Cervical Dysplasia and Human Papillomavirus in Women of the Republic of Sakha (Yakutia)
The purpose of this study was to investigate the relation between the presence of cervical dysplasia and HPV infection in women of the Republic of Sakha (Yakutia) (RS(Y)), to analyze cytological samples for detecting the presence of pathology and the degree of cervical dysplasia, and to detect the l...
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International Medical Research and Development Corporation
2020
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Online Access: | https://doi.org/10.21103/Article10(2)_OA16 https://doaj.org/article/b9d77a16ce3d4b2999304050cdce2bae |
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ftdoajarticles:oai:doaj.org/article:b9d77a16ce3d4b2999304050cdce2bae 2023-05-15T18:06:42+02:00 Cervical Dysplasia and Human Papillomavirus in Women of the Republic of Sakha (Yakutia) Maria P. Kirillina Irina V. Kononova Sargylana I. Sofronova Matrena N. Mikhailova Varvara A. Vorontsova Elena L. Lushnikova 2020-06-01T00:00:00Z https://doi.org/10.21103/Article10(2)_OA16 https://doaj.org/article/b9d77a16ce3d4b2999304050cdce2bae EN eng International Medical Research and Development Corporation http://ijbm.org/articles/i38/ijbm_10(2)_oa16.pdf https://doaj.org/toc/2158-0510 https://doaj.org/toc/2158-0529 doi:10.21103/Article10(2)_OA16 2158-0510 2158-0529 https://doaj.org/article/b9d77a16ce3d4b2999304050cdce2bae International Journal of Biomedicine, Vol 10, Iss 2, Pp 165-168 (2020) human papillomavirus cervical cancer liquid-based cytology squamous intraepithelial lesion Medicine R article 2020 ftdoajarticles https://doi.org/10.21103/Article10(2)_OA16 2023-01-08T01:30:21Z The purpose of this study was to investigate the relation between the presence of cervical dysplasia and HPV infection in women of the Republic of Sakha (Yakutia) (RS(Y)), to analyze cytological samples for detecting the presence of pathology and the degree of cervical dysplasia, and to detect the level of HPV infection, and establish the dependence of HPV-test positivity on the presence of dysplasia and the woman’s age. Methods and Results: Cytological material was taken from cervix uteri and cervical canal scraping from 100 patients aged between 22 and 60 years (mean age of 38.9±9.2 years). The sample from every patient was obtained by traditional cytology and liquid-based cytology (LBC) on the automated system CellPrep Plus (Korea). The results of LBC were interpreted under the terminology system of the Bethesda System (2015). HPV detection and typing (types 6, 11, 44, 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) were performed by real-time PCR using commercial test systems according to their instructions. The analysis of cytological samples by the LBC method in women of RS(Y) revealed a high prevalence of NILM in the samples to compare with intraepithelial lesions of the cervix. Among intraepithelial lesions, LSIL was significantly more common. Positive HPV tests were observed in less than half of the cases. The vast majority of positive HPV tests were high–risk HPV types, and 16 and 51 types marked the most frequent. The positive tests for high–risk HPV types in women with NILM and intraepithelial lesions (LSIL, HSIL, ASCUS) were approximately equal. The frequency of positive tests for high–risk HPV types in women under 45 was higher than in women 46 years and older. Conclusion: The combined use of LBC and HPV testing for high–risk HPV infection improves the effectiveness of diagnostics by reducing the amount of uninformative material and allows detecting pathological changes at an earlier stage. Article in Journal/Newspaper Republic of Sakha Yakutia Directory of Open Access Journals: DOAJ Articles Sakha International Journal of Biomedicine 10 2 165 168 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
human papillomavirus cervical cancer liquid-based cytology squamous intraepithelial lesion Medicine R |
spellingShingle |
human papillomavirus cervical cancer liquid-based cytology squamous intraepithelial lesion Medicine R Maria P. Kirillina Irina V. Kononova Sargylana I. Sofronova Matrena N. Mikhailova Varvara A. Vorontsova Elena L. Lushnikova Cervical Dysplasia and Human Papillomavirus in Women of the Republic of Sakha (Yakutia) |
topic_facet |
human papillomavirus cervical cancer liquid-based cytology squamous intraepithelial lesion Medicine R |
description |
The purpose of this study was to investigate the relation between the presence of cervical dysplasia and HPV infection in women of the Republic of Sakha (Yakutia) (RS(Y)), to analyze cytological samples for detecting the presence of pathology and the degree of cervical dysplasia, and to detect the level of HPV infection, and establish the dependence of HPV-test positivity on the presence of dysplasia and the woman’s age. Methods and Results: Cytological material was taken from cervix uteri and cervical canal scraping from 100 patients aged between 22 and 60 years (mean age of 38.9±9.2 years). The sample from every patient was obtained by traditional cytology and liquid-based cytology (LBC) on the automated system CellPrep Plus (Korea). The results of LBC were interpreted under the terminology system of the Bethesda System (2015). HPV detection and typing (types 6, 11, 44, 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) were performed by real-time PCR using commercial test systems according to their instructions. The analysis of cytological samples by the LBC method in women of RS(Y) revealed a high prevalence of NILM in the samples to compare with intraepithelial lesions of the cervix. Among intraepithelial lesions, LSIL was significantly more common. Positive HPV tests were observed in less than half of the cases. The vast majority of positive HPV tests were high–risk HPV types, and 16 and 51 types marked the most frequent. The positive tests for high–risk HPV types in women with NILM and intraepithelial lesions (LSIL, HSIL, ASCUS) were approximately equal. The frequency of positive tests for high–risk HPV types in women under 45 was higher than in women 46 years and older. Conclusion: The combined use of LBC and HPV testing for high–risk HPV infection improves the effectiveness of diagnostics by reducing the amount of uninformative material and allows detecting pathological changes at an earlier stage. |
format |
Article in Journal/Newspaper |
author |
Maria P. Kirillina Irina V. Kononova Sargylana I. Sofronova Matrena N. Mikhailova Varvara A. Vorontsova Elena L. Lushnikova |
author_facet |
Maria P. Kirillina Irina V. Kononova Sargylana I. Sofronova Matrena N. Mikhailova Varvara A. Vorontsova Elena L. Lushnikova |
author_sort |
Maria P. Kirillina |
title |
Cervical Dysplasia and Human Papillomavirus in Women of the Republic of Sakha (Yakutia) |
title_short |
Cervical Dysplasia and Human Papillomavirus in Women of the Republic of Sakha (Yakutia) |
title_full |
Cervical Dysplasia and Human Papillomavirus in Women of the Republic of Sakha (Yakutia) |
title_fullStr |
Cervical Dysplasia and Human Papillomavirus in Women of the Republic of Sakha (Yakutia) |
title_full_unstemmed |
Cervical Dysplasia and Human Papillomavirus in Women of the Republic of Sakha (Yakutia) |
title_sort |
cervical dysplasia and human papillomavirus in women of the republic of sakha (yakutia) |
publisher |
International Medical Research and Development Corporation |
publishDate |
2020 |
url |
https://doi.org/10.21103/Article10(2)_OA16 https://doaj.org/article/b9d77a16ce3d4b2999304050cdce2bae |
geographic |
Sakha |
geographic_facet |
Sakha |
genre |
Republic of Sakha Yakutia |
genre_facet |
Republic of Sakha Yakutia |
op_source |
International Journal of Biomedicine, Vol 10, Iss 2, Pp 165-168 (2020) |
op_relation |
http://ijbm.org/articles/i38/ijbm_10(2)_oa16.pdf https://doaj.org/toc/2158-0510 https://doaj.org/toc/2158-0529 doi:10.21103/Article10(2)_OA16 2158-0510 2158-0529 https://doaj.org/article/b9d77a16ce3d4b2999304050cdce2bae |
op_doi |
https://doi.org/10.21103/Article10(2)_OA16 |
container_title |
International Journal of Biomedicine |
container_volume |
10 |
container_issue |
2 |
container_start_page |
165 |
op_container_end_page |
168 |
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1766178358441279488 |