An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens.
The global spread of Zika virus (ZIKV), which caused a pandemic associated with Congenital Zika Syndrome and neuropathology in newborns and adults, prompted the pursuit of a safe and effective vaccine. Here, three kinds of recombinant rabies virus (RABV) encoding the prM-E protein of ZIKV were const...
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ftdoajarticles:oai:doaj.org/article:ba885491555e46738a09e5d5f956d0ae 2023-05-15T15:12:50+02:00 An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens. Hongli Jin Cuicui Jiao Zengguo Cao Pei Huang Hang Chi Yujie Bai Di Liu Jianzhong Wang Na Feng Nan Li Yongkun Zhao Tiecheng Wang Yuwei Gao Songtao Yang Xianzhu Xia Hualei Wang 2021-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009484 https://doaj.org/article/ba885491555e46738a09e5d5f956d0ae EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009484 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009484 https://doaj.org/article/ba885491555e46738a09e5d5f956d0ae PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009484 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009484 2022-12-31T09:08:25Z The global spread of Zika virus (ZIKV), which caused a pandemic associated with Congenital Zika Syndrome and neuropathology in newborns and adults, prompted the pursuit of a safe and effective vaccine. Here, three kinds of recombinant rabies virus (RABV) encoding the prM-E protein of ZIKV were constructed: ZI-D (prM-E), ZI-E (transmembrane domain (TM) of prM-E replaced with RABV G) and ZI-F (signal peptide and TM domain of prM-E replaced with the region of RABV G). When the TM of prM-E was replaced with the region of RABV G (termed ZI-E), it promoted ZIKV E protein localization on the cell membrane and assembly on recombinant viruses. In addition, the change in the signal peptide with RABV G (termed ZI-F) was not conducive to foreign protein expression. The immunogenicity of recombinant viruses mixed with a complex adjuvant of ISA 201 VG and poly(I:C) was tested in BALB/c mice. After immunization with ZI-E, the anti-ZIKV IgG antibody lasted for at least 10 weeks. The titers of neutralizing antibodies (NAbs) against ZIKV and RABV at week 6 were all greater than the protective titers. Moreover, ZI-E stimulated the proliferation of splenic lymphocytes and promoted the secretion of cytokines. It also promoted the production of central memory T cells (TCMs) among CD4+/CD8+ T cells and stimulated B cell activation and maturation. These results indicate that ZI-E could induce ZIKV-specific humoral and cellular immune responses, which have the potential to be developed into a promising vaccine for protection against both ZIKV and RABV infections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 6 e0009484 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hongli Jin Cuicui Jiao Zengguo Cao Pei Huang Hang Chi Yujie Bai Di Liu Jianzhong Wang Na Feng Nan Li Yongkun Zhao Tiecheng Wang Yuwei Gao Songtao Yang Xianzhu Xia Hualei Wang An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The global spread of Zika virus (ZIKV), which caused a pandemic associated with Congenital Zika Syndrome and neuropathology in newborns and adults, prompted the pursuit of a safe and effective vaccine. Here, three kinds of recombinant rabies virus (RABV) encoding the prM-E protein of ZIKV were constructed: ZI-D (prM-E), ZI-E (transmembrane domain (TM) of prM-E replaced with RABV G) and ZI-F (signal peptide and TM domain of prM-E replaced with the region of RABV G). When the TM of prM-E was replaced with the region of RABV G (termed ZI-E), it promoted ZIKV E protein localization on the cell membrane and assembly on recombinant viruses. In addition, the change in the signal peptide with RABV G (termed ZI-F) was not conducive to foreign protein expression. The immunogenicity of recombinant viruses mixed with a complex adjuvant of ISA 201 VG and poly(I:C) was tested in BALB/c mice. After immunization with ZI-E, the anti-ZIKV IgG antibody lasted for at least 10 weeks. The titers of neutralizing antibodies (NAbs) against ZIKV and RABV at week 6 were all greater than the protective titers. Moreover, ZI-E stimulated the proliferation of splenic lymphocytes and promoted the secretion of cytokines. It also promoted the production of central memory T cells (TCMs) among CD4+/CD8+ T cells and stimulated B cell activation and maturation. These results indicate that ZI-E could induce ZIKV-specific humoral and cellular immune responses, which have the potential to be developed into a promising vaccine for protection against both ZIKV and RABV infections. |
format |
Article in Journal/Newspaper |
author |
Hongli Jin Cuicui Jiao Zengguo Cao Pei Huang Hang Chi Yujie Bai Di Liu Jianzhong Wang Na Feng Nan Li Yongkun Zhao Tiecheng Wang Yuwei Gao Songtao Yang Xianzhu Xia Hualei Wang |
author_facet |
Hongli Jin Cuicui Jiao Zengguo Cao Pei Huang Hang Chi Yujie Bai Di Liu Jianzhong Wang Na Feng Nan Li Yongkun Zhao Tiecheng Wang Yuwei Gao Songtao Yang Xianzhu Xia Hualei Wang |
author_sort |
Hongli Jin |
title |
An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens. |
title_short |
An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens. |
title_full |
An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens. |
title_fullStr |
An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens. |
title_full_unstemmed |
An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens. |
title_sort |
inactivated recombinant rabies virus displaying the zika virus prm-e induces protective immunity against both pathogens. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009484 https://doaj.org/article/ba885491555e46738a09e5d5f956d0ae |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009484 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009484 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009484 https://doaj.org/article/ba885491555e46738a09e5d5f956d0ae |
op_doi |
https://doi.org/10.1371/journal.pntd.0009484 |
container_title |
PLOS Neglected Tropical Diseases |
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15 |
container_issue |
6 |
container_start_page |
e0009484 |
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1766343470110212096 |