THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE
The aim of the study was to determine the quantitative and qualitative content of phenolic compounds and flavonoids in Deschampsia antarctica E. Desv. tissue cultures obtained from plants originating from different islands of the maritime Antarctic. Methods. In vitro tissue culture, Folin-Ciocalteu...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2021
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ftdoajarticles:oai:doaj.org/article:bb07b3903fbc48a4a486c76edf53d9af 2023-10-09T21:45:46+02:00 THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE M. O. Twardovska I. I. Konvalyuk K. V. Lystvan I. O. Andreev V. A. Kunakh 2021-04-01T00:00:00Z https://doi.org/10.15407/biotech14.02.059 https://doaj.org/article/bb07b3903fbc48a4a486c76edf53d9af EN UK eng ukr National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry http://biotechnology.kiev.ua/images/storage/2_2021/twardovska_2_2021.pdf https://doaj.org/toc/2410-7751 https://doaj.org/toc/2410-776X doi:10.15407/biotech14.02.059 2410-7751 2410-776X https://doaj.org/article/bb07b3903fbc48a4a486c76edf53d9af Biotechnologia Acta, Vol 14, Iss 2, Pp 59-66 (2021) plant tissue culture deschampsia antarctica e. desv phenolic compounds flavonoids hplc analysis Biotechnology TP248.13-248.65 article 2021 ftdoajarticles https://doi.org/10.15407/biotech14.02.059 2023-09-10T00:51:10Z The aim of the study was to determine the quantitative and qualitative content of phenolic compounds and flavonoids in Deschampsia antarctica E. Desv. tissue cultures obtained from plants originating from different islands of the maritime Antarctic. Methods. In vitro tissue culture, Folin-Ciocalteu method, spectrophotometry, HPLC analysis. Results. The quantitative content of phenolic compounds and flavonoids in D. antarctica tissue cultures obtained from plants of six genotypes (DAR12, DAR13, G/D12-2a, Y66, R30 and L57) was determined. The highest content of phenolic compounds (4.46 and 3.75 mg/g) was found in tissue cultures obtained from root and leaf explants of plant genotype L57. The highest amount of flavonoids (7.17 mg/g) was accumulated in G/D12-2a tissue culture of root origin. The content of the studied biologically active compounds (BACs) did not change with increasing number of subculture generations (from passage 10 to 19). HPLC analysis showed that in D. antarctica tissue cultures, a shift in the biosynthesis of BACs occurred towards the synthesis of more polar metabolites compared to explant donor plants. Conclusions. It was found that the transition of cells to undifferentiated growth affected the content of BACs, the amount of which decreased 2–5 times simultaneously with a significant change in their profile. This provided a basis for further biochemical studies, as well as for careful selection of tissue culture of D. antarctica to use it as a potential source of BACs. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Biotechnologia Acta 14 2 59 66 |
institution |
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collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Ukrainian |
topic |
plant tissue culture deschampsia antarctica e. desv phenolic compounds flavonoids hplc analysis Biotechnology TP248.13-248.65 |
spellingShingle |
plant tissue culture deschampsia antarctica e. desv phenolic compounds flavonoids hplc analysis Biotechnology TP248.13-248.65 M. O. Twardovska I. I. Konvalyuk K. V. Lystvan I. O. Andreev V. A. Kunakh THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE |
topic_facet |
plant tissue culture deschampsia antarctica e. desv phenolic compounds flavonoids hplc analysis Biotechnology TP248.13-248.65 |
description |
The aim of the study was to determine the quantitative and qualitative content of phenolic compounds and flavonoids in Deschampsia antarctica E. Desv. tissue cultures obtained from plants originating from different islands of the maritime Antarctic. Methods. In vitro tissue culture, Folin-Ciocalteu method, spectrophotometry, HPLC analysis. Results. The quantitative content of phenolic compounds and flavonoids in D. antarctica tissue cultures obtained from plants of six genotypes (DAR12, DAR13, G/D12-2a, Y66, R30 and L57) was determined. The highest content of phenolic compounds (4.46 and 3.75 mg/g) was found in tissue cultures obtained from root and leaf explants of plant genotype L57. The highest amount of flavonoids (7.17 mg/g) was accumulated in G/D12-2a tissue culture of root origin. The content of the studied biologically active compounds (BACs) did not change with increasing number of subculture generations (from passage 10 to 19). HPLC analysis showed that in D. antarctica tissue cultures, a shift in the biosynthesis of BACs occurred towards the synthesis of more polar metabolites compared to explant donor plants. Conclusions. It was found that the transition of cells to undifferentiated growth affected the content of BACs, the amount of which decreased 2–5 times simultaneously with a significant change in their profile. This provided a basis for further biochemical studies, as well as for careful selection of tissue culture of D. antarctica to use it as a potential source of BACs. |
format |
Article in Journal/Newspaper |
author |
M. O. Twardovska I. I. Konvalyuk K. V. Lystvan I. O. Andreev V. A. Kunakh |
author_facet |
M. O. Twardovska I. I. Konvalyuk K. V. Lystvan I. O. Andreev V. A. Kunakh |
author_sort |
M. O. Twardovska |
title |
THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE |
title_short |
THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE |
title_full |
THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE |
title_fullStr |
THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE |
title_full_unstemmed |
THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE |
title_sort |
content of phenolic compounds and flavonoids in deschampsia antarctica tissue culture |
publisher |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
publishDate |
2021 |
url |
https://doi.org/10.15407/biotech14.02.059 https://doaj.org/article/bb07b3903fbc48a4a486c76edf53d9af |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Biotechnologia Acta, Vol 14, Iss 2, Pp 59-66 (2021) |
op_relation |
http://biotechnology.kiev.ua/images/storage/2_2021/twardovska_2_2021.pdf https://doaj.org/toc/2410-7751 https://doaj.org/toc/2410-776X doi:10.15407/biotech14.02.059 2410-7751 2410-776X https://doaj.org/article/bb07b3903fbc48a4a486c76edf53d9af |
op_doi |
https://doi.org/10.15407/biotech14.02.059 |
container_title |
Biotechnologia Acta |
container_volume |
14 |
container_issue |
2 |
container_start_page |
59 |
op_container_end_page |
66 |
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1779321419246600192 |