Transcriptome analysis reveals high concentration of resveratrol promotes lipid synthesis and induces apoptosis in Siberian sturgeon (Acipenser baerii)

• Published in: BMC Genomics
• Main Authors: Shiyong Yang, Chaozhan Yan, Xiaojian Pang, Wuyuntana Shao, Zihan Xu, Datian Li, Wenqiang Xu, Zhehua Zhang, Boru Su, Yunkun Li, Jiayun Wu, Xiaoli Huang, Wei Luo, Xiaogang Du
• Format: Article in Journal/Newspaper
• Language: English
• Published: BMC 2024
• Subjects: Sturgeon; Resveratrol; Lipid metabolism; Apoptosis; Anti-oxidation; Biotechnology; TP248.13-248.65; Genetics; QH426-470; Acipenser baerii; Siberian sturgeon
• Online Access: https://doi.org/10.1186/s12864-024-10698-0; https://doaj.org/article/b99f7107b3694f65bd8b11e853729997

Abstract Resveratrol has been reported to promote immunity and decrease oxidative stress, but which demonstrates biphasic effects relied on the use concentration. In this study, the effects of diet supplement with a relative high concentration of resveratrol (0.32 mg/kg) on metabolism, antioxidation and apoptosis of liver were investigated in Siberian sturgeon. The results showed that resveratrol significantly increased the lipid synthesis and the apoptosis, but did not either activate the antioxidant NRF2/KEAP1 pathway or enhance the antioxidant enzyme activity. Transcriptome analysis revealed significant changes in regulatory pathways related to glycolipid, including PPAR signaling pathway, Insulin signaling pathway, Fatty acid biosynthesis, and Glycolysis/Gluconeogenesis. In addition, resveratrol significantly increased the lipid synthesis genes (accα and fas), fatty acid transport gene (fatp 6) and gluconeogenesis gene (gck), but decreased the survival-promoting genes (gadd45β and igf 1). These findings highlight a significant effect of resveratrol on glycolipid metabolism in Siberian sturgeon. Moreover, this study also demonstrated that 0.32 mg/kg resveratrol has physiological toxicity to the liver of Siberian sturgeon, indicating that this dose is too high for Siberian sturgeon. Thus, our study provides a valuable insight for future research and application of resveratrol in fish.