Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression
Objective: To explore cytotoxicity of Synsepalum dulcificum (S. dulcificum) Daniell (Sapotaceae) on human colon cancer (HCT-116 and HT-29), human monocytic leukemia (THP-1) and normal (HDFn) cell lines, and its effect on the expression of early apoptotic genes, c-fos and c-jun. Methods: Leaf, stem a...
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Wolters Kluwer Medknow Publications
2018
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Online Access: | https://doi.org/10.4103/2221-1691.227999 https://doaj.org/article/bae7520033ce4669adde9c8dcdf4da2a |
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ftdoajarticles:oai:doaj.org/article:bae7520033ce4669adde9c8dcdf4da2a 2023-05-15T15:11:54+02:00 Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression Jichang Seong Glenn G Oyong Esperanza C Cabrera 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.227999 https://doaj.org/article/bae7520033ce4669adde9c8dcdf4da2a EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=3;spage=173;epage=178;aulast=Seong https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.227999 https://doaj.org/article/bae7520033ce4669adde9c8dcdf4da2a Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 3, Pp 173-178 (2018) synsepalum dulcificum colon cancer monocytic leukemia apoptosis c-fos c-jun Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.227999 2022-12-31T10:30:46Z Objective: To explore cytotoxicity of Synsepalum dulcificum (S. dulcificum) Daniell (Sapotaceae) on human colon cancer (HCT-116 and HT-29), human monocytic leukemia (THP-1) and normal (HDFn) cell lines, and its effect on the expression of early apoptotic genes, c-fos and c-jun. Methods: Leaf, stem and berry of S. dulcificum were separately extracted by using 2 solvents, 10% ethanol (EtOH) and 80% methanol (MeOH). PrestoBlue® cell viability assay and qRT-PCR assay were conducted to examine the above objectives respectively. Results: Stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum were cytotoxic to HCT-116 and HT-29 human colon cancer cells. For HCT-116, IC50 values of these 3 extracts were not significantly different (P>0.05) from that of the positive control bleomycin (IC50 of 33.57 μg/mL), while for HT-29, IC50 values of these 3 extracts were significantly lower (P<0.05) than that of bleomycin (IC50 of 25.24 μg/mL). None of the extracts were cytotoxic to the THP-1 monocytic leukemia cells and HDFn normal human dermal fibroblasts. For both HCT-116 and HT-29, these extracts significantly up-regulated (P<0.05) the expression of c-fos and c-jun compared to the untreated negative control. Conclusions: The results of this study suggest that cytotoxicity of stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum on HCT-116 and HT-29 colon cancer cells is due to the induced apoptosis which is caused by the up-regulation of the expression of early apoptotic genes, c-fos and c-jun. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Daniell ENVELOPE(169.917,169.917,-72.717,-72.717) Asian Pacific Journal of Tropical Biomedicine 8 3 173 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
synsepalum dulcificum colon cancer monocytic leukemia apoptosis c-fos c-jun Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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synsepalum dulcificum colon cancer monocytic leukemia apoptosis c-fos c-jun Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Jichang Seong Glenn G Oyong Esperanza C Cabrera Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression |
topic_facet |
synsepalum dulcificum colon cancer monocytic leukemia apoptosis c-fos c-jun Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To explore cytotoxicity of Synsepalum dulcificum (S. dulcificum) Daniell (Sapotaceae) on human colon cancer (HCT-116 and HT-29), human monocytic leukemia (THP-1) and normal (HDFn) cell lines, and its effect on the expression of early apoptotic genes, c-fos and c-jun. Methods: Leaf, stem and berry of S. dulcificum were separately extracted by using 2 solvents, 10% ethanol (EtOH) and 80% methanol (MeOH). PrestoBlue® cell viability assay and qRT-PCR assay were conducted to examine the above objectives respectively. Results: Stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum were cytotoxic to HCT-116 and HT-29 human colon cancer cells. For HCT-116, IC50 values of these 3 extracts were not significantly different (P>0.05) from that of the positive control bleomycin (IC50 of 33.57 μg/mL), while for HT-29, IC50 values of these 3 extracts were significantly lower (P<0.05) than that of bleomycin (IC50 of 25.24 μg/mL). None of the extracts were cytotoxic to the THP-1 monocytic leukemia cells and HDFn normal human dermal fibroblasts. For both HCT-116 and HT-29, these extracts significantly up-regulated (P<0.05) the expression of c-fos and c-jun compared to the untreated negative control. Conclusions: The results of this study suggest that cytotoxicity of stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum on HCT-116 and HT-29 colon cancer cells is due to the induced apoptosis which is caused by the up-regulation of the expression of early apoptotic genes, c-fos and c-jun. |
format |
Article in Journal/Newspaper |
author |
Jichang Seong Glenn G Oyong Esperanza C Cabrera |
author_facet |
Jichang Seong Glenn G Oyong Esperanza C Cabrera |
author_sort |
Jichang Seong |
title |
Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression |
title_short |
Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression |
title_full |
Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression |
title_fullStr |
Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression |
title_full_unstemmed |
Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression |
title_sort |
synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.227999 https://doaj.org/article/bae7520033ce4669adde9c8dcdf4da2a |
long_lat |
ENVELOPE(169.917,169.917,-72.717,-72.717) |
geographic |
Arctic Daniell |
geographic_facet |
Arctic Daniell |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 3, Pp 173-178 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=3;spage=173;epage=178;aulast=Seong https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.227999 https://doaj.org/article/bae7520033ce4669adde9c8dcdf4da2a |
op_doi |
https://doi.org/10.4103/2221-1691.227999 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
3 |
container_start_page |
173 |
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1766342675674431488 |