Antioxidant and anti-stress properties of postbiotics produced by Lysinibacillus macroides G117
In this study, Lysinibacillus macroides G117 was isolated from vinegar residue, and the probiotic activity of its postbiotics was determined in Artemia salina and Scophthalmus maximus. First, the functional genome of L. macroides G117 was analysed. The total length of the genome was 4,728,501 bp, co...
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ftdoajarticles:oai:doaj.org/article:b9f6491c928a4736977bc760ee4341ed 2024-09-15T18:34:01+00:00 Antioxidant and anti-stress properties of postbiotics produced by Lysinibacillus macroides G117 Shuo Qiao Chengjie Lv Xiaoli Zhang Xiaojing Lv Dinglong Yang Jianmin Zhao 2024-06-01T00:00:00Z https://doi.org/10.1016/j.cirep.2024.200143 https://doaj.org/article/b9f6491c928a4736977bc760ee4341ed EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2950311624000107 https://doaj.org/toc/2950-3116 2950-3116 doi:10.1016/j.cirep.2024.200143 https://doaj.org/article/b9f6491c928a4736977bc760ee4341ed Comparative Immunology Reports, Vol 6, Iss , Pp 200143- (2024) Lysinibacillus macroides g117 Postbiotics Artemia salina Scophthalmus maximus antioxidant activities Microbiology QR1-502 Veterinary medicine SF600-1100 article 2024 ftdoajarticles https://doi.org/10.1016/j.cirep.2024.200143 2024-08-05T17:49:11Z In this study, Lysinibacillus macroides G117 was isolated from vinegar residue, and the probiotic activity of its postbiotics was determined in Artemia salina and Scophthalmus maximus. First, the functional genome of L. macroides G117 was analysed. The total length of the genome was 4,728,501 bp, composed of one ring chromosome and two ring plasmids. Two gene clusters, β-lactone and type III polyketide synthases (T3PKS), well-known for anti-toxic, anti-inflammatory, antioxidant, anticancer activities, were predicted through the antiSMASH database. In addition, probiotic genes for antioxidants, stress resistance, and anaerobic respiration were identified using the KEGG database. These results suggest that L. macroides G117 could be used as a probiotic in aquaculture. To further assess the probiotic properties of L. macroides G117, postbiotics were cultured and collected. Based on the survival rate of pesticide-stimulated A. salina, the optimum culture condition of L. macroides G117 was analysed. The optimal culture conditions were 1 % sucrose, 1.5 % yeast extract, 0.1 % MgSO4·7H2O, 30 °C, pH 7.8, bottled fluid volume 100 mL, shaking speed 180 rpm, inoculation quantity 0.5 %, and fermentation for 48 h, with a 15 % increase in survival rate of A. salina with the addition of optimised postbiotics. Untargeted metabolomic analysis revealed significant differences between the non-optimised and optimised postbiotics, mainly in amino acid and carbohydrate metabolites. Notably, postbiotics significantly increased the antioxidant enzymes in A. salina (catalase (CAT) and total superoxide dismutase (T-SOD)) and S. maximus (T-SOD) and decreased malondialdehyde (MDA) content. In addition, the activities of anaerobic respiratory-related enzymes (lactate dehydrogenase (LDH) and pyruvate kinase (PK)) were significantly increased, whereas those of aerobic respiratory-related enzymes (malate dehydrogenase (MDH)) were significantly decreased in S. maximus. These results suggest that L. macroides G117 postbiotics could improve the ... Article in Journal/Newspaper Scophthalmus maximus Directory of Open Access Journals: DOAJ Articles Comparative Immunology Reports 6 200143 |
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topic |
Lysinibacillus macroides g117 Postbiotics Artemia salina Scophthalmus maximus antioxidant activities Microbiology QR1-502 Veterinary medicine SF600-1100 |
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Lysinibacillus macroides g117 Postbiotics Artemia salina Scophthalmus maximus antioxidant activities Microbiology QR1-502 Veterinary medicine SF600-1100 Shuo Qiao Chengjie Lv Xiaoli Zhang Xiaojing Lv Dinglong Yang Jianmin Zhao Antioxidant and anti-stress properties of postbiotics produced by Lysinibacillus macroides G117 |
topic_facet |
Lysinibacillus macroides g117 Postbiotics Artemia salina Scophthalmus maximus antioxidant activities Microbiology QR1-502 Veterinary medicine SF600-1100 |
description |
In this study, Lysinibacillus macroides G117 was isolated from vinegar residue, and the probiotic activity of its postbiotics was determined in Artemia salina and Scophthalmus maximus. First, the functional genome of L. macroides G117 was analysed. The total length of the genome was 4,728,501 bp, composed of one ring chromosome and two ring plasmids. Two gene clusters, β-lactone and type III polyketide synthases (T3PKS), well-known for anti-toxic, anti-inflammatory, antioxidant, anticancer activities, were predicted through the antiSMASH database. In addition, probiotic genes for antioxidants, stress resistance, and anaerobic respiration were identified using the KEGG database. These results suggest that L. macroides G117 could be used as a probiotic in aquaculture. To further assess the probiotic properties of L. macroides G117, postbiotics were cultured and collected. Based on the survival rate of pesticide-stimulated A. salina, the optimum culture condition of L. macroides G117 was analysed. The optimal culture conditions were 1 % sucrose, 1.5 % yeast extract, 0.1 % MgSO4·7H2O, 30 °C, pH 7.8, bottled fluid volume 100 mL, shaking speed 180 rpm, inoculation quantity 0.5 %, and fermentation for 48 h, with a 15 % increase in survival rate of A. salina with the addition of optimised postbiotics. Untargeted metabolomic analysis revealed significant differences between the non-optimised and optimised postbiotics, mainly in amino acid and carbohydrate metabolites. Notably, postbiotics significantly increased the antioxidant enzymes in A. salina (catalase (CAT) and total superoxide dismutase (T-SOD)) and S. maximus (T-SOD) and decreased malondialdehyde (MDA) content. In addition, the activities of anaerobic respiratory-related enzymes (lactate dehydrogenase (LDH) and pyruvate kinase (PK)) were significantly increased, whereas those of aerobic respiratory-related enzymes (malate dehydrogenase (MDH)) were significantly decreased in S. maximus. These results suggest that L. macroides G117 postbiotics could improve the ... |
format |
Article in Journal/Newspaper |
author |
Shuo Qiao Chengjie Lv Xiaoli Zhang Xiaojing Lv Dinglong Yang Jianmin Zhao |
author_facet |
Shuo Qiao Chengjie Lv Xiaoli Zhang Xiaojing Lv Dinglong Yang Jianmin Zhao |
author_sort |
Shuo Qiao |
title |
Antioxidant and anti-stress properties of postbiotics produced by Lysinibacillus macroides G117 |
title_short |
Antioxidant and anti-stress properties of postbiotics produced by Lysinibacillus macroides G117 |
title_full |
Antioxidant and anti-stress properties of postbiotics produced by Lysinibacillus macroides G117 |
title_fullStr |
Antioxidant and anti-stress properties of postbiotics produced by Lysinibacillus macroides G117 |
title_full_unstemmed |
Antioxidant and anti-stress properties of postbiotics produced by Lysinibacillus macroides G117 |
title_sort |
antioxidant and anti-stress properties of postbiotics produced by lysinibacillus macroides g117 |
publisher |
Elsevier |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.cirep.2024.200143 https://doaj.org/article/b9f6491c928a4736977bc760ee4341ed |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_source |
Comparative Immunology Reports, Vol 6, Iss , Pp 200143- (2024) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2950311624000107 https://doaj.org/toc/2950-3116 2950-3116 doi:10.1016/j.cirep.2024.200143 https://doaj.org/article/b9f6491c928a4736977bc760ee4341ed |
op_doi |
https://doi.org/10.1016/j.cirep.2024.200143 |
container_title |
Comparative Immunology Reports |
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6 |
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200143 |
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1810475738489421824 |