using the Thin Layer Chromatography (TLC). From previo...
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2016
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ftdoajarticles:oai:doaj.org/article:bb3320dcd86541cb8a7ee61c932f1f0a 2023-05-15T18:05:08+02:00 Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa Enny Rohmawaty 2016-12-01T00:00:00Z https://doi.org/10.15395/mkb.v48n4.913 https://doaj.org/article/bb3320dcd86541cb8a7ee61c932f1f0a EN ID eng ind Universitas Padjajaran http://journal.fk.unpad.ac.id/index.php/mkb/article/view/913 https://doaj.org/toc/0126-074X https://doaj.org/toc/2338-6223 doi:10.15395/mkb.v48n4.913 0126-074X 2338-6223 https://doaj.org/article/bb3320dcd86541cb8a7ee61c932f1f0a Majalah Kedokteran Bandung, Vol 48, Iss 4, Pp 222-227 (2016) Glucose uptake mimba leaves Q3G Q4G Medicine R article 2016 ftdoajarticles https://doi.org/10.15395/mkb.v48n4.913 2022-12-31T00:52:31Z Mimba leaves (Azadirachta indica A.Juss), which was obtained from Brigade Proteksi Tanaman (BPT) Situbundo in this study, contain active compounds of quercetin-3-O-glucoside (Q3G) and quercetin-4’-O-glucoside (Q4G). These compounds were isolated using the Thin Layer Chromatography (TLC). From previous studies, Q3G and Q4G are known to inhibit the glucose uptake from intestinal membrane. This study was conducted to understand the potential influence of Q3G and Q4G isolated from mimba leaves in inhibiting glucose uptake in rat’s intestinal membrane (Rattus rattus norvegicus). This experimental study employed 30 male rats that met the inclusion criteria that were divided into 5 groups (n=6). Group I was the control group and only received glucose solution 3,0 x 10-3 M. Group II, III, IV, and V received glucose solution with Q3G 1 mg/kgBW, Q3G 2 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 1 2 mg/kgBW, respectively. The inhibitory potentials of Q3G and Q4G on glucose uptake was measured every 15 minutes for one hour using in situ perfusion equipment. Data were analyzed using One-way ANOVA and Duncan test with a significance level 95% (α=0.05). From 1 kg fresh mimba leaves, 60.1 mg Q3G and 14.4 mg Q4G were isolated. This study showed that Q3G 1 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 2 mg/kgBW significantly inhibited glucose uptake from rat intestinal membrane compared to negative control at 45th minute (p<0.05). Therefore, Q3G and Q4G isolated from mimba leaves have significantly inhibit glucose uptake from rat’s intestinal membrane (Rattus rattus norvegicus). [MKB. 2016;48(4):222–7] Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles Majalah Kedokteran Bandung 48 4 222 227 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Indonesian |
topic |
Glucose uptake mimba leaves Q3G Q4G Medicine R |
spellingShingle |
Glucose uptake mimba leaves Q3G Q4G Medicine R Enny Rohmawaty Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa |
topic_facet |
Glucose uptake mimba leaves Q3G Q4G Medicine R |
description |
Mimba leaves (Azadirachta indica A.Juss), which was obtained from Brigade Proteksi Tanaman (BPT) Situbundo in this study, contain active compounds of quercetin-3-O-glucoside (Q3G) and quercetin-4’-O-glucoside (Q4G). These compounds were isolated using the Thin Layer Chromatography (TLC). From previous studies, Q3G and Q4G are known to inhibit the glucose uptake from intestinal membrane. This study was conducted to understand the potential influence of Q3G and Q4G isolated from mimba leaves in inhibiting glucose uptake in rat’s intestinal membrane (Rattus rattus norvegicus). This experimental study employed 30 male rats that met the inclusion criteria that were divided into 5 groups (n=6). Group I was the control group and only received glucose solution 3,0 x 10-3 M. Group II, III, IV, and V received glucose solution with Q3G 1 mg/kgBW, Q3G 2 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 1 2 mg/kgBW, respectively. The inhibitory potentials of Q3G and Q4G on glucose uptake was measured every 15 minutes for one hour using in situ perfusion equipment. Data were analyzed using One-way ANOVA and Duncan test with a significance level 95% (α=0.05). From 1 kg fresh mimba leaves, 60.1 mg Q3G and 14.4 mg Q4G were isolated. This study showed that Q3G 1 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 2 mg/kgBW significantly inhibited glucose uptake from rat intestinal membrane compared to negative control at 45th minute (p<0.05). Therefore, Q3G and Q4G isolated from mimba leaves have significantly inhibit glucose uptake from rat’s intestinal membrane (Rattus rattus norvegicus). [MKB. 2016;48(4):222–7] |
format |
Article in Journal/Newspaper |
author |
Enny Rohmawaty |
author_facet |
Enny Rohmawaty |
author_sort |
Enny Rohmawaty |
title |
Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa |
title_short |
Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa |
title_full |
Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa |
title_fullStr |
Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa |
title_full_unstemmed |
Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa |
title_sort |
potensi quercetin-3-o-glucoside (q3g) dan quercetin-4-o-glucoside (q4g) dari daun mimba (azadirachta indica a.juss) terhadap ambilan glukosa |
publisher |
Universitas Padjajaran |
publishDate |
2016 |
url |
https://doi.org/10.15395/mkb.v48n4.913 https://doaj.org/article/bb3320dcd86541cb8a7ee61c932f1f0a |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Majalah Kedokteran Bandung, Vol 48, Iss 4, Pp 222-227 (2016) |
op_relation |
http://journal.fk.unpad.ac.id/index.php/mkb/article/view/913 https://doaj.org/toc/0126-074X https://doaj.org/toc/2338-6223 doi:10.15395/mkb.v48n4.913 0126-074X 2338-6223 https://doaj.org/article/bb3320dcd86541cb8a7ee61c932f1f0a |
op_doi |
https://doi.org/10.15395/mkb.v48n4.913 |
container_title |
Majalah Kedokteran Bandung |
container_volume |
48 |
container_issue |
4 |
container_start_page |
222 |
op_container_end_page |
227 |
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1766176586222010368 |