Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice
Abstract Background Virus-like particles have been regularly used as an antigen delivery system for a number of Plasmodium peptides or proteins. The present study reports the immunogenicity and protective efficacy of bacterium-like particles (BLPs) generated from Lactococcus lactis and loaded with P...
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ftdoajarticles:oai:doaj.org/article:baa76770997d47e3ae28563d9ac3a81d 2023-05-15T15:11:26+02:00 Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice Nganou-Makamdop Krystelle van Roosmalen Maarten L Audouy Sandrine AL van Gemert Geert-Jan Leenhouts Kees Hermsen Cornelus C Sauerwein Robert W 2012-02-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-50 https://doaj.org/article/baa76770997d47e3ae28563d9ac3a81d EN eng BMC http://www.malariajournal.com/content/11/1/50 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-50 1475-2875 https://doaj.org/article/baa76770997d47e3ae28563d9ac3a81d Malaria Journal, Vol 11, Iss 1, p 50 (2012) BLP CSP Delivery platform Immunization Malaria Plasmodium berghei Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-50 2022-12-31T08:36:01Z Abstract Background Virus-like particles have been regularly used as an antigen delivery system for a number of Plasmodium peptides or proteins. The present study reports the immunogenicity and protective efficacy of bacterium-like particles (BLPs) generated from Lactococcus lactis and loaded with Plasmodium berghei circumsporozoite protein (PbCSP) peptides. Methods A panel of BLP-PbCSP formulations differing in composition and quantity of B-cell, CD4+ and CD8+ T-cell epitopes of PbCSP were tested in BALB/c mice. Results BLP-PbCSP1 induced specific humoral responses but no IFN-γ ELISPOT response, protecting 30-40% of the immunized mice. BLP-PbCSP2, with reduced length of the non-immunogenic part of the T-cell-epitopes construct, increased induction of IFN-γ responses as well as protection up to 60-70%. Compared to controls, lower parasitaemia was observed in unprotected mice immunized with BLP-PbCSP1 or 2, suggestive for partial immunity. Finally, further increase of the number of B-cell epitopes and codon optimization (BLP-PbCSP4) induced the highest anti-CSP antibody levels and number of IFN-γ spots, resulting in sterile immunity in 100% of the immunized mice. Conclusion Presentation of Plasmodium -derived antigens using BLPs as a delivery system induced complete protection in a murine malaria model. Eventually, BLPs have the potential to be used as a novel versatile delivery platform in malaria vaccine development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 50 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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BLP CSP Delivery platform Immunization Malaria Plasmodium berghei Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
BLP CSP Delivery platform Immunization Malaria Plasmodium berghei Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Nganou-Makamdop Krystelle van Roosmalen Maarten L Audouy Sandrine AL van Gemert Geert-Jan Leenhouts Kees Hermsen Cornelus C Sauerwein Robert W Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice |
topic_facet |
BLP CSP Delivery platform Immunization Malaria Plasmodium berghei Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Virus-like particles have been regularly used as an antigen delivery system for a number of Plasmodium peptides or proteins. The present study reports the immunogenicity and protective efficacy of bacterium-like particles (BLPs) generated from Lactococcus lactis and loaded with Plasmodium berghei circumsporozoite protein (PbCSP) peptides. Methods A panel of BLP-PbCSP formulations differing in composition and quantity of B-cell, CD4+ and CD8+ T-cell epitopes of PbCSP were tested in BALB/c mice. Results BLP-PbCSP1 induced specific humoral responses but no IFN-γ ELISPOT response, protecting 30-40% of the immunized mice. BLP-PbCSP2, with reduced length of the non-immunogenic part of the T-cell-epitopes construct, increased induction of IFN-γ responses as well as protection up to 60-70%. Compared to controls, lower parasitaemia was observed in unprotected mice immunized with BLP-PbCSP1 or 2, suggestive for partial immunity. Finally, further increase of the number of B-cell epitopes and codon optimization (BLP-PbCSP4) induced the highest anti-CSP antibody levels and number of IFN-γ spots, resulting in sterile immunity in 100% of the immunized mice. Conclusion Presentation of Plasmodium -derived antigens using BLPs as a delivery system induced complete protection in a murine malaria model. Eventually, BLPs have the potential to be used as a novel versatile delivery platform in malaria vaccine development. |
format |
Article in Journal/Newspaper |
author |
Nganou-Makamdop Krystelle van Roosmalen Maarten L Audouy Sandrine AL van Gemert Geert-Jan Leenhouts Kees Hermsen Cornelus C Sauerwein Robert W |
author_facet |
Nganou-Makamdop Krystelle van Roosmalen Maarten L Audouy Sandrine AL van Gemert Geert-Jan Leenhouts Kees Hermsen Cornelus C Sauerwein Robert W |
author_sort |
Nganou-Makamdop Krystelle |
title |
Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice |
title_short |
Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice |
title_full |
Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice |
title_fullStr |
Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice |
title_full_unstemmed |
Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice |
title_sort |
bacterium-like particles as multi-epitope delivery platform for plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-50 https://doaj.org/article/baa76770997d47e3ae28563d9ac3a81d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 50 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/50 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-50 1475-2875 https://doaj.org/article/baa76770997d47e3ae28563d9ac3a81d |
op_doi |
https://doi.org/10.1186/1475-2875-11-50 |
container_title |
Malaria Journal |
container_volume |
11 |
container_issue |
1 |
container_start_page |
50 |
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1766342287683485696 |