Vertical transmission of zika virus in Aedes albopictus.
Background Zika virus (ZIKV) is an arthropod-borne flavivirus transmitted by Aedes mosquitoes. Aedes albopictus is an important vector of ZIKV worldwide. To date, most experiments have focused on the vertical transmission of ZIKV in Ae. aegypti, while studies on Ae. albopictus are very limited. To e...
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ftdoajarticles:oai:doaj.org/article:ba982e9b78074c70bb56767b7fa24732 2023-05-15T15:12:33+02:00 Vertical transmission of zika virus in Aedes albopictus. Zetian Lai Tengfei Zhou Jiayong Zhou Shuang Liu Ye Xu Jinbao Gu Guiyun Yan Xiao-Guang Chen 2020-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008776 https://doaj.org/article/ba982e9b78074c70bb56767b7fa24732 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008776 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008776 https://doaj.org/article/ba982e9b78074c70bb56767b7fa24732 PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008776 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008776 2022-12-31T05:05:53Z Background Zika virus (ZIKV) is an arthropod-borne flavivirus transmitted by Aedes mosquitoes. Aedes albopictus is an important vector of ZIKV worldwide. To date, most experiments have focused on the vertical transmission of ZIKV in Ae. aegypti, while studies on Ae. albopictus are very limited. To explore vertical transmission in Ae. albopictus, a series of laboratory studies were carried out. Methodology/principal findings In this study, Ae. albopictus were blood-fed with ZIKV-infectious blood, and the ovaries and offspring viral infection rates were analyzed by reverse transcription PCR (RT-PCR), real-time reverse transcription PCR (RT-qPCR) and immunohistochemistry (IHC). ZIKV was detected in the ovaries and oviposited eggs in two gonotrophic cycles. The minimum filial egg infection rates in two gonotrophic cycles were 2.06% and 0.69%, and the effective population transmission rate was 1.87%. The hatching, pupation, and emergence rates of infected offspring were not significantly different from those of uninfected offspring, indicating that ZIKV did not prevent the offspring from completing the growth and development process. ZIKV was detected in three of thirteen C57BL/6 suckling mice bitten by ZIKV-positive F1 females, and the viremia persisted for at least seven days. Conclusions/significance ZIKV can be vertically transmitted in Ae. albopictus via transovarial transmission. The vertical transmission rates in F1 eggs and adults were 2.06% and 1.87%, respectively. Even though the vertical transmission rates were low, the female mosquitoes infected via the congenital route horizontally transmitted ZIKV to suckling mice through bloodsucking. This is the first experimental evidence of offspring with vertically transmitted ZIKV initiating new horizontal transmission. The present study deepens the understanding of the vertical transmission of flaviviruses in Aedes mosquitoes and sheds light on the prevention and control of mosquito-borne diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 10 e0008776 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Zetian Lai Tengfei Zhou Jiayong Zhou Shuang Liu Ye Xu Jinbao Gu Guiyun Yan Xiao-Guang Chen Vertical transmission of zika virus in Aedes albopictus. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Zika virus (ZIKV) is an arthropod-borne flavivirus transmitted by Aedes mosquitoes. Aedes albopictus is an important vector of ZIKV worldwide. To date, most experiments have focused on the vertical transmission of ZIKV in Ae. aegypti, while studies on Ae. albopictus are very limited. To explore vertical transmission in Ae. albopictus, a series of laboratory studies were carried out. Methodology/principal findings In this study, Ae. albopictus were blood-fed with ZIKV-infectious blood, and the ovaries and offspring viral infection rates were analyzed by reverse transcription PCR (RT-PCR), real-time reverse transcription PCR (RT-qPCR) and immunohistochemistry (IHC). ZIKV was detected in the ovaries and oviposited eggs in two gonotrophic cycles. The minimum filial egg infection rates in two gonotrophic cycles were 2.06% and 0.69%, and the effective population transmission rate was 1.87%. The hatching, pupation, and emergence rates of infected offspring were not significantly different from those of uninfected offspring, indicating that ZIKV did not prevent the offspring from completing the growth and development process. ZIKV was detected in three of thirteen C57BL/6 suckling mice bitten by ZIKV-positive F1 females, and the viremia persisted for at least seven days. Conclusions/significance ZIKV can be vertically transmitted in Ae. albopictus via transovarial transmission. The vertical transmission rates in F1 eggs and adults were 2.06% and 1.87%, respectively. Even though the vertical transmission rates were low, the female mosquitoes infected via the congenital route horizontally transmitted ZIKV to suckling mice through bloodsucking. This is the first experimental evidence of offspring with vertically transmitted ZIKV initiating new horizontal transmission. The present study deepens the understanding of the vertical transmission of flaviviruses in Aedes mosquitoes and sheds light on the prevention and control of mosquito-borne diseases. |
format |
Article in Journal/Newspaper |
author |
Zetian Lai Tengfei Zhou Jiayong Zhou Shuang Liu Ye Xu Jinbao Gu Guiyun Yan Xiao-Guang Chen |
author_facet |
Zetian Lai Tengfei Zhou Jiayong Zhou Shuang Liu Ye Xu Jinbao Gu Guiyun Yan Xiao-Guang Chen |
author_sort |
Zetian Lai |
title |
Vertical transmission of zika virus in Aedes albopictus. |
title_short |
Vertical transmission of zika virus in Aedes albopictus. |
title_full |
Vertical transmission of zika virus in Aedes albopictus. |
title_fullStr |
Vertical transmission of zika virus in Aedes albopictus. |
title_full_unstemmed |
Vertical transmission of zika virus in Aedes albopictus. |
title_sort |
vertical transmission of zika virus in aedes albopictus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008776 https://doaj.org/article/ba982e9b78074c70bb56767b7fa24732 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008776 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008776 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008776 https://doaj.org/article/ba982e9b78074c70bb56767b7fa24732 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008776 |
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PLOS Neglected Tropical Diseases |
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14 |
container_issue |
10 |
container_start_page |
e0008776 |
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