Genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile Atlantic salmon (Salmo salar)

Abstract Background One objective of this study was to identify putative quantitative trait loci (QTL) that affect indicator phenotypes for growth, nitrogen, and carbon metabolism in muscle, liver, and adipose tissue, and for feed efficiency. Another objective was to perform an RNAseq analysis (184...

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Published in:Genetics Selection Evolution
Main Authors: Hanne Dvergedal, Thomas Nelson Harvey, Yang Jin, Jørgen Ødegård, Lars Grønvold, Simen Rød Sandve, Dag Inge Våge, Thomas Moen, Gunnar Klemetsdal
Format: Article in Journal/Newspaper
Language:German
English
French
Published: BMC 2020
Subjects:
Online Access:https://doi.org/10.1186/s12711-020-00587-x
https://doaj.org/article/ba174ed5290a492d8aa8f9317fad72aa
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spelling ftdoajarticles:oai:doaj.org/article:ba174ed5290a492d8aa8f9317fad72aa 2023-05-15T15:33:02+02:00 Genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile Atlantic salmon (Salmo salar) Hanne Dvergedal Thomas Nelson Harvey Yang Jin Jørgen Ødegård Lars Grønvold Simen Rød Sandve Dag Inge Våge Thomas Moen Gunnar Klemetsdal 2020-11-01T00:00:00Z https://doi.org/10.1186/s12711-020-00587-x https://doaj.org/article/ba174ed5290a492d8aa8f9317fad72aa DE EN FR ger eng fre BMC http://link.springer.com/article/10.1186/s12711-020-00587-x https://doaj.org/toc/1297-9686 doi:10.1186/s12711-020-00587-x 1297-9686 https://doaj.org/article/ba174ed5290a492d8aa8f9317fad72aa Genetics Selection Evolution, Vol 52, Iss 1, Pp 1-15 (2020) Animal culture SF1-1100 Genetics QH426-470 article 2020 ftdoajarticles https://doi.org/10.1186/s12711-020-00587-x 2022-12-31T03:15:03Z Abstract Background One objective of this study was to identify putative quantitative trait loci (QTL) that affect indicator phenotypes for growth, nitrogen, and carbon metabolism in muscle, liver, and adipose tissue, and for feed efficiency. Another objective was to perform an RNAseq analysis (184 fish from all families), to identify genes that are associated with carbon and nitrogen metabolism in the liver. The material consisted of a family experiment that was performed in freshwater and included 2281 individuals from 23 full-sib families. During the 12-day feed conversion test, families were randomly allocated to family tanks (50 fish per tank and 2 tanks per family) and fed a fishmeal-based diet labeled with the stable isotopes 15N and 13C at inclusion levels of 2 and 1%, respectively. Results Using a linear mixed-model algorithm, a QTL for pre-smolt growth was identified on chromosome 9 and a QTL for carbon metabolism in the liver was identified on chromosome 12 that was closely related to feed conversion ratio on a tank level. For the indicators of feed efficiency traits that were derived from the stable isotope ratios (15N and 13C) of muscle tissue and growth, no convincing QTL was detected, which suggests that these traits are polygenic. The transcriptomic analysis showed that high carbon and nitrogen metabolism was associated with individuals that convert protein from the feed more efficiently, primarily due to higher expression of the proteasome, lipid, and carbon metabolic pathways in liver. In addition, we identified seven transcription factors that were associated with carbon and nitrogen metabolism and located in the identified QTL regions. Conclusions Analyses revealed one QTL associated with pre-smolt growth and one QTL for carbon metabolism in the liver. Both of these traits are associated with feed efficiency. However, more accurate mapping of the putative QTL will require a more diverse family material. In this experiment, fish that have a high carbon and nitrogen metabolism in the liver ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Genetics Selection Evolution 52 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language German
English
French
topic Animal culture
SF1-1100
Genetics
QH426-470
spellingShingle Animal culture
SF1-1100
Genetics
QH426-470
Hanne Dvergedal
Thomas Nelson Harvey
Yang Jin
Jørgen Ødegård
Lars Grønvold
Simen Rød Sandve
Dag Inge Våge
Thomas Moen
Gunnar Klemetsdal
Genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile Atlantic salmon (Salmo salar)
topic_facet Animal culture
SF1-1100
Genetics
QH426-470
description Abstract Background One objective of this study was to identify putative quantitative trait loci (QTL) that affect indicator phenotypes for growth, nitrogen, and carbon metabolism in muscle, liver, and adipose tissue, and for feed efficiency. Another objective was to perform an RNAseq analysis (184 fish from all families), to identify genes that are associated with carbon and nitrogen metabolism in the liver. The material consisted of a family experiment that was performed in freshwater and included 2281 individuals from 23 full-sib families. During the 12-day feed conversion test, families were randomly allocated to family tanks (50 fish per tank and 2 tanks per family) and fed a fishmeal-based diet labeled with the stable isotopes 15N and 13C at inclusion levels of 2 and 1%, respectively. Results Using a linear mixed-model algorithm, a QTL for pre-smolt growth was identified on chromosome 9 and a QTL for carbon metabolism in the liver was identified on chromosome 12 that was closely related to feed conversion ratio on a tank level. For the indicators of feed efficiency traits that were derived from the stable isotope ratios (15N and 13C) of muscle tissue and growth, no convincing QTL was detected, which suggests that these traits are polygenic. The transcriptomic analysis showed that high carbon and nitrogen metabolism was associated with individuals that convert protein from the feed more efficiently, primarily due to higher expression of the proteasome, lipid, and carbon metabolic pathways in liver. In addition, we identified seven transcription factors that were associated with carbon and nitrogen metabolism and located in the identified QTL regions. Conclusions Analyses revealed one QTL associated with pre-smolt growth and one QTL for carbon metabolism in the liver. Both of these traits are associated with feed efficiency. However, more accurate mapping of the putative QTL will require a more diverse family material. In this experiment, fish that have a high carbon and nitrogen metabolism in the liver ...
format Article in Journal/Newspaper
author Hanne Dvergedal
Thomas Nelson Harvey
Yang Jin
Jørgen Ødegård
Lars Grønvold
Simen Rød Sandve
Dag Inge Våge
Thomas Moen
Gunnar Klemetsdal
author_facet Hanne Dvergedal
Thomas Nelson Harvey
Yang Jin
Jørgen Ødegård
Lars Grønvold
Simen Rød Sandve
Dag Inge Våge
Thomas Moen
Gunnar Klemetsdal
author_sort Hanne Dvergedal
title Genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile Atlantic salmon (Salmo salar)
title_short Genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile Atlantic salmon (Salmo salar)
title_full Genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile Atlantic salmon (Salmo salar)
title_fullStr Genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile Atlantic salmon (Salmo salar)
title_full_unstemmed Genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile Atlantic salmon (Salmo salar)
title_sort genomic regions and signaling pathways associated with indicator traits for feed efficiency in juvenile atlantic salmon (salmo salar)
publisher BMC
publishDate 2020
url https://doi.org/10.1186/s12711-020-00587-x
https://doaj.org/article/ba174ed5290a492d8aa8f9317fad72aa
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_source Genetics Selection Evolution, Vol 52, Iss 1, Pp 1-15 (2020)
op_relation http://link.springer.com/article/10.1186/s12711-020-00587-x
https://doaj.org/toc/1297-9686
doi:10.1186/s12711-020-00587-x
1297-9686
https://doaj.org/article/ba174ed5290a492d8aa8f9317fad72aa
op_doi https://doi.org/10.1186/s12711-020-00587-x
container_title Genetics Selection Evolution
container_volume 52
container_issue 1
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