A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This...
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ftdoajarticles:oai:doaj.org/article:ba846b904c004f5bbc3d422aa7cec733 2023-05-15T15:57:44+02:00 A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas Conghui Liu Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Weilin Wang Lingling Wang Linsheng Song 2020-07-01T00:00:00Z https://doi.org/10.3389/fimmu.2020.01379 https://doaj.org/article/ba846b904c004f5bbc3d422aa7cec733 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fimmu.2020.01379/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.01379 https://doaj.org/article/ba846b904c004f5bbc3d422aa7cec733 Frontiers in Immunology, Vol 11 (2020) tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Immunologic diseases. Allergy RC581-607 article 2020 ftdoajarticles https://doi.org/10.3389/fimmu.2020.01379 2022-12-31T03:47:47Z Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This work makes contributions by identifying CgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence of CgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p < 0.01) upregulated after the injection of Vibrio splendidus. CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to the Yarrowia lipolytica-containing phagosomes after the stimulation of Y. lipolytica. The recombinant CgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes toward V. splendidus and Y. lipolytica is significantly inhibited (p < 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests that CgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing in C. gigas. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Immunology 11 |
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ftdoajarticles |
language |
English |
topic |
tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Immunologic diseases. Allergy RC581-607 |
spellingShingle |
tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Immunologic diseases. Allergy RC581-607 Conghui Liu Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Weilin Wang Lingling Wang Linsheng Song A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
topic_facet |
tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Immunologic diseases. Allergy RC581-607 |
description |
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This work makes contributions by identifying CgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence of CgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p < 0.01) upregulated after the injection of Vibrio splendidus. CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to the Yarrowia lipolytica-containing phagosomes after the stimulation of Y. lipolytica. The recombinant CgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes toward V. splendidus and Y. lipolytica is significantly inhibited (p < 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests that CgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing in C. gigas. |
format |
Article in Journal/Newspaper |
author |
Conghui Liu Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Weilin Wang Lingling Wang Linsheng Song |
author_facet |
Conghui Liu Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Weilin Wang Lingling Wang Linsheng Song |
author_sort |
Conghui Liu |
title |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
title_short |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
title_full |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
title_fullStr |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
title_full_unstemmed |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas |
title_sort |
cd63 homolog specially recruited to the fungi-contained phagosomes is involved in the cellular immune response of oyster crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.01379 https://doaj.org/article/ba846b904c004f5bbc3d422aa7cec733 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Immunology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fimmu.2020.01379/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.01379 https://doaj.org/article/ba846b904c004f5bbc3d422aa7cec733 |
op_doi |
https://doi.org/10.3389/fimmu.2020.01379 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
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1766393430151266304 |