Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis.
Metformin (Met) is an anti-hyperglycemic and potential anti-cancer agent which may exert its anti-proliferative effects via the induction of energetic stress. In this study we investigated the in vitro and in vivo efficacy of Met against the larval stage of Echinococcus granulosus. Metformin showed...
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ftdoajarticles:oai:doaj.org/article:b9ee9b3b442b4a5bb90fa9087c00c813 2023-05-15T15:07:18+02:00 Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis. Julia A Loos Valeria A Dávila Christian R Rodrígues Romina Petrigh Jorge A Zoppi Fernando A Crocenzi Andrea C Cumino 2017-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005370 https://doaj.org/article/b9ee9b3b442b4a5bb90fa9087c00c813 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5321462?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005370 https://doaj.org/article/b9ee9b3b442b4a5bb90fa9087c00c813 PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005370 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005370 2022-12-31T10:18:07Z Metformin (Met) is an anti-hyperglycemic and potential anti-cancer agent which may exert its anti-proliferative effects via the induction of energetic stress. In this study we investigated the in vitro and in vivo efficacy of Met against the larval stage of Echinococcus granulosus. Metformin showed significant dose- and time-dependent killing effects on in vitro cultured protoscoleces and metacestodes. Notably, the combination of Met together with the minimum effective concentration of ABZSO had a synergistic effect after days 3 and 12 on metacestodes and protoscoleces, respectively. Oral administration of Met (50 mg/kg/day) in E. granulosus-infected mice was highly effective in reducing the weight and number of parasite cysts, yet its combination with the lowest recommended dose of ABZ (5 mg/kg/day) was even more effective. Coincidentally, intracystic Met accumulation was higher in animals treated with both drugs compared to those administered Met alone. Furthermore, the safe plant-derived drug Met exhibited remarkable chemopreventive properties against secondary hydatidosis in mice. In conclusion, based on our experimental data, Met emerges as a promising anti-echinococcal drug as it has proven to efficiently inhibit the development and growth of the E. granulosus larval stage and its combination with ABZ may improve the current anti-parasitic therapy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 2 e0005370 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Julia A Loos Valeria A Dávila Christian R Rodrígues Romina Petrigh Jorge A Zoppi Fernando A Crocenzi Andrea C Cumino Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Metformin (Met) is an anti-hyperglycemic and potential anti-cancer agent which may exert its anti-proliferative effects via the induction of energetic stress. In this study we investigated the in vitro and in vivo efficacy of Met against the larval stage of Echinococcus granulosus. Metformin showed significant dose- and time-dependent killing effects on in vitro cultured protoscoleces and metacestodes. Notably, the combination of Met together with the minimum effective concentration of ABZSO had a synergistic effect after days 3 and 12 on metacestodes and protoscoleces, respectively. Oral administration of Met (50 mg/kg/day) in E. granulosus-infected mice was highly effective in reducing the weight and number of parasite cysts, yet its combination with the lowest recommended dose of ABZ (5 mg/kg/day) was even more effective. Coincidentally, intracystic Met accumulation was higher in animals treated with both drugs compared to those administered Met alone. Furthermore, the safe plant-derived drug Met exhibited remarkable chemopreventive properties against secondary hydatidosis in mice. In conclusion, based on our experimental data, Met emerges as a promising anti-echinococcal drug as it has proven to efficiently inhibit the development and growth of the E. granulosus larval stage and its combination with ABZ may improve the current anti-parasitic therapy. |
format |
Article in Journal/Newspaper |
author |
Julia A Loos Valeria A Dávila Christian R Rodrígues Romina Petrigh Jorge A Zoppi Fernando A Crocenzi Andrea C Cumino |
author_facet |
Julia A Loos Valeria A Dávila Christian R Rodrígues Romina Petrigh Jorge A Zoppi Fernando A Crocenzi Andrea C Cumino |
author_sort |
Julia A Loos |
title |
Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis. |
title_short |
Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis. |
title_full |
Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis. |
title_fullStr |
Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis. |
title_full_unstemmed |
Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis. |
title_sort |
metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005370 https://doaj.org/article/b9ee9b3b442b4a5bb90fa9087c00c813 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005370 (2017) |
op_relation |
http://europepmc.org/articles/PMC5321462?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005370 https://doaj.org/article/b9ee9b3b442b4a5bb90fa9087c00c813 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005370 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
2 |
container_start_page |
e0005370 |
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1766338841022562304 |