Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania.
Background Brucellosis is a neglected zoonosis endemic in many countries, including regions of sub-Saharan Africa. Evaluated diagnostic tools for the detection of exposure to Brucella spp. are important for disease surveillance and guiding prevention and control activities. Methods and findings Baye...
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ftdoajarticles:oai:doaj.org/article:b9a7513ed12d438698ad4153b872908c 2023-05-15T15:14:55+02:00 Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania. Rebecca F Bodenham Stella Mazeri Sarah Cleaveland John A Crump Folorunso O Fasina William A de Glanville Daniel T Haydon Rudovick R Kazwala Tito J Kibona Venance P Maro Michael J Maze Blandina T Mmbaga Niwael J Mtui-Malamsha Gabriel M Shirima Emanuel S Swai Kate M Thomas Barend M deC Bronsvoort Jo E B Halliday 2021-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009630 https://doaj.org/article/b9a7513ed12d438698ad4153b872908c EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009630 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009630 https://doaj.org/article/b9a7513ed12d438698ad4153b872908c PLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009630 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009630 2022-12-31T16:13:26Z Background Brucellosis is a neglected zoonosis endemic in many countries, including regions of sub-Saharan Africa. Evaluated diagnostic tools for the detection of exposure to Brucella spp. are important for disease surveillance and guiding prevention and control activities. Methods and findings Bayesian latent class analysis was used to evaluate performance of the Rose Bengal plate test (RBT) and a competitive ELISA (cELISA) in detecting Brucella spp. exposure at the individual animal-level for cattle, sheep, and goats in Tanzania. Median posterior estimates of RBT sensitivity were: 0.779 (95% Bayesian credibility interval (BCI): 0.570-0.894), 0.893 (0.636-0.989), and 0.807 (0.575-0.966), and for cELISA were: 0.623 (0.443-0.790), 0.409 (0.241-0.644), and 0.561 (0.376-0.713), for cattle, sheep, and goats, respectively. Sensitivity BCIs were wide, with the widest for cELISA in sheep. RBT and cELISA median posterior estimates of specificity were high across species models: RBT ranged between 0.989 (0.980-0.998) and 0.995 (0.985-0.999), and cELISA between 0.984 (0.974-0.995) and 0.996 (0.988-1). Each species model generated seroprevalence estimates for two livestock subpopulations, pastoralist and non-pastoralist. Pastoralist seroprevalence estimates were: 0.063 (0.045-0.090), 0.033 (0.018-0.049), and 0.051 (0.034-0.076), for cattle, sheep, and goats, respectively. Non-pastoralist seroprevalence estimates were below 0.01 for all species models. Series and parallel diagnostic approaches were evaluated. Parallel outperformed a series approach. Median posterior estimates for parallel testing were ≥0.920 (0.760-0.986) for sensitivity and ≥0.973 (0.955-0.992) for specificity, for all species models. Conclusions Our findings indicate that Brucella spp. surveillance in Tanzania using RBT and cELISA in parallel at the animal-level would give high test performance. There is a need to evaluate strategies for implementing parallel testing at the herd- and flock-level. Our findings can assist in generating robust Brucella spp. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 8 e0009630 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rebecca F Bodenham Stella Mazeri Sarah Cleaveland John A Crump Folorunso O Fasina William A de Glanville Daniel T Haydon Rudovick R Kazwala Tito J Kibona Venance P Maro Michael J Maze Blandina T Mmbaga Niwael J Mtui-Malamsha Gabriel M Shirima Emanuel S Swai Kate M Thomas Barend M deC Bronsvoort Jo E B Halliday Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Brucellosis is a neglected zoonosis endemic in many countries, including regions of sub-Saharan Africa. Evaluated diagnostic tools for the detection of exposure to Brucella spp. are important for disease surveillance and guiding prevention and control activities. Methods and findings Bayesian latent class analysis was used to evaluate performance of the Rose Bengal plate test (RBT) and a competitive ELISA (cELISA) in detecting Brucella spp. exposure at the individual animal-level for cattle, sheep, and goats in Tanzania. Median posterior estimates of RBT sensitivity were: 0.779 (95% Bayesian credibility interval (BCI): 0.570-0.894), 0.893 (0.636-0.989), and 0.807 (0.575-0.966), and for cELISA were: 0.623 (0.443-0.790), 0.409 (0.241-0.644), and 0.561 (0.376-0.713), for cattle, sheep, and goats, respectively. Sensitivity BCIs were wide, with the widest for cELISA in sheep. RBT and cELISA median posterior estimates of specificity were high across species models: RBT ranged between 0.989 (0.980-0.998) and 0.995 (0.985-0.999), and cELISA between 0.984 (0.974-0.995) and 0.996 (0.988-1). Each species model generated seroprevalence estimates for two livestock subpopulations, pastoralist and non-pastoralist. Pastoralist seroprevalence estimates were: 0.063 (0.045-0.090), 0.033 (0.018-0.049), and 0.051 (0.034-0.076), for cattle, sheep, and goats, respectively. Non-pastoralist seroprevalence estimates were below 0.01 for all species models. Series and parallel diagnostic approaches were evaluated. Parallel outperformed a series approach. Median posterior estimates for parallel testing were ≥0.920 (0.760-0.986) for sensitivity and ≥0.973 (0.955-0.992) for specificity, for all species models. Conclusions Our findings indicate that Brucella spp. surveillance in Tanzania using RBT and cELISA in parallel at the animal-level would give high test performance. There is a need to evaluate strategies for implementing parallel testing at the herd- and flock-level. Our findings can assist in generating robust Brucella spp. ... |
format |
Article in Journal/Newspaper |
author |
Rebecca F Bodenham Stella Mazeri Sarah Cleaveland John A Crump Folorunso O Fasina William A de Glanville Daniel T Haydon Rudovick R Kazwala Tito J Kibona Venance P Maro Michael J Maze Blandina T Mmbaga Niwael J Mtui-Malamsha Gabriel M Shirima Emanuel S Swai Kate M Thomas Barend M deC Bronsvoort Jo E B Halliday |
author_facet |
Rebecca F Bodenham Stella Mazeri Sarah Cleaveland John A Crump Folorunso O Fasina William A de Glanville Daniel T Haydon Rudovick R Kazwala Tito J Kibona Venance P Maro Michael J Maze Blandina T Mmbaga Niwael J Mtui-Malamsha Gabriel M Shirima Emanuel S Swai Kate M Thomas Barend M deC Bronsvoort Jo E B Halliday |
author_sort |
Rebecca F Bodenham |
title |
Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania. |
title_short |
Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania. |
title_full |
Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania. |
title_fullStr |
Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania. |
title_full_unstemmed |
Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania. |
title_sort |
latent class evaluation of the performance of serological tests for exposure to brucella spp. in cattle, sheep, and goats in tanzania. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009630 https://doaj.org/article/b9a7513ed12d438698ad4153b872908c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009630 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009630 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009630 https://doaj.org/article/b9a7513ed12d438698ad4153b872908c |
op_doi |
https://doi.org/10.1371/journal.pntd.0009630 |
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PLOS Neglected Tropical Diseases |
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e0009630 |
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