1 H NMR-Based Metabolomics and Lipid Analyses Revealed the Effect of Dietary Replacement of Microbial Extracts or Mussel Meal with Fish Meal to Arctic Charr ( Salvelinus alpinus )
The effects of replacing 40% of dietary fish meal (FM) in a reference diet (REF) with either mussel meal (MM), zygomycete fungi (ZYG), extracted baker’s yeast (EY), or non-extracted baker’s yeast (NY) on the lipid and metabolic profile of Arctic charr ( Salvelinus alpinus ) were investigated. After...
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2019
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Online Access: | https://doi.org/10.3390/fishes4030046 https://doaj.org/article/b9c9b3131f2446a2a4a5e57373348523 |
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ftdoajarticles:oai:doaj.org/article:b9c9b3131f2446a2a4a5e57373348523 2023-05-15T14:30:01+02:00 1 H NMR-Based Metabolomics and Lipid Analyses Revealed the Effect of Dietary Replacement of Microbial Extracts or Mussel Meal with Fish Meal to Arctic Charr ( Salvelinus alpinus ) Liane Wagner Pedro Gómez-Requeni Ali A. Moazzami Torbjörn Lundh Aleksandar Vidakovic Markus Langeland Anders Kiessling Jana Pickova 2019-09-01T00:00:00Z https://doi.org/10.3390/fishes4030046 https://doaj.org/article/b9c9b3131f2446a2a4a5e57373348523 EN eng MDPI AG https://www.mdpi.com/2410-3888/4/3/46 https://doaj.org/toc/2410-3888 2410-3888 doi:10.3390/fishes4030046 https://doaj.org/article/b9c9b3131f2446a2a4a5e57373348523 Fishes, Vol 4, Iss 3, Pp 46-0 (2019) replacement baker’s yeast ( Saccharomyces cerevisiae ) zygomycete fungi ( Rhizopus oryzae ) fatty acids DHA fish Biology (General) QH301-705.5 Genetics QH426-470 article 2019 ftdoajarticles https://doi.org/10.3390/fishes4030046 2022-12-31T01:02:15Z The effects of replacing 40% of dietary fish meal (FM) in a reference diet (REF) with either mussel meal (MM), zygomycete fungi (ZYG), extracted baker’s yeast (EY), or non-extracted baker’s yeast (NY) on the lipid and metabolic profile of Arctic charr ( Salvelinus alpinus ) were investigated. After a 14-week feeding trial, liver and muscle tissues were collected for lipid (lipid content, lipid class, fatty acid composition) and 1 H NMR-based metabolomics analyses (aqueous and chloroform phases). Lipid analyses showed that fish fed ZYG diet had lower liver lipid content and thereby 10% higher level of docosahexaenoic acid compared with REF. Metabolomics analyses showed that on the one hand fish fed NY diet affected liver metabolites (2−3 fold higher concentrations of e.g., n,n-dimethylglycine and betaine) compared with REF, while, on the other hand, the muscle metabolic fingerprint was mainly affected by EY. In general, affected metabolites (e.g., alanine, anserine, betaine, hydroxyproline, isoleucine, malonate, n,n-dimethylglycine, proline, succinate, and valine) in fish fed test diets suggested that the test meal ingredients caused mainly a response in muscle metabolism. Fish metabolism was least affected by MM, which suggests that it may be suitable to replace fish meal in Arctic charr diets. Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Directory of Open Access Journals: DOAJ Articles Arctic Fishes 4 3 46 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
replacement baker’s yeast ( Saccharomyces cerevisiae ) zygomycete fungi ( Rhizopus oryzae ) fatty acids DHA fish Biology (General) QH301-705.5 Genetics QH426-470 |
spellingShingle |
replacement baker’s yeast ( Saccharomyces cerevisiae ) zygomycete fungi ( Rhizopus oryzae ) fatty acids DHA fish Biology (General) QH301-705.5 Genetics QH426-470 Liane Wagner Pedro Gómez-Requeni Ali A. Moazzami Torbjörn Lundh Aleksandar Vidakovic Markus Langeland Anders Kiessling Jana Pickova 1 H NMR-Based Metabolomics and Lipid Analyses Revealed the Effect of Dietary Replacement of Microbial Extracts or Mussel Meal with Fish Meal to Arctic Charr ( Salvelinus alpinus ) |
topic_facet |
replacement baker’s yeast ( Saccharomyces cerevisiae ) zygomycete fungi ( Rhizopus oryzae ) fatty acids DHA fish Biology (General) QH301-705.5 Genetics QH426-470 |
description |
The effects of replacing 40% of dietary fish meal (FM) in a reference diet (REF) with either mussel meal (MM), zygomycete fungi (ZYG), extracted baker’s yeast (EY), or non-extracted baker’s yeast (NY) on the lipid and metabolic profile of Arctic charr ( Salvelinus alpinus ) were investigated. After a 14-week feeding trial, liver and muscle tissues were collected for lipid (lipid content, lipid class, fatty acid composition) and 1 H NMR-based metabolomics analyses (aqueous and chloroform phases). Lipid analyses showed that fish fed ZYG diet had lower liver lipid content and thereby 10% higher level of docosahexaenoic acid compared with REF. Metabolomics analyses showed that on the one hand fish fed NY diet affected liver metabolites (2−3 fold higher concentrations of e.g., n,n-dimethylglycine and betaine) compared with REF, while, on the other hand, the muscle metabolic fingerprint was mainly affected by EY. In general, affected metabolites (e.g., alanine, anserine, betaine, hydroxyproline, isoleucine, malonate, n,n-dimethylglycine, proline, succinate, and valine) in fish fed test diets suggested that the test meal ingredients caused mainly a response in muscle metabolism. Fish metabolism was least affected by MM, which suggests that it may be suitable to replace fish meal in Arctic charr diets. |
format |
Article in Journal/Newspaper |
author |
Liane Wagner Pedro Gómez-Requeni Ali A. Moazzami Torbjörn Lundh Aleksandar Vidakovic Markus Langeland Anders Kiessling Jana Pickova |
author_facet |
Liane Wagner Pedro Gómez-Requeni Ali A. Moazzami Torbjörn Lundh Aleksandar Vidakovic Markus Langeland Anders Kiessling Jana Pickova |
author_sort |
Liane Wagner |
title |
1 H NMR-Based Metabolomics and Lipid Analyses Revealed the Effect of Dietary Replacement of Microbial Extracts or Mussel Meal with Fish Meal to Arctic Charr ( Salvelinus alpinus ) |
title_short |
1 H NMR-Based Metabolomics and Lipid Analyses Revealed the Effect of Dietary Replacement of Microbial Extracts or Mussel Meal with Fish Meal to Arctic Charr ( Salvelinus alpinus ) |
title_full |
1 H NMR-Based Metabolomics and Lipid Analyses Revealed the Effect of Dietary Replacement of Microbial Extracts or Mussel Meal with Fish Meal to Arctic Charr ( Salvelinus alpinus ) |
title_fullStr |
1 H NMR-Based Metabolomics and Lipid Analyses Revealed the Effect of Dietary Replacement of Microbial Extracts or Mussel Meal with Fish Meal to Arctic Charr ( Salvelinus alpinus ) |
title_full_unstemmed |
1 H NMR-Based Metabolomics and Lipid Analyses Revealed the Effect of Dietary Replacement of Microbial Extracts or Mussel Meal with Fish Meal to Arctic Charr ( Salvelinus alpinus ) |
title_sort |
1 h nmr-based metabolomics and lipid analyses revealed the effect of dietary replacement of microbial extracts or mussel meal with fish meal to arctic charr ( salvelinus alpinus ) |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://doi.org/10.3390/fishes4030046 https://doaj.org/article/b9c9b3131f2446a2a4a5e57373348523 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
Fishes, Vol 4, Iss 3, Pp 46-0 (2019) |
op_relation |
https://www.mdpi.com/2410-3888/4/3/46 https://doaj.org/toc/2410-3888 2410-3888 doi:10.3390/fishes4030046 https://doaj.org/article/b9c9b3131f2446a2a4a5e57373348523 |
op_doi |
https://doi.org/10.3390/fishes4030046 |
container_title |
Fishes |
container_volume |
4 |
container_issue |
3 |
container_start_page |
46 |
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1766303951088517120 |