Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers
Abstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approach...
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ftdoajarticles:oai:doaj.org/article:b9cca09659804219beb112709e29ac80 2023-05-15T13:26:50+02:00 Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers Silje Halvorsen Lars Korslund Morten Mattingsdal Audun Slettan 2023-02-01T00:00:00Z https://doi.org/10.1002/ece3.9785 https://doaj.org/article/b9cca09659804219beb112709e29ac80 EN eng Wiley https://doi.org/10.1002/ece3.9785 https://doaj.org/toc/2045-7758 2045-7758 doi:10.1002/ece3.9785 https://doaj.org/article/b9cca09659804219beb112709e29ac80 Ecology and Evolution, Vol 13, Iss 2, Pp n/a-n/a (2023) Anguilla anguilla conservation environmental DNA haplotype count population quantification Ecology QH540-549.5 article 2023 ftdoajarticles https://doi.org/10.1002/ece3.9785 2023-03-05T01:35:01Z Abstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel (Anguilla anguilla) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification. Article in Journal/Newspaper Anguilla anguilla European eel Directory of Open Access Journals: DOAJ Articles Ecology and Evolution 13 2 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anguilla anguilla conservation environmental DNA haplotype count population quantification Ecology QH540-549.5 |
spellingShingle |
Anguilla anguilla conservation environmental DNA haplotype count population quantification Ecology QH540-549.5 Silje Halvorsen Lars Korslund Morten Mattingsdal Audun Slettan Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
topic_facet |
Anguilla anguilla conservation environmental DNA haplotype count population quantification Ecology QH540-549.5 |
description |
Abstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel (Anguilla anguilla) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification. |
format |
Article in Journal/Newspaper |
author |
Silje Halvorsen Lars Korslund Morten Mattingsdal Audun Slettan |
author_facet |
Silje Halvorsen Lars Korslund Morten Mattingsdal Audun Slettan |
author_sort |
Silje Halvorsen |
title |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_short |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_full |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_fullStr |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_full_unstemmed |
Estimating number of European eel (Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_sort |
estimating number of european eel (anguilla anguilla) individuals using environmental dna and haplotype count in small rivers |
publisher |
Wiley |
publishDate |
2023 |
url |
https://doi.org/10.1002/ece3.9785 https://doaj.org/article/b9cca09659804219beb112709e29ac80 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Ecology and Evolution, Vol 13, Iss 2, Pp n/a-n/a (2023) |
op_relation |
https://doi.org/10.1002/ece3.9785 https://doaj.org/toc/2045-7758 2045-7758 doi:10.1002/ece3.9785 https://doaj.org/article/b9cca09659804219beb112709e29ac80 |
op_doi |
https://doi.org/10.1002/ece3.9785 |
container_title |
Ecology and Evolution |
container_volume |
13 |
container_issue |
2 |
_version_ |
1766394723745923072 |